File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1007/s00384-008-0566-1
- Scopus: eid_2-s2.0-59649112825
- PMID: 18704457
- WOS: WOS:000262986000001
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Gene expression profile in colon cancer cells with respect to XIAP expression status
Title | Gene expression profile in colon cancer cells with respect to XIAP expression status |
---|---|
Authors | |
Keywords | Angiogenesis Apoptosis Colon cancer Geneexpression Microarray PPARγ Proliferation XIAP |
Issue Date | 2009 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00384/index.htm |
Citation | International Journal Of Colorectal Disease, 2009, v. 24 n. 3, p. 245-260 How to Cite? |
Abstract | Background and aims: We observed a marked synergism between peroxisome proliferator-activated receptor gamma (PPARγ) ligands and X-linked inhibitor of apoptosis (XIAP) down-regulation in colon cancer. In the current study, we detected the gene expression profile in HCT116 cells treated with or without PPARγ ligand troglitazone. Materials and methods: HCT116-XIAP +/+ and HCT116-XIAP -/- cells were treated with or without 50 μM troglitazone for 48 h. Gene expressions were detected by microarray, and selected genes were validated by reverse-transcriptase polymerase chain reaction (PCR), real-time PCR, and Western blot. Results: Relative to HCT116-XIAP +/+ cells, 58 genes were up-regulated and 33 genes down-regulated in HCT116-XIAP -/- cells, all by ≥4-fold. These genes could be classified into a wide variety of functional classes, but we focused on those related to angiogenesis, apoptosis, and proliferation. Thus, two pro-apoptotic genes and one pro-proliferation gene were up-regulated in HCT116-XIAP -/- cells. Two pro-proliferation genes, one pro-angiogenesis gene, one anti-angiogenesis gene, and one anti-apoptosis gene were down-regulated in HCT116-XIAP -/- cells. Relative to HCT116-XIAP +/+ cells treated with troglitazone, 137 genes were up-regulated, and 31 genes were down-regulated in troglitazone-treated HCT116-XIAP -/- cells, all by ≥4-fold. Among the up-regulated genes were two anti-angiogenesis genes, seven pro-apoptosis genes, and six anti-proliferation genes. Among the down-regulated genes were one anti-angiogenesis gene, one pro-angiogenesis gene, one anti-apoptosis gene, one anti-proliferation gene, and two pro-proliferation genes. Conclusion: Down-regulation of XIAP in HCT116 cells with or without troglitazone treatment was associated with changes of gene expression that favor increased tendency of apoptosis, decreased cell proliferation, and angiogenesis potential. © Springer-Verlag 2008. |
Persistent Identifier | http://hdl.handle.net/10722/163225 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.911 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Qiao, L | en_HK |
dc.contributor.author | Li, GHY | en_HK |
dc.contributor.author | Dai, Y | en_HK |
dc.contributor.author | Wang, J | en_HK |
dc.contributor.author | Li, Z | en_HK |
dc.contributor.author | Zou, B | en_HK |
dc.contributor.author | Gu, Q | en_HK |
dc.contributor.author | Ma, J | en_HK |
dc.contributor.author | Pang, R | en_HK |
dc.contributor.author | Lan, HY | en_HK |
dc.contributor.author | Wong, BCY | en_HK |
dc.date.accessioned | 2012-09-05T05:28:55Z | - |
dc.date.available | 2012-09-05T05:28:55Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | International Journal Of Colorectal Disease, 2009, v. 24 n. 3, p. 245-260 | en_HK |
dc.identifier.issn | 0179-1958 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/163225 | - |
dc.description.abstract | Background and aims: We observed a marked synergism between peroxisome proliferator-activated receptor gamma (PPARγ) ligands and X-linked inhibitor of apoptosis (XIAP) down-regulation in colon cancer. In the current study, we detected the gene expression profile in HCT116 cells treated with or without PPARγ ligand troglitazone. Materials and methods: HCT116-XIAP +/+ and HCT116-XIAP -/- cells were treated with or without 50 μM troglitazone for 48 h. Gene expressions were detected by microarray, and selected genes were validated by reverse-transcriptase polymerase chain reaction (PCR), real-time PCR, and Western blot. Results: Relative to HCT116-XIAP +/+ cells, 58 genes were up-regulated and 33 genes down-regulated