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Article: A lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean

TitleA lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Bean
Authors
Issue Date2010
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau
Citation
Journal Of Agricultural And Food Chemistry, 2010, v. 58 n. 4, p. 2221-2229 How to Cite?
AbstractLectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy. © 2010 American Chemical Society Phaseolus vulgaris cv. Extralong Autumn Purple Bean, purification, lectin, anti-HIV, antitumor, nasopharyngeal carcinoma, nitric oxide, apoptotic body.
Persistent Identifierhttp://hdl.handle.net/10722/149733
ISSN
2023 Impact Factor: 5.7
2023 SCImago Journal Rankings: 1.114
ISI Accession Number ID
Funding AgencyGrant Number
Chinese University of Hong Kong
Funding Information:

We thank Helen Hai-iing Liu, Yuan Zhao, and Wai-hung Lau from the Chinese University of Hong Kong For their kind support of this work.

References

 

DC FieldValueLanguage
dc.contributor.authorFang, EFen_US
dc.contributor.authorLin, Pen_US
dc.contributor.authorWong, JHen_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorNg, TBen_US
dc.date.accessioned2012-06-26T05:57:47Z-
dc.date.available2012-06-26T05:57:47Z-
dc.date.issued2010en_US
dc.identifier.citationJournal Of Agricultural And Food Chemistry, 2010, v. 58 n. 4, p. 2221-2229en_US
dc.identifier.issn0021-8561en_US
dc.identifier.urihttp://hdl.handle.net/10722/149733-
dc.description.abstractLectins/hemagglutinins are a class of sugar-binding proteins which agglutinate cells and/or precipitate glycoconjugates. They occur widely in plants but manifest significant differences in activities, which means only a few of them own exploitable potentials. The objective of this study was to find and characterize a multifunctional plant lectin with high potential values in food chemistry and medicine. A 60-kDa lectin from Phaseolus vulgaris L. cv. Extralong Autumn Purple Bean (EAPL) was purified by liquid chromatography, and the sequence of its first 20 N-terminal amino acids was ANEIYFSFQRFNETNLILQR. It was galactose-specific and manifested hemagglutinating activity toward erythrocytes of rabbit, rat, mouse, and human ABO blood types. EAPL manifested anti-HIV1-RT activity, and it could inhibit the proliferation of human tumor cells by inducing the production of apoptotic bodies. The nitric oxide-inducing activity of EAPL may find application in tumor therapy. © 2010 American Chemical Society Phaseolus vulgaris cv. Extralong Autumn Purple Bean, purification, lectin, anti-HIV, antitumor, nasopharyngeal carcinoma, nitric oxide, apoptotic body.en_US
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcauen_US
dc.relation.ispartofJournal of Agricultural and Food Chemistryen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Division - Drug Effectsen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshEnzyme Inhibitors - Chemistry - Isolation & Purification - Pharmacologyen_US
dc.subject.meshErythrocytes - Drug Effects - Physiologyen_US
dc.subject.meshFabaceae - Chemistryen_US
dc.subject.meshHiv Reverse Transcriptase - Isolation & Purificationen_US
dc.subject.meshHiv-1 - Drug Effects - Enzymologyen_US
dc.subject.meshHemagglutination - Drug Effectsen_US
dc.subject.meshHumansen_US
dc.subject.meshLectins - Isolation & Purification - Pharmacologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNasopharyngeal Neoplasmsen_US
dc.subject.meshNitric Oxide Synthase Type Ii - Drug Effects - Geneticsen_US
dc.subject.meshPhaseolus - Chemistryen_US
dc.subject.meshRna, Messenger - Geneticsen_US
dc.subject.meshRabbitsen_US
dc.subject.meshRatsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSeasonsen_US
dc.titleA lectin with anti-HIV-1 reverse transcriptase, antitumor, and nitric oxide inducing activities from seeds of Phaseolus vulgaris cv. Extralong Autumn Purple Beanen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/jf903964uen_US
dc.identifier.pmid20095617-
dc.identifier.scopuseid_2-s2.0-77249112249en_US
dc.identifier.hkuros182827-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77249112249&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume58en_US
dc.identifier.issue4en_US
dc.identifier.spage2221en_US
dc.identifier.epage2229en_US
dc.identifier.isiWOS:000274530100025-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridFang, EF=35386218400en_US
dc.identifier.scopusauthoridLin, P=55228034700en_US
dc.identifier.scopusauthoridWong, JH=34874358200en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridNg, TB=35311803300en_US
dc.identifier.issnl0021-8561-

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