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Article: The interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replication

TitleThe interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replication
Authors
KeywordsDynein light chain
Flavivirus
Membrane protein
Recombinant subviral particles (RSPs)
Virus trafficking
Issue Date2011
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2011, v. 417 n. 2, p. 369-378 How to Cite?
AbstractThe role of the membrane protein (prM/M) in flavivirus life cycle remains unclear. Here, we identified a cellular interactor to the 40-residue-long ectodomain of prM/M (ectoM) using a yeast two-hybrid screen against a human cDNA library and GST pull-down assays. We showed that dynein light chain Tctex-1 interacts with the ectoM of dengue 1-4, West Nile, and Japanese encephalitis flaviviruses. No interaction was found with yellow fever and tick-borne flaviviruses. This interaction is highly specific since a single amino-acid change in the ectoM abrogates the interaction with Tctex-1. To understand the role of this interaction, silencing of Tctex-1 using siRNA was performed prior to infection. A significant decrease in progeny production was observed for dengue and West Nile viruses. Silencing Tctex-1 inhibited the production of recombinant dengue subviral particles (RSPs). Thus Tctex-1 may play a role in late stages of viral replication through its interaction with the membrane protein.
Persistent Identifierhttp://hdl.handle.net/10722/152637
ISSN
2023 Impact Factor: 2.8
2023 SCImago Journal Rankings: 0.838
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBrault, JBen_US
dc.contributor.authorKudelko, Men_US
dc.contributor.authorVidalain, POen_US
dc.contributor.authorTangy, Fen_US
dc.contributor.authorDespres, Pen_US
dc.contributor.authorPardigon, Nen_US
dc.date.accessioned2012-07-16T09:44:34Z-
dc.date.available2012-07-16T09:44:34Z-
dc.date.issued2011en_US
dc.identifier.citationVirology, 2011, v. 417 n. 2, p. 369-378en_US
dc.identifier.issn0042-6822-
dc.identifier.urihttp://hdl.handle.net/10722/152637-
dc.description.abstractThe role of the membrane protein (prM/M) in flavivirus life cycle remains unclear. Here, we identified a cellular interactor to the 40-residue-long ectodomain of prM/M (ectoM) using a yeast two-hybrid screen against a human cDNA library and GST pull-down assays. We showed that dynein light chain Tctex-1 interacts with the ectoM of dengue 1-4, West Nile, and Japanese encephalitis flaviviruses. No interaction was found with yellow fever and tick-borne flaviviruses. This interaction is highly specific since a single amino-acid change in the ectoM abrogates the interaction with Tctex-1. To understand the role of this interaction, silencing of Tctex-1 using siRNA was performed prior to infection. A significant decrease in progeny production was observed for dengue and West Nile viruses. Silencing Tctex-1 inhibited the production of recombinant dengue subviral particles (RSPs). Thus Tctex-1 may play a role in late stages of viral replication through its interaction with the membrane protein.-
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro-
dc.relation.ispartofVirologyen_US
dc.subjectDynein light chain-
dc.subjectFlavivirus-
dc.subjectMembrane protein-
dc.subjectRecombinant subviral particles (RSPs)-
dc.subjectVirus trafficking-
dc.subject.meshDengue Virus - physiology-
dc.subject.meshDyneins - genetics - metabolism-
dc.subject.meshEncephalitis Virus, Japanese - physiology-
dc.subject.meshProtein Interaction Mapping-
dc.subject.meshViral Envelope Proteins - metabolism-
dc.titleThe interaction of flavivirus M protein with light chain Tctex-1 of human dynein plays a role in late stages of virus replicationen_US
dc.typeArticleen_US
dc.identifier.emailKudelko, M: kudelko@hku.hken_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.virol.2011.06.022-
dc.identifier.pmid21767858-
dc.identifier.scopuseid_2-s2.0-80051942967-
dc.identifier.hkuros201707en_US
dc.identifier.volume417en_US
dc.identifier.issue2en_US
dc.identifier.spage369en_US
dc.identifier.epage378en_US
dc.identifier.isiWOS:000294578600016-
dc.publisher.placeUnited States-
dc.identifier.citeulike9631080-
dc.identifier.issnl0042-6822-

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