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Article: Quantitative detection of methylated SOCS-1 , a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection.

TitleQuantitative detection of methylated SOCS-1 , a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection.
Authors
Chan, MWChu, ESTo, KFLeung, WK
Issue Date2004
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492
Citation
Biotechnology Letters, 2004, v. 26 n. 16, p. 1289-1293 How to Cite?
DOI: http://dx.doi.org/10.1023/B:BILE.0000044922.43572.2d
AbstractAlthough methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.
Persistent Identifierhttp://hdl.handle.net/10722/162819
ISSN
0141-5492
2023 Impact Factor: 2.0
2023 SCImago Journal Rankings: 0.519
ISI Accession Number ID
WOS:000224962100008

 

DC FieldValueLanguage
dc.contributor.authorChan, MWen_US
dc.contributor.authorChu, ESen_US
dc.contributor.authorTo, KFen_US
dc.contributor.authorLeung, WKen_US
dc.date.accessioned2012-09-05T05:23:56Z-
dc.date.available2012-09-05T05:23:56Z-
dc.date.issued2004en_US
dc.identifier.citationBiotechnology Letters, 2004, v. 26 n. 16, p. 1289-1293en_US
dc.identifier.issn0141-5492en_US
dc.identifier.urihttp://hdl.handle.net/10722/162819-
dc.description.abstractAlthough methylation-specific PCR (MSP) is a sensitive technique in the detection of DNA hypermethylation, it is not quantitative. Here we described a modified PCR protocol to quantify methylated SOCS-1 gene by real time MSP using SYBR green, which involves an additional PCR step after the 72 degrees C extension step. This modified protocol is also useful in the quantitative detection of methylated SOCS-1 gene in serum samples of gastric cancer patients.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492en_US
dc.relation.ispartofBiotechnology lettersen_US
dc.subject.meshAdulten_US
dc.subject.meshDna Methylationen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenes, Tumor Suppressoren_US
dc.subject.meshGenetic Predisposition To Disease - Geneticsen_US
dc.subject.meshGenetic Testing - Methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshIntracellular Signaling Peptides And Proteins - Blood - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMethylationen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshOrganic Chemicalsen_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshRepressor Proteins - Blood - Metabolismen_US
dc.subject.meshReproducibility Of Resultsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshStomach Neoplasms - Blood - Genetics - Metabolismen_US
dc.subject.meshSuppressor Of Cytokine Signaling Proteinsen_US
dc.subject.meshTumor Markers, Biological - Blooden_US
dc.titleQuantitative detection of methylated SOCS-1 , a tumor suppressor gene, by a modified protocol of quantitative real time methylation-specific PCR using SYBR green and its use in early gastric cancer detection.en_US
dc.typeArticleen_US
dc.identifier.emailLeung, WK:waikleung@hku.hken_US
dc.identifier.authorityLeung, WK=rp01479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1023/B:BILE.0000044922.43572.2d-
dc.identifier.pmid15483389-
dc.identifier.scopuseid_2-s2.0-16544367808en_US
dc.identifier.volume26en_US
dc.identifier.issue16en_US
dc.identifier.spage1289en_US
dc.identifier.epage1293en_US
dc.identifier.isiWOS:000224962100008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridChan, MW=7402597788en_US
dc.identifier.scopusauthoridChu, ES=8631130300en_US
dc.identifier.scopusauthoridTo, KF=7101911940en_US
dc.identifier.scopusauthoridLeung, WK=7201504523en_US
dc.identifier.issnl0141-5492-

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