in HCT116-XIAP -/- cells, all by ≥4-fold. These genes could be classified into a wide variety of functional classes, but we focused on those related to angiogenesis, apoptosis, and proliferation. Thus, two pro-apoptotic genes and one pro-proliferation gene were up-regulated in HCT116-XIAP -/- cells. Two pro-proliferation genes, one pro-angiogenesis gene, one anti-angiogenesis gene, and one anti-apoptosis gene were down-regulated in HCT116-XIAP -/- cells. Relative to HCT116-XIAP +/+ cells treated with troglitazone, 137 genes were up-regulated, and 31 genes were down-regulated in troglitazone-treated HCT116-XIAP -/- cells, all by ≥4-fold. Among the up-regulated genes were two anti-angiogenesis genes, seven pro-apoptosis genes, and six anti-proliferation genes. Among the down-regulated genes were one anti-angiogenesis gene, one pro-angiogenesis gene, one anti-apoptosis gene, one anti-proliferation gene, and two pro-proliferation genes. Conclusion: Down-regulation of XIAP in HCT116 cells with or without troglitazone treatment was associated with changes of gene expression that favor increased tendency of apoptosis, decreased cell proliferation, and angiogenesis potential. © Springer-Verlag 2008. | en_HK |
dc.language | eng | en_US |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00384/index.htm | en_HK |
dc.relation.ispartof | International Journal of Colorectal Disease | en_HK |
dc.subject | Angiogenesis | en_HK |
dc.subject | Apoptosis | en_HK |
dc.subject | Colon cancer | en_HK |
dc.subject | Geneexpression | en_HK |
dc.subject | Microarray | en_HK |
dc.subject | PPARγ | en_HK |
dc.subject | Proliferation | en_HK |
dc.subject | XIAP | en_HK |
dc.subject.mesh | Apoptosis - Drug Effects - Genetics | en_US |
dc.subject.mesh | Blotting, Western | en_US |
dc.subject.mesh | Cell Proliferation - Drug Effects | en_US |
dc.subject.mesh | Chromans - Pharmacology | en_US |
dc.subject.mesh | Colonic Neoplasms - Genetics - Pathology | en_US |
dc.subject.mesh | Gene Expression Profiling | en_US |
dc.subject.mesh | Gene Expression Regulation, Neoplastic - Drug Effects | en_US |
dc.subject.mesh | Hct116 Cells | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Neovascularization, Pathologic - Genetics | en_US |
dc.subject.mesh | Oligonucleotide Array Sequence Analysis | en_US |
dc.subject.mesh | Reproducibility Of Results | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Thiazolidinediones - Pharmacology | en_US |
dc.subject.mesh | X-Linked Inhibitor Of Apoptosis Protein - Genetics - Metabolism | en_US |
dc.title | Gene expression profile in colon cancer cells with respect to XIAP expression status | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Qiao, L: lq8688@hotmail.com | en_HK |
dc.identifier.email | Wang, J: jidewang@gmail.com | en_HK |
dc.identifier.email | Pang, R: robertap@hku.hk | en_HK |
dc.identifier.email | Wong, BCY: bcywong@hku.hk | en_HK |
dc.identifier.authority | Qiao, L=rp00513 | en_HK |
dc.identifier.authority | Wang, J=rp00491 | en_HK |
dc.identifier.authority | Pang, R=rp00274 | en_HK |
dc.identifier.authority | Wong, BCY=rp00429 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/s00384-008-0566-1 | en_HK |
dc.identifier.pmid | 18704457 | - |
dc.identifier.scopus | eid_2-s2.0-59649112825 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-59649112825&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 24 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 245 | en_HK |
dc.identifier.epage | 260 | en_HK |
dc.identifier.isi | WOS:000262986000001 | - |
dc.publisher.place | Germany | en_HK |
dc.identifier.scopusauthorid | Qiao, L=7202151719 | en_HK |
dc.identifier.scopusauthorid | Li, GHY=35080710200 | en_HK |
dc.identifier.scopusauthorid | Dai, Y=7401512993 | en_HK |
dc.identifier.scopusauthorid | Wang, J=35309087500 | en_HK |
dc.identifier.scopusauthorid | Li, Z=24171072000 | en_HK |
dc.identifier.scopusauthorid | Zou, B=35228257300 | en_HK |
dc.identifier.scopusauthorid | Gu, Q=24469982400 | en_HK |
dc.identifier.scopusauthorid | Ma, J=35275386200 | en_HK |
dc.identifier.scopusauthorid | Pang, R=7004376659 | en_HK |
dc.identifier.scopusauthorid | Lan, HY=7102710832 | en_HK |
dc.identifier.scopusauthorid | Wong, BCY=7402023340 | en_HK |
dc.identifier.citeulike | 4162296 | - |
dc.identifier.issnl | 0179-1958 | - |