File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The initial and subsequent inflammatory events during calcium oxalate lithiasis

TitleThe initial and subsequent inflammatory events during calcium oxalate lithiasis
Authors
KeywordsCalcium oxalate
Cell injury
Endocytosis
Hyaluronan
Proximal tubular cells
Renal stone
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/cca
Citation
Clinica Chimica Acta, 2010, v. 411 n. 1-16, p. 1018-1026 How to Cite?
AbstractBackground: Crystallization is believed to be the initiation step of urolithiasis, even though it is unknown where inside the nephron the first crystal nucleation occurs. Methods: Direct nucleation of calcium oxalate and subsequent events including crystal retention, cellular damage, endocytosis, and hyaluronan (HA) expression, were tested in a two-compartment culture system with intact human proximal tubular HK-2 cell monolayer. Results: Calcium oxalate dihydrate (COD) was nucleated and bound onto the apical surface of the HK-2 cells under hypercalciuric and hyperoxaluric conditions. These cells displayed mild cellular damage and internalized some of the adhered crystals within 18. h post-COD-exposure, as revealed by electron microscopy. Prolonged incubation in complete medium caused significant damage to disrupt the monolayer integrity. Furthermore, hyaluronan disaccharides were detected in the harvested media, and were associated with HAS-3 mRNA expression. Conclusion: Human proximal cells were able to internalize COD crystals which nucleated directly onto the apical surface, subsequently triggering cellular damage and HAS-3 specific hyaluronan synthesis as an inflammatory response. The proximal tubule cells here demonstrate that it plays an important role in facilitating urolithiasis via endocytosis and creating an inflammatory environment whereby free hyaluronan in tubular fluid can act as crystal-binding molecule at the later segments of distal and collecting tubules. © 2010 Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/147619
ISSN
2023 Impact Factor: 3.2
2023 SCImago Journal Rankings: 1.016
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants Council (RGC) at the Hong Kong Polytechnic University5375/04M
Funding Information:

The authors thank Mr. Michael Chiang (Department of Biology and Chemistry, City University of Hong Kong) who provided valuable technical supports on electronic microscopic analysis. This study was funded by the Hong Kong Research Grants Council (RGC-PolyU, 5375/04M) at the Hong Kong Polytechnic University and account no. 7002376 to the City University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorYuen, JWMen_US
dc.contributor.authorGohel, MDIen_US
dc.contributor.authorPoon, NWen_US
dc.contributor.authorShum, DKYen_US
dc.contributor.authorTam, PCen_US
dc.contributor.authorAu, DWTen_US
dc.date.accessioned2012-05-29T06:05:01Z-
dc.date.available2012-05-29T06:05:01Z-
dc.date.issued2010en_US
dc.identifier.citationClinica Chimica Acta, 2010, v. 411 n. 1-16, p. 1018-1026en_US
dc.identifier.issn0009-8981en_US
dc.identifier.urihttp://hdl.handle.net/10722/147619-
dc.description.abstractBackground: Crystallization is believed to be the initiation step of urolithiasis, even though it is unknown where inside the nephron the first crystal nucleation occurs. Methods: Direct nucleation of calcium oxalate and subsequent events including crystal retention, cellular damage, endocytosis, and hyaluronan (HA) expression, were tested in a two-compartment culture system with intact human proximal tubular HK-2 cell monolayer. Results: Calcium oxalate dihydrate (COD) was nucleated and bound onto the apical surface of the HK-2 cells under hypercalciuric and hyperoxaluric conditions. These cells displayed mild cellular damage and internalized some of the adhered crystals within 18. h post-COD-exposure, as revealed by electron microscopy. Prolonged incubation in complete medium caused significant damage to disrupt the monolayer integrity. Furthermore, hyaluronan disaccharides were detected in the harvested media, and were associated with HAS-3 mRNA expression. Conclusion: Human proximal cells were able to internalize COD crystals which nucleated directly onto the apical surface, subsequently triggering cellular damage and HAS-3 specific hyaluronan synthesis as an inflammatory response. The proximal tubule cells here demonstrate that it plays an important role in facilitating urolithiasis via endocytosis and creating an inflammatory environment whereby free hyaluronan in tubular fluid can act as crystal-binding molecule at the later segments of distal and collecting tubules. © 2010 Elsevier B.V.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ccaen_US
dc.relation.ispartofClinica Chimica Actaen_US
dc.subjectCalcium oxalate-
dc.subjectCell injury-
dc.subjectEndocytosis-
dc.subjectHyaluronan-
dc.subjectProximal tubular cells-
dc.subjectRenal stone-
dc.subject.meshBiological Transporten_US
dc.subject.meshCalcium - Pharmacologyen_US
dc.subject.meshCalcium Oxalate - Chemistry - Metabolism - Pharmacologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshEpithelial Cells - Drug Effects - Metabolism - Pathologyen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshGlucuronosyltransferase - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshInflammation - Genetics - Metabolism - Pathologyen_US
dc.subject.meshKidney Tubules, Proximal - Pathologyen_US
dc.subject.meshLithiasis - Genetics - Metabolism - Pathologyen_US
dc.titleThe initial and subsequent inflammatory events during calcium oxalate lithiasisen_US
dc.typeArticleen_US
dc.identifier.emailShum, DKY:shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.cca.2010.03.015en_US
dc.identifier.pmid20347754-
dc.identifier.scopuseid_2-s2.0-77953326723en_US
dc.identifier.hkuros172702-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77953326723&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume411en_US
dc.identifier.issue1-16en_US
dc.identifier.spage1018en_US
dc.identifier.epage1026en_US
dc.identifier.isiWOS:000279299100003-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridYuen, JWM=7102620486en_US
dc.identifier.scopusauthoridGohel, MDI=7004532182en_US
dc.identifier.scopusauthoridPoon, NW=36497383300en_US
dc.identifier.scopusauthoridShum, DKY=7004824447en_US
dc.identifier.scopusauthoridTam, PC=7202539419en_US
dc.identifier.scopusauthoridAu, DWT=7004909228en_US
dc.identifier.citeulike6958918-
dc.identifier.issnl0009-8981-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats