Interactions between compound and normal eye projections in dually innervated tectum: A study of optic nerve regeneration in Xenopus

Abstract

Right compound eyes were formed in Xenopus embryos at tailbud stages by the fusion of two nasal (NN), two temporal (TT) or two ventral (VV) halves. The left eye was kept intact. Two to four weeks after metamorphosis the optic nerve from the intact eye was severed to induce bilateral optic nerve regeneration. The contralateral retinotectal projections from the compound eye and the induced ipsilateral projections from the intact eye to the same (dually innervated tectum were studied by [3H]proline autoradiography and visuotectal mapping from 3 to 6 months after the postmetamorphic surgery. The results showed that the NN, TT and VV projections, in the presence of optic fibres from the intact eye failed to spread across the whole extent of the dually innervated tectum. Unexpectedly the bulk of the regenerating projection from the intact eye was confined to the previously uninnervated parts of the dually innervated tecta, the caudomedial region in TT, the rostrolateral region in NN and the lateral region in VV eye animals. The partial segregation of the two populations of optic fibres in the dually innervated tectum has been taken as a further indication of the role of fibre-fibre and fibre-tectum interactions in retinotectal map formation.