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- Publisher Website: 10.1177/34.3.2419389
- Scopus: eid_2-s2.0-0022624036
- PMID: 2419389
- WOS: WOS:A1986A094000002
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Article: Localization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies
Title | Localization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies |
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Authors | |
Issue Date | 1986 |
Publisher | Histochemical Society. The Journal's web site is located at http://intl.jhc.org |
Citation | Journal of Histochemistry and Cytochemistry, 1986, v. 34 n. 3, p. 287-292 How to Cite? |
Abstract | Mouse monoclonal and rabbit polyclonal antibodies to human pituitary hormones were applied together to sections of normal and neoplastic human pituitary tissue. Binding sites were revealed with species-specific immune reagents combined with various enzymes (peroxidase, alkaline phosphatase, and beta-D-galactosidase). The enzymes were developed separately to give differently colored end-products. Where two hormones were present in the same cell, a mixed color was produced. Up to four hormones could be immunostained in a single section. Multiple immunoenzymatic staining has great potential for the analysis of plural antigen production by single cells and relationships between cells producing different antigens. |
Persistent Identifier | http://hdl.handle.net/10722/148858 |
ISSN | 2023 Impact Factor: 1.9 2023 SCImago Journal Rankings: 1.177 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Van Noorden, S | - |
dc.contributor.author | Stuart, MC | - |
dc.contributor.author | Cheung, A | - |
dc.contributor.author | Adams, EF | - |
dc.contributor.author | Polak, JM | - |
dc.date.accessioned | 2012-06-20T01:16:45Z | - |
dc.date.available | 2012-06-20T01:16:45Z | - |
dc.date.issued | 1986 | - |
dc.identifier.citation | Journal of Histochemistry and Cytochemistry, 1986, v. 34 n. 3, p. 287-292 | - |
dc.identifier.issn | 0022-1554 | - |
dc.identifier.uri | http://hdl.handle.net/10722/148858 | - |
dc.description.abstract | Mouse monoclonal and rabbit polyclonal antibodies to human pituitary hormones were applied together to sections of normal and neoplastic human pituitary tissue. Binding sites were revealed with species-specific immune reagents combined with various enzymes (peroxidase, alkaline phosphatase, and beta-D-galactosidase). The enzymes were developed separately to give differently colored end-products. Where two hormones were present in the same cell, a mixed color was produced. Up to four hormones could be immunostained in a single section. Multiple immunoenzymatic staining has great potential for the analysis of plural antigen production by single cells and relationships between cells producing different antigens. | - |
dc.language | eng | - |
dc.publisher | Histochemical Society. The Journal's web site is located at http://intl.jhc.org | - |
dc.relation.ispartof | Journal of Histochemistry and Cytochemistry | - |
dc.subject.mesh | Antibodies - diagnostic use | - |
dc.subject.mesh | Antibodies, Monoclonal - diagnostic use | - |
dc.subject.mesh | Immunoenzyme Techniques | - |
dc.subject.mesh | Pituitary Hormones - metabolism | - |
dc.subject.mesh | Pituitary Neoplasms - metabolism - pathology | - |
dc.title | Localization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies | en_US |
dc.type | Article | en_US |
dc.identifier.email | Cheung, A: lmcheung@hkucc.hku.hk | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1177/34.3.2419389 | - |
dc.identifier.pmid | 2419389 | - |
dc.identifier.scopus | eid_2-s2.0-0022624036 | - |
dc.identifier.volume | 34 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 287 | - |
dc.identifier.epage | 292 | - |
dc.identifier.isi | WOS:A1986A094000002 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-1554 | - |