File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1002/pros.2990140207
- Scopus: eid_2-s2.0-0024389331
- PMID: 2710691
- WOS: WOS:A1989U375900006
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Cytochemical characterization of cuprolinic blue-stained proteoglycans in the epithelial-stromal interface of the guinea pig lateral prostate
Title | Cytochemical characterization of cuprolinic blue-stained proteoglycans in the epithelial-stromal interface of the guinea pig lateral prostate |
---|---|
Authors | |
Keywords | chondroitin sulfate dermatan sulfate heparan sulfate |
Issue Date | 1989 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304 |
Citation | Prostate, 1989, v. 14 n. 2, p. 133-145 How to Cite? |
Abstract | Three types (T1, T2, T3) of proetoglycan (PG) filaments, as demonstrated by cuprolinic blue (CB) under critical electrolyte concentration method in the epithelial-stromal interface of the guinea pig lateral prostate, were characterized cytochemically by using a number of glycosaminoglycan(GAG)-degrading enzymes and nitrous acid. The results showed that T1 filaments located in basement membranes of the epithelium, endothelium, and smooth muscle cells, were removed by nitrous acid, heparitinase, and pronase but resistant to chondroitinase (Ch)-ABC and Ch-AC, heparinase, neuraminidase, and Streptomyces (S) hyaluronidase. The T1 filaments, therefore, contain heparan sulfate. The T2 filaments closely linked to collagen fibrils were removed by Ch-ABC, Ch-ABC plus S-hyaluronidase, and pronase but were resistant to nitrous acid, heparitinase, heparinase, neuraminidase, and S-hyaluronidase. These show that T2 filaments are rich in dermatan sulfate. The T3 filaments in the interstitial spaces and on the surface of fibroblasts were removed by Ch-ABC, Ch-AC, and pronase but were resistant to heparitinase, heparinase, hyaluronidase, neuraminidase, and nitrous acid. They are, therefore, rich in chondroitin sulfate. |
Persistent Identifier | http://hdl.handle.net/10722/149491 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 1.032 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Chan, L | en_US |
dc.contributor.author | Wong, YC | en_US |
dc.date.accessioned | 2012-06-26T05:54:29Z | - |
dc.date.available | 2012-06-26T05:54:29Z | - |
dc.date.issued | 1989 | en_US |
dc.identifier.citation | Prostate, 1989, v. 14 n. 2, p. 133-145 | en_US |
dc.identifier.issn | 0270-4137 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/149491 | - |
dc.description.abstract | Three types (T1, T2, T3) of proetoglycan (PG) filaments, as demonstrated by cuprolinic blue (CB) under critical electrolyte concentration method in the epithelial-stromal interface of the guinea pig lateral prostate, were characterized cytochemically by using a number of glycosaminoglycan(GAG)-degrading enzymes and nitrous acid. The results showed that T1 filaments located in basement membranes of the epithelium, endothelium, and smooth muscle cells, were removed by nitrous acid, heparitinase, and pronase but resistant to chondroitinase (Ch)-ABC and Ch-AC, heparinase, neuraminidase, and Streptomyces (S) hyaluronidase. The T1 filaments, therefore, contain heparan sulfate. The T2 filaments closely linked to collagen fibrils were removed by Ch-ABC, Ch-ABC plus S-hyaluronidase, and pronase but were resistant to nitrous acid, heparitinase, heparinase, neuraminidase, and S-hyaluronidase. These show that T2 filaments are rich in dermatan sulfate. The T3 filaments in the interstitial spaces and on the surface of fibroblasts were removed by Ch-ABC, Ch-AC, and pronase but were resistant to heparitinase, heparinase, hyaluronidase, neuraminidase, and nitrous acid. They are, therefore, rich in chondroitin sulfate. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304 | en_US |
dc.relation.ispartof | Prostate | en_US |
dc.subject | chondroitin sulfate | - |
dc.subject | dermatan sulfate | - |
dc.subject | heparan sulfate | - |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Chondroitin Sulfate Proteoglycans - Analysis | en_US |
dc.subject.mesh | Epithelium | en_US |
dc.subject.mesh | Glycosaminoglycans - Analysis | en_US |
dc.subject.mesh | Guinea Pigs | en_US |
dc.subject.mesh | Indoles - Diagnostic Use | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Organometallic Compounds | en_US |
dc.subject.mesh | Prostate - Analysis - Ultrastructure | en_US |
dc.subject.mesh | Proteoglycans - Analysis | en_US |
dc.title | Cytochemical characterization of cuprolinic blue-stained proteoglycans in the epithelial-stromal interface of the guinea pig lateral prostate | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wong, YC:ycwong@hkucc.hku.hk | en_US |
dc.identifier.authority | Wong, YC=rp00316 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/pros.2990140207 | - |
dc.identifier.pmid | 2710691 | - |
dc.identifier.scopus | eid_2-s2.0-0024389331 | en_US |
dc.identifier.volume | 14 | en_US |
dc.identifier.issue | 2 | en_US |
dc.identifier.spage | 133 | en_US |
dc.identifier.epage | 145 | en_US |
dc.identifier.isi | WOS:A1989U375900006 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Chan, L=35336076700 | en_US |
dc.identifier.scopusauthorid | Wong, YC=7403041798 | en_US |
dc.identifier.issnl | 0270-4137 | - |