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Article: Biotin biosynthesis; structure and mechanism of kara synthase from E. Coli

TitleBiotin biosynthesis; structure and mechanism of kara synthase from E. Coli
Authors
Campopiano, DJAlexeev, DAlexeeva, MWebster, SPWatt, RMSawyer, LBaxter, RL
Issue Date1997
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
Faseb Journal, 1997, v. 11 n. 9, p. A891 How to Cite?
AbstractIn E. co the first step in biotin biosynthesis is the condensation of pimeloyl CoA with alanine to form 8amino-7-oxononanoic acid (AON). This reaction is catalysed by the pyridoxal phosphate-dependent enzyme AON synthase (AONS, bioF gene product). We have cloned, overexpressed and purified the enzyme and characterised the enzyme using a variety of spectroscopic techniques. We have now determined the structure of the enzyme to 1.9A using X-ray crystallography. We present details of the structure and mechanism of AONS, the first such analysis of an enzyme from the type II PLP family.
Persistent Identifierhttp://hdl.handle.net/10722/154431
ISSN
0892-6638
2021 Impact Factor: 5.834
2020 SCImago Journal Rankings: 1.709

 

DC FieldValueLanguage
dc.contributor.authorCampopiano, DJen_US
dc.contributor.authorAlexeev, Den_US
dc.contributor.authorAlexeeva, Men_US
dc.contributor.authorWebster, SPen_US
dc.contributor.authorWatt, RMen_US
dc.contributor.authorSawyer, Len_US
dc.contributor.authorBaxter, RLen_US
dc.date.accessioned2012-08-08T08:25:17Z-
dc.date.available2012-08-08T08:25:17Z-
dc.date.issued1997en_US
dc.identifier.citationFaseb Journal, 1997, v. 11 n. 9, p. A891en_US
dc.identifier.issn0892-6638en_US
dc.identifier.urihttp://hdl.handle.net/10722/154431-
dc.description.abstractIn E. co the first step in biotin biosynthesis is the condensation of pimeloyl CoA with alanine to form 8amino-7-oxononanoic acid (AON). This reaction is catalysed by the pyridoxal phosphate-dependent enzyme AON synthase (AONS, bioF gene product). We have cloned, overexpressed and purified the enzyme and characterised the enzyme using a variety of spectroscopic techniques. We have now determined the structure of the enzyme to 1.9A using X-ray crystallography. We present details of the structure and mechanism of AONS, the first such analysis of an enzyme from the type II PLP family.en_US
dc.languageengen_US
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_US
dc.relation.ispartofFASEB Journalen_US
dc.titleBiotin biosynthesis; structure and mechanism of kara synthase from E. Colien_US
dc.typeArticleen_US
dc.identifier.emailWatt, RM:rmwatt@hku.hken_US
dc.identifier.authorityWatt, RM=rp00043en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-33750110448en_US
dc.identifier.volume11en_US
dc.identifier.issue9en_US
dc.identifier.spageA891en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridCampopiano, DJ=6602680734en_US
dc.identifier.scopusauthoridAlexeev, D=6701529096en_US
dc.identifier.scopusauthoridAlexeeva, M=35607988700en_US
dc.identifier.scopusauthoridWebster, SP=7101874218en_US
dc.identifier.scopusauthoridWatt, RM=7102907536en_US
dc.identifier.scopusauthoridSawyer, L=7102690704en_US
dc.identifier.scopusauthoridBaxter, RL=26643014900en_US
dc.identifier.issnl0892-6638-

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