File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Maternal nutritional status and its effect on hepatic and adipose tissue FAS activity in the offspring

TitleMaternal nutritional status and its effect on hepatic and adipose tissue FAS activity in the offspring
Authors
Issue Date1996
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
Faseb Journal, 1996, v. 10 n. 3, p. A519 How to Cite?
AbstractFatty acid synthetase (FAS) expression and activity is controlled by nutritional and hormonal status of the animal. Whether there is a long term nutritional effect on the activity and expression of this enzyme is not well studied. To study the short and long term effects of different plane of maternal nutrition during gestation and lactation on hepatic and adipose tissue FAS activity of the offsprings, female rats were randomly assigned on day of mating to one of four different dietary groups: Gpl (control, fed ad libitum), Gp2 (fed 70% of control intake during gestation and lactation), Gp3 (fed 70% during gestation and 100 % during lactation) Litter size was adjusted to 10 pups on day of birth. Pups were weaned to an ad libitum commercial rat chow on day 20. On days 20 and 90, hepatic and adipose tissue lipogenic activity were measured by fatty acid incorporation of tritiated water, while FAS activity was measured through the malonyl and acetyl CoA- dependent rate of NADPH oxidation by spectrophotometric method. Hepatic lipogenic activity, expressed as total activity, was significantly lower at 20 days in Gp2 (73.84±11) compared with Gpl(114.08±16), (Student t-test, p<0.05). No differences were noted among other groups at this date and for all groups at 90 days. Results of FAS assay expressed as nmol NADPH/min/mg protein are as follows: Group Hepatic tissue Adipose tissue Day 20 Day 90 Day 20 Day 90 1 3.31±0.25 2.30±0.20 3.15±0.25 2.41±0.11 2 3.98±0.30 2.15±0.27 3.12±1.28 2.27±.0.40 3 3.57±0.15 2.22±0.09 3.38±0.21 2.71±0.29 Our result showed that although the activity of FAS was lower at 90 days, there was no difference among groups at both time point. This suggest that maternal nutritional status has no long term effect on hepatic and adipose tissue lipogenic activity of the offspring.
Persistent Identifierhttp://hdl.handle.net/10722/163027
ISSN
2023 Impact Factor: 4.4
2023 SCImago Journal Rankings: 1.412

 

DC FieldValueLanguage
dc.contributor.authorBalonan, LCen_US
dc.contributor.authorCheung, TFen_US
dc.contributor.authorSheng, HPen_US
dc.date.accessioned2012-09-05T05:26:44Z-
dc.date.available2012-09-05T05:26:44Z-
dc.date.issued1996en_US
dc.identifier.citationFaseb Journal, 1996, v. 10 n. 3, p. A519en_US
dc.identifier.issn0892-6638en_US
dc.identifier.urihttp://hdl.handle.net/10722/163027-
dc.description.abstractFatty acid synthetase (FAS) expression and activity is controlled by nutritional and hormonal status of the animal. Whether there is a long term nutritional effect on the activity and expression of this enzyme is not well studied. To study the short and long term effects of different plane of maternal nutrition during gestation and lactation on hepatic and adipose tissue FAS activity of the offsprings, female rats were randomly assigned on day of mating to one of four different dietary groups: Gpl (control, fed ad libitum), Gp2 (fed 70% of control intake during gestation and lactation), Gp3 (fed 70% during gestation and 100 % during lactation) Litter size was adjusted to 10 pups on day of birth. Pups were weaned to an ad libitum commercial rat chow on day 20. On days 20 and 90, hepatic and adipose tissue lipogenic activity were measured by fatty acid incorporation of tritiated water, while FAS activity was measured through the malonyl and acetyl CoA- dependent rate of NADPH oxidation by spectrophotometric method. Hepatic lipogenic activity, expressed as total activity, was significantly lower at 20 days in Gp2 (73.84±11) compared with Gpl(114.08±16), (Student t-test, p<0.05). No differences were noted among other groups at this date and for all groups at 90 days. Results of FAS assay expressed as nmol NADPH/min/mg protein are as follows: Group Hepatic tissue Adipose tissue Day 20 Day 90 Day 20 Day 90 1 3.31±0.25 2.30±0.20 3.15±0.25 2.41±0.11 2 3.98±0.30 2.15±0.27 3.12±1.28 2.27±.0.40 3 3.57±0.15 2.22±0.09 3.38±0.21 2.71±0.29 Our result showed that although the activity of FAS was lower at 90 days, there was no difference among groups at both time point. This suggest that maternal nutritional status has no long term effect on hepatic and adipose tissue lipogenic activity of the offspring.en_US
dc.languageengen_US
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_US
dc.relation.ispartofFASEB Journalen_US
dc.titleMaternal nutritional status and its effect on hepatic and adipose tissue FAS activity in the offspringen_US
dc.typeArticleen_US
dc.identifier.emailCheung, TF:rtcheung@hku.hken_US
dc.identifier.authorityCheung, TF=rp00434en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-33749131979en_US
dc.identifier.volume10en_US
dc.identifier.issue3en_US
dc.identifier.spageA519en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBalonan, LC=6504667340en_US
dc.identifier.scopusauthoridCheung, TF=7202397498en_US
dc.identifier.scopusauthoridSheng, HP=7201933542en_US
dc.identifier.issnl0892-6638-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats