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Article: Quantification of Saccharomyces cerevisiae viability using BacLight.

TitleQuantification of Saccharomyces cerevisiae viability using BacLight.
Authors
Zhang, TFang, HH
Issue Date2004
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492
Citation
Biotechnology Letters, 2004, v. 26 n. 12, p. 989-992 How to Cite?
DOI: http://dx.doi.org/10.1023/B:BILE.0000030045.16713.19
AbstractYeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.
Persistent Identifierhttp://hdl.handle.net/10722/150287
ISSN
0141-5492
2023 Impact Factor: 2.0
2023 SCImago Journal Rankings: 0.519
ISI Accession Number ID
WOS:000221806100008

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ten_US
dc.contributor.authorFang, HHen_US
dc.date.accessioned2012-06-26T06:03:02Z-
dc.date.available2012-06-26T06:03:02Z-
dc.date.issued2004en_US
dc.identifier.citationBiotechnology Letters, 2004, v. 26 n. 12, p. 989-992en_US
dc.identifier.issn0141-5492en_US
dc.identifier.urihttp://hdl.handle.net/10722/150287-
dc.description.abstractYeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.en_US
dc.languageengen_US
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492en_US
dc.relation.ispartofBiotechnology lettersen_US
dc.subject.meshCell Culture Techniques - Instrumentation - Methodsen_US
dc.subject.meshCell Survival - Physiologyen_US
dc.subject.meshColony Count, Microbial - Instrumentation - Methodsen_US
dc.subject.meshFlow Cytometry - Instrumentation - Methodsen_US
dc.subject.meshFluorescent Dyesen_US
dc.subject.meshGreen Fluorescent Proteinsen_US
dc.subject.meshMicroscopy, Confocal - Instrumentation - Methodsen_US
dc.subject.meshMicroscopy, Fluorescence - Instrumentation - Methodsen_US
dc.subject.meshPropidiumen_US
dc.subject.meshReagent Kits, Diagnosticen_US
dc.subject.meshSaccharomyces Cerevisiae - Cytology - Isolation & Purification - Physiologyen_US
dc.titleQuantification of Saccharomyces cerevisiae viability using BacLight.en_US
dc.typeArticleen_US
dc.identifier.emailZhang, T:zhangt@hkucc.hku.hken_US
dc.identifier.emailFang, HH:hrechef@hkucc.hku.hken_US
dc.identifier.authorityZhang, T=rp00211en_US
dc.identifier.authorityFang, HH=rp00115en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1023/B:BILE.0000030045.16713.19-
dc.identifier.pmid15269525-
dc.identifier.scopuseid_2-s2.0-16644393440en_US
dc.identifier.hkuros93239-
dc.identifier.volume26en_US
dc.identifier.issue12en_US
dc.identifier.spage989en_US
dc.identifier.epage992en_US
dc.identifier.isiWOS:000221806100008-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridZhang, T=24470677400en_US
dc.identifier.scopusauthoridFang, HH=7402542625en_US
dc.identifier.issnl0141-5492-

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