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Article: Manipulations of microRNA in human pluripotent stem cells and their derivatives
Title | Manipulations of microRNA in human pluripotent stem cells and their derivatives |
---|---|
Authors | |
Keywords | Directed Differentiation Human Embryonic Stem Cells Microrna Rna Interference |
Issue Date | 2011 |
Citation | Methods In Molecular Biology, 2011, v. 690, p. 107-120 How to Cite? |
Abstract | Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells can self-renew while maintaining their pluripotency to differentiate into virtually all cell types. In addition to their potential for regenerative medicine, hESCs and iPSCs can also serve as excellent in vitro models for the study of human organogenesis and disease models, as well as drug toxicity screening. MicroRNAs (miRNAs) are nonencoding RNAs of ∼22 nucleotides that function as negative transcriptional regulators via degradation or inhibition by RNA interference (RNAi). MiRNAs play essential roles in developmental pathways. This chapter provides a description of how miRNAs can be introduced into hESCs/iPSCs or their derivatives for experiments via lentivirus-mediated gene transfer. © 2011 Springer Science+Business Media, LLC. |
Persistent Identifier | http://hdl.handle.net/10722/163367 |
ISSN | 2023 SCImago Journal Rankings: 0.399 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rushing, SN | en_US |
dc.contributor.author | Herren, AW | en_US |
dc.contributor.author | Lieu, DK | en_US |
dc.contributor.author | Li, RA | en_US |
dc.date.accessioned | 2012-09-05T05:30:37Z | - |
dc.date.available | 2012-09-05T05:30:37Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | Methods In Molecular Biology, 2011, v. 690, p. 107-120 | en_US |
dc.identifier.issn | 1064-3745 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/163367 | - |
dc.description.abstract | Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells can self-renew while maintaining their pluripotency to differentiate into virtually all cell types. In addition to their potential for regenerative medicine, hESCs and iPSCs can also serve as excellent in vitro models for the study of human organogenesis and disease models, as well as drug toxicity screening. MicroRNAs (miRNAs) are nonencoding RNAs of ∼22 nucleotides that function as negative transcriptional regulators via degradation or inhibition by RNA interference (RNAi). MiRNAs play essential roles in developmental pathways. This chapter provides a description of how miRNAs can be introduced into hESCs/iPSCs or their derivatives for experiments via lentivirus-mediated gene transfer. © 2011 Springer Science+Business Media, LLC. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Methods in Molecular Biology | en_US |
dc.subject | Directed Differentiation | en_US |
dc.subject | Human Embryonic Stem Cells | en_US |
dc.subject | Microrna | en_US |
dc.subject | Rna Interference | en_US |
dc.title | Manipulations of microRNA in human pluripotent stem cells and their derivatives | en_US |
dc.type | Article | en_US |
dc.identifier.email | Li, RA:ronaldli@hkucc.hku.hk | en_US |
dc.identifier.authority | Li, RA=rp01352 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/978-1-60761-962-8_7 | en_US |
dc.identifier.scopus | eid_2-s2.0-79952202114 | en_US |
dc.identifier.hkuros | 239700 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79952202114&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 690 | en_US |
dc.identifier.spage | 107 | en_US |
dc.identifier.epage | 120 | en_US |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Rushing, SN=25121769500 | en_US |
dc.identifier.scopusauthorid | Herren, AW=37004474100 | en_US |
dc.identifier.scopusauthorid | Lieu, DK=7003924538 | en_US |
dc.identifier.scopusauthorid | Li, RA=7404724466 | en_US |
dc.identifier.issnl | 1064-3745 | - |