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- Scopus: eid_2-s2.0-33749048265
- PMID: 16957379
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Article: MicroRNAs in human immunodeficiency virus-1 infection.
Title | MicroRNAs in human immunodeficiency virus-1 infection. |
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Authors | |
Issue Date | 2006 |
Citation | Methods In Molecular Biology (Clifton, N.J.), 2006, v. 342, p. 241-253 How to Cite? |
Abstract | Initially reported for Caenorhabditis elegans, microRNA (miRNA) has been shown to regulate gene expression in plants, flies, and mammals . Here, we report on our approaches to investigate the role of miRNAs in human immunodeficiency virus (HIV)-1 infection. Using computer-directed foldings, we first identify potential sequences in HIV-1 that putatively encode miRNAs. Subsequently, we use Northern blotting of RNAs isolated from HIV-infected cells to confirm expression of predicted miRNA sequences. Finally, we use a scanning algorithm to search 3' untranslated regions (UTRs) of human messenger RNAs (mRNAs) in the attempt to predict potential sites targeted by HIV-1 miRNAs. |
Persistent Identifier | http://hdl.handle.net/10722/157461 |
ISSN | 2023 SCImago Journal Rankings: 0.399 |
DC Field | Value | Language |
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dc.contributor.author | Bennasser, Y | en_US |
dc.contributor.author | Le, SY | en_US |
dc.contributor.author | Yeung, ML | en_US |
dc.contributor.author | Jeang, KT | en_US |
dc.date.accessioned | 2012-08-08T08:50:09Z | - |
dc.date.available | 2012-08-08T08:50:09Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Methods In Molecular Biology (Clifton, N.J.), 2006, v. 342, p. 241-253 | en_US |
dc.identifier.issn | 1064-3745 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/157461 | - |
dc.description.abstract | Initially reported for Caenorhabditis elegans, microRNA (miRNA) has been shown to regulate gene expression in plants, flies, and mammals . Here, we report on our approaches to investigate the role of miRNAs in human immunodeficiency virus (HIV)-1 infection. Using computer-directed foldings, we first identify potential sequences in HIV-1 that putatively encode miRNAs. Subsequently, we use Northern blotting of RNAs isolated from HIV-infected cells to confirm expression of predicted miRNA sequences. Finally, we use a scanning algorithm to search 3' untranslated regions (UTRs) of human messenger RNAs (mRNAs) in the attempt to predict potential sites targeted by HIV-1 miRNAs. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Methods in molecular biology (Clifton, N.J.) | en_US |
dc.subject.mesh | Algorithms | en_US |
dc.subject.mesh | Computational Biology - Methods | en_US |
dc.subject.mesh | Hiv Infections - Virology | en_US |
dc.subject.mesh | Hiv-1 - Genetics | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Micrornas - Chemistry - Genetics - Metabolism | en_US |
dc.subject.mesh | Microarray Analysis - Methods | en_US |
dc.title | MicroRNAs in human immunodeficiency virus-1 infection. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Yeung, ML:pmlyeung@hku.hk | en_US |
dc.identifier.authority | Yeung, ML=rp01402 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 16957379 | - |
dc.identifier.scopus | eid_2-s2.0-33749048265 | en_US |
dc.identifier.volume | 342 | en_US |
dc.identifier.spage | 241 | en_US |
dc.identifier.epage | 253 | en_US |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Bennasser, Y=8335747500 | en_US |
dc.identifier.scopusauthorid | Le, SY=7006184376 | en_US |
dc.identifier.scopusauthorid | Yeung, ML=8350940900 | en_US |
dc.identifier.scopusauthorid | Jeang, KT=7004824803 | en_US |
dc.identifier.issnl | 1064-3745 | - |