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Conference Paper: Antioxidants N-acetylcysterine and Allopurinol synergistically enhance cardiac HIF-1α and heme oxygenase-1 and attenuate Postischemic Myocardial Injury in diabetic rats
Title | Antioxidants N-acetylcysterine and Allopurinol synergistically enhance cardiac HIF-1α and heme oxygenase-1 and attenuate Postischemic Myocardial Injury in diabetic rats |
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Authors | |
Issue Date | 2012 |
Publisher | Federation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/ |
Citation | The 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 n. S1, abstract no. 1114.3 How to Cite? |
Abstract | Hypoxia inducible factor 1(HIF) α can reduce myocardial ischemia-reperfusion injury(MIRI). However, hyperglycemia-induced oxidative stress may reduce cardiac HIF-1α and subsequently inhibit heme oxygenase 1 (HO-1), a down-stream protein of HIF-1α that has anti-ischemic properties. N-acetylcysteine (NAC) and allopurinol (ALP) synergistically reduce MIRI in diabetes (PLoS One. 2011;6:e23967), but the role of HIF-1α/HO-1 in this process in unknown. Control or streptozotocin (STZ)-induced diabetic rats were untreated (C, D) or treated with NAC (1.5g/kg/day) or ALP (100 mg/kg/day) or their combination (NAC+ALP) for four weeks starting one week after STZ injection. Cardiac and plasma 15-F2t-isoprostane (IsoP) were increased in D rats while cardiac HO-1 and protein expression and activity were reduced, accompanied with reduced cardiac HIF-1α, and increased post-ischemic myocardial infarct size (IS) and cellular injury in D rats subjected to 30 minutes of coronary artery occlusion and 2 hours of reperfusion (all P<0.05 D vs. C). NAC+ALP normalized cardiac levels of HO-1 and HIF-1α protein expression, prevented the increase in IsoP, and reduced myocardial IS, but these effects of NAC+ALP were cancelled by either the HO-1 blocker protoporphyrin or the HIF-1α blocker 2-Methoxyestradiol. It is concluded that HIF-1α and HO-1 activation play an important role in NAC and ALP mediated cardioprotection in diabetes. |
Persistent Identifier | http://hdl.handle.net/10722/160421 |
ISSN | 2023 Impact Factor: 4.4 2023 SCImago Journal Rankings: 1.412 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Mao, X | en_US |
dc.contributor.author | Liu, Y | en_US |
dc.contributor.author | Wang, T | en_US |
dc.contributor.author | Lei, S | en_US |
dc.contributor.author | Irwin, MG | en_US |
dc.contributor.author | Vanhoutte, PM | en_US |
dc.contributor.author | Xia, Z | en_US |
dc.date.accessioned | 2012-08-16T06:10:47Z | - |
dc.date.available | 2012-08-16T06:10:47Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | The 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 n. S1, abstract no. 1114.3 | en_US |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10722/160421 | - |
dc.description.abstract | Hypoxia inducible factor 1(HIF) α can reduce myocardial ischemia-reperfusion injury(MIRI). However, hyperglycemia-induced oxidative stress may reduce cardiac HIF-1α and subsequently inhibit heme oxygenase 1 (HO-1), a down-stream protein of HIF-1α that has anti-ischemic properties. N-acetylcysteine (NAC) and allopurinol (ALP) synergistically reduce MIRI in diabetes (PLoS One. 2011;6:e23967), but the role of HIF-1α/HO-1 in this process in unknown. Control or streptozotocin (STZ)-induced diabetic rats were untreated (C, D) or treated with NAC (1.5g/kg/day) or ALP (100 mg/kg/day) or their combination (NAC+ALP) for four weeks starting one week after STZ injection. Cardiac and plasma 15-F2t-isoprostane (IsoP) were increased in D rats while cardiac HO-1 and protein expression and activity were reduced, accompanied with reduced cardiac HIF-1α, and increased post-ischemic myocardial infarct size (IS) and cellular injury in D rats subjected to 30 minutes of coronary artery occlusion and 2 hours of reperfusion (all P<0.05 D vs. C). NAC+ALP normalized cardiac levels of HO-1 and HIF-1α protein expression, prevented the increase in IsoP, and reduced myocardial IS, but these effects of NAC+ALP were cancelled by either the HO-1 blocker protoporphyrin or the HIF-1α blocker 2-Methoxyestradiol. It is concluded that HIF-1α and HO-1 activation play an important role in NAC and ALP mediated cardioprotection in diabetes. | - |
dc.language | eng | en_US |
dc.publisher | Federation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/ | - |
dc.relation.ispartof | The FASEB Journal | en_US |
dc.title | Antioxidants N-acetylcysterine and Allopurinol synergistically enhance cardiac HIF-1α and heme oxygenase-1 and attenuate Postischemic Myocardial Injury in diabetic rats | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Liu, Y: yanan@hku.hk | en_US |
dc.identifier.email | Wang, T: wangtt6@hku.hk | en_US |
dc.identifier.email | Lei, S: shqlei@hku.hk | en_US |
dc.identifier.email | Irwin, MG: mgirwin@hku.hk | en_US |
dc.identifier.email | Vanhoutte, PM: vanhoutt@hku.hk | en_US |
dc.identifier.email | Xia, Z: zyxia@hkucc.hku.hk | - |
dc.identifier.authority | Irwin, MG=rp00390 | en_US |
dc.identifier.authority | Vanhoutte, PM=rp00238 | en_US |
dc.identifier.authority | Xia, Z=rp00532 | en_US |
dc.description.nature | abstract | - |
dc.identifier.doi | 10.1096/fasebj.26.1_supplement.1114.3 | - |
dc.identifier.hkuros | 205200 | en_US |
dc.identifier.volume | 26 | en_US |
dc.identifier.issue | S1 | - |
dc.identifier.spage | abstract no. 1114.3 | - |
dc.identifier.epage | abstract no. 1114.3 | - |
dc.identifier.isi | WOS:000310711304152 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0892-6638 | - |