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Conference Paper: Migratory response of human orbital fat-derived mesenchymal stem cell encapsulated in collagen gel

TitleMigratory response of human orbital fat-derived mesenchymal stem cell encapsulated in collagen gel
Authors
Diao, HJYu, CSChan, BP
Keywords3D-migrated
migration
OFSC
selection barrier
Issue Date2010
Citation
The 4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Conference Proceedings, 2010, p. 231-234 How to Cite?
DOI: http://dx.doi.org/10.1109/NANOMED.2010.5749841
AbstractThe aim of this study is to make a functional selection of the highly migratory orbital fat-derived stem cells (OFSCs) using a 3D collagen gel as a selection barrier. Firstly we isolated OFSCs by regular digestion. Passage 2-3 OFSCs were used to verify their multiple differentiation potentials. Then we encapsulated passage 2-6 OFSCs into collagen type I microspheres, followed by a well-established cell migration assay under attraction of two kinds of chemokines - 10 ng/ml Fractalkine or 50 ng/ml SDF-1β. After staining and counting, we found that after 3D collagen barrier selection, migrated OFSCs have more sensitive response to the induction of these two chemokines. © 2010 IEEE.
Persistent Identifierhttp://hdl.handle.net/10722/159031
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorDiao, HJen_HK
dc.contributor.authorYu, CSen_HK
dc.contributor.authorChan, BPen_HK
dc.date.accessioned2012-08-08T09:05:14Z-
dc.date.available2012-08-08T09:05:14Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010), Hong Kong/Macau, China, 5-9 December 2010. In Conference Proceedings, 2010, p. 231-234en_US
dc.identifier.urihttp://hdl.handle.net/10722/159031-
dc.description.abstractThe aim of this study is to make a functional selection of the highly migratory orbital fat-derived stem cells (OFSCs) using a 3D collagen gel as a selection barrier. Firstly we isolated OFSCs by regular digestion. Passage 2-3 OFSCs were used to verify their multiple differentiation potentials. Then we encapsulated passage 2-6 OFSCs into collagen type I microspheres, followed by a well-established cell migration assay under attraction of two kinds of chemokines - 10 ng/ml Fractalkine or 50 ng/ml SDF-1β. After staining and counting, we found that after 3D collagen barrier selection, migrated OFSCs have more sensitive response to the induction of these two chemokines. © 2010 IEEE.en_HK
dc.languageengen_US
dc.relation.ispartofIEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010en_HK
dc.subject3D-migrateden_HK
dc.subjectmigrationen_HK
dc.subjectOFSCen_HK
dc.subjectselection barrieren_HK
dc.titleMigratory response of human orbital fat-derived mesenchymal stem cell encapsulated in collagen gelen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYu, CS: carolsyu@hku.hken_HK
dc.identifier.emailChan, BP: bpchan@hkucc.hku.hken_HK
dc.identifier.authorityYu, CS=rp00305en_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1109/NANOMED.2010.5749841en_HK
dc.identifier.scopuseid_2-s2.0-79956049060en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79956049060&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.spage231en_HK
dc.identifier.epage234en_HK
dc.identifier.scopusauthoridDiao, HJ=38861394000en_HK
dc.identifier.scopusauthoridYu, CS=8856262400en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK
dc.customcontrol.immutablesml 160518 amended-

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