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Article: Specificity of different isoforms of protein phosphatase-2A and protein phosphatase-2C studied using site-directed mutagenesis of HMG-CoA reductase

TitleSpecificity of different isoforms of protein phosphatase-2A and protein phosphatase-2C studied using site-directed mutagenesis of HMG-CoA reductase
Authors
KeywordsAMP-activated protein kinase
Dephosphorylation
HMG-CoA reductase
Protein phosphatase-2A
Protein phosphatase-2C
Site-directed mutagenesis
Issue Date1997
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 1997, v. 411 n. 2-3, p. 265-268 How to Cite?
AbstractWe have expressed the catalytic domain of Chinese hamster HMG-CoA reductase, and 13 point mutations involving the region around the single phosphorylation site for AMP-activated protein kinase. After phosphorylation, these were used to test the specificity of isoforms of protein phosphatase-2A [bovine PP2A(C) (catalytic subunit) and PP2A1 (ABC heterotrimer)] and protein phosphatase-2C (human α; mouse α, β1, β2, β3, β4, β5). PP2A1 had > 50-fold higher activity for HMG-CoA reductase variants than PP2A(C), but their relative selectivity for different variants was similar. Although the specificities of PP2A and PP2C were distinct, no dramatic differences in selectivity were observed between different PP2C isoforms.
Persistent Identifierhttp://hdl.handle.net/10722/149570
ISSN
2023 Impact Factor: 3.0
2023 SCImago Journal Rankings: 1.208
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPang Ching, Yen_US
dc.contributor.authorKobayashi, Ten_US
dc.contributor.authorTamura, Sen_US
dc.contributor.authorGrahame Hardie, Den_US
dc.date.accessioned2012-06-26T05:55:24Z-
dc.date.available2012-06-26T05:55:24Z-
dc.date.issued1997en_US
dc.identifier.citationFebs Letters, 1997, v. 411 n. 2-3, p. 265-268en_US
dc.identifier.issn0014-5793en_US
dc.identifier.urihttp://hdl.handle.net/10722/149570-
dc.description.abstractWe have expressed the catalytic domain of Chinese hamster HMG-CoA reductase, and 13 point mutations involving the region around the single phosphorylation site for AMP-activated protein kinase. After phosphorylation, these were used to test the specificity of isoforms of protein phosphatase-2A [bovine PP2A(C) (catalytic subunit) and PP2A1 (ABC heterotrimer)] and protein phosphatase-2C (human α; mouse α, β1, β2, β3, β4, β5). PP2A1 had > 50-fold higher activity for HMG-CoA reductase variants than PP2A(C), but their relative selectivity for different variants was similar. Although the specificities of PP2A and PP2C were distinct, no dramatic differences in selectivity were observed between different PP2C isoforms.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_US
dc.relation.ispartofFEBS Lettersen_US
dc.subjectAMP-activated protein kinase-
dc.subjectDephosphorylation-
dc.subjectHMG-CoA reductase-
dc.subjectProtein phosphatase-2A-
dc.subjectProtein phosphatase-2C-
dc.subjectSite-directed mutagenesis-
dc.subject.meshAmp-Activated Protein Kinasesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCho Cellsen_US
dc.subject.meshCattleen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEscherichia Coli - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshHydroxymethylglutaryl Coa Reductases - Chemistry - Genetics - Metabolismen_US
dc.subject.meshIsoenzymes - Metabolismen_US
dc.subject.meshKineticsen_US
dc.subject.meshMiceen_US
dc.subject.meshMultienzyme Complexes - Metabolismen_US
dc.subject.meshMutagenesis, Site-Directeden_US
dc.subject.meshPhosphoprotein Phosphatases - Chemistry - Metabolismen_US
dc.subject.meshPhosphorylationen_US
dc.subject.meshPoint Mutationen_US
dc.subject.meshProtein Kinases - Metabolismen_US
dc.subject.meshProtein Phosphatase 2en_US
dc.subject.meshProtein-Serine-Threonine Kinasesen_US
dc.subject.meshRecombinant Proteins - Metabolismen_US
dc.subject.meshSaccharomyces Cerevisiae Proteinsen_US
dc.subject.meshSubstrate Specificityen_US
dc.titleSpecificity of different isoforms of protein phosphatase-2A and protein phosphatase-2C studied using site-directed mutagenesis of HMG-CoA reductaseen_US
dc.typeArticleen_US
dc.identifier.emailPang Ching, Y:ypching@hku.hken_US
dc.identifier.authorityPang Ching, Y=rp00469en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0014-5793(97)00712-6en_US
dc.identifier.pmid9271218-
dc.identifier.scopuseid_2-s2.0-0031567606en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031567606&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume411en_US
dc.identifier.issue2-3en_US
dc.identifier.spage265en_US
dc.identifier.epage268en_US
dc.identifier.isiWOS:A1997XN02400023-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridPang Ching, Y=7005431277en_US
dc.identifier.scopusauthoridKobayashi, T=7406708543en_US
dc.identifier.scopusauthoridTamura, S=35399280000en_US
dc.identifier.scopusauthoridGrahame Hardie, D=6602300838en_US
dc.identifier.issnl0014-5793-

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