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- Publisher Website: 10.1002/cbic.200800491
- Scopus: eid_2-s2.0-59649092194
- PMID: 19031435
- WOS: WOS:000261876900021
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Article: Differential inhibitory activities and stabilisation of DNA aptamers against the SARS coronavirus helicase.
Title | Differential inhibitory activities and stabilisation of DNA aptamers against the SARS coronavirus helicase. |
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Authors | |
Issue Date | 2008 |
Citation | Chembiochem : A European Journal Of Chemical Biology, 2008, v. 9 n. 18, p. 3037-3045 How to Cite? |
Abstract | The helicase from severe acute respiratory syndrome coronavirus (SARS-CoV) possesses NTPase, duplex RNA/DNA-unwinding and RNA-capping activities that are essential for viral replication and proliferation. Here, we have isolated DNA aptamers against the SARS-CoV helicase from a combinatorial DNA library. These aptamers show two distinct classes of secondary structure, G-quadruplex and non-G-quadruplex, as shown by circular dichroism and gel electrophoresis. All of the aptamers that were selected stimulated ATPase activity of the SARS-CoV helicase with low-nanomolar apparent K(m) values. Intriguingly, only the non-G-quadruplex aptamers showed specific inhibition of helicase activities, whereas the G-quadruplex aptamers did not inhibit helicase activities. The non-G-quadruplex aptamer with the strongest inhibitory potency was modified at the 3'-end with biotin or inverted thymidine, and the modification increased its stability in serum, particularly for the inverted thymidine modification. Structural diversity in selection coupled to post-selection stabilisation has provided new insights into the aptamers that were selected for a helicase target. These aptamers are being further developed to inhibit SARS-CoV replication. |
Persistent Identifier | http://hdl.handle.net/10722/147596 |
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.809 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Shum, KT | en_US |
dc.contributor.author | Tanner, JA | en_US |
dc.date.accessioned | 2012-05-29T06:04:50Z | - |
dc.date.available | 2012-05-29T06:04:50Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Chembiochem : A European Journal Of Chemical Biology, 2008, v. 9 n. 18, p. 3037-3045 | en_US |
dc.identifier.issn | 1439-7633 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147596 | - |
dc.description.abstract | The helicase from severe acute respiratory syndrome coronavirus (SARS-CoV) possesses NTPase, duplex RNA/DNA-unwinding and RNA-capping activities that are essential for viral replication and proliferation. Here, we have isolated DNA aptamers against the SARS-CoV helicase from a combinatorial DNA library. These aptamers show two distinct classes of secondary structure, G-quadruplex and non-G-quadruplex, as shown by circular dichroism and gel electrophoresis. All of the aptamers that were selected stimulated ATPase activity of the SARS-CoV helicase with low-nanomolar apparent K(m) values. Intriguingly, only the non-G-quadruplex aptamers showed specific inhibition of helicase activities, whereas the G-quadruplex aptamers did not inhibit helicase activities. The non-G-quadruplex aptamer with the strongest inhibitory potency was modified at the 3'-end with biotin or inverted thymidine, and the modification increased its stability in serum, particularly for the inverted thymidine modification. Structural diversity in selection coupled to post-selection stabilisation has provided new insights into the aptamers that were selected for a helicase target. These aptamers are being further developed to inhibit SARS-CoV replication. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Chembiochem : a European journal of chemical biology | en_US |
dc.subject.mesh | Aptamers, Nucleotide - Chemistry - Pharmacology | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Dna Helicases - Antagonists & Inhibitors - Metabolism | en_US |
dc.subject.mesh | G-Quadruplexes | en_US |
dc.subject.mesh | Inhibitory Concentration 50 | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Sars Virus - Enzymology | en_US |
dc.subject.mesh | Viral Proteins - Antagonists & Inhibitors - Metabolism | en_US |
dc.title | Differential inhibitory activities and stabilisation of DNA aptamers against the SARS coronavirus helicase. | en_US |
dc.type | Article | en_US |
dc.identifier.email | Tanner, JA:jatanner@hku.hk | en_US |
dc.identifier.authority | Tanner, JA=rp00495 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/cbic.200800491 | - |
dc.identifier.pmid | 19031435 | - |
dc.identifier.scopus | eid_2-s2.0-59649092194 | en_US |
dc.identifier.hkuros | 152816 | - |
dc.identifier.volume | 9 | en_US |
dc.identifier.issue | 18 | en_US |
dc.identifier.spage | 3037 | en_US |
dc.identifier.epage | 3045 | en_US |
dc.identifier.eissn | 1439-7633 | - |
dc.identifier.isi | WOS:000261876900021 | - |
dc.identifier.scopusauthorid | Shum, KT=20436474600 | en_US |
dc.identifier.scopusauthorid | Tanner, JA=35513993000 | en_US |
dc.identifier.issnl | 1439-4227 | - |