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- PMID: 9124299
- WOS: WOS:A1997WJ63300023
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Article: Pertussis toxin, but not tyrosine kinase inhibitors, abolishes effects of U-50488H on [Ca2+](i) in myocytes
Title | Pertussis toxin, but not tyrosine kinase inhibitors, abolishes effects of U-50488H on [Ca2+](i) in myocytes |
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Authors | |
Keywords | κ-opioid receptor G protein phospholipase C tyrosine phosphorylation |
Issue Date | 1997 |
Publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/ |
Citation | American Journal of Physiology - Cell Physiology, 1997, v. 272 n. 2 41-2, p. C560-C564 How to Cite? |
Abstract | The effects of 10-5 M trans-3,4-dichloro-N-[2-(1- pyrrolidinyl)cyclohexyl]benzeacetamidel (U-50488H), a κ-opioid receptor agonist, on cytosolic Ca2+ concentration ([Ca2+](i)) and the [Ca2+](i) transient in quiescent and electrically stimulated rat ventricular myocytes, respectively, were determined after the cells had been pretreated with pertussis toxin (PTX) or a tyrosine kinase inhibitor (genistein or tyrphostin). The [Ca2+](i) was determined with a spectrofluorometric method, with fura 2 as Ca2+ indicator. U-50488H at 10-5 M itself induced a [Ca2+](i) transient in the quiescent cells but inhibited the [Ca2+](i) transient in electrically stimulated cells. The effects of 10-5 M U-50488H on [Ca2+](i), which were blocked by a selective K-opioid receptor antagonist, nor-binaltorphimine (10-6 M), were abolished after pretreatment with PTX (1 μg/ml) for 24 h, but not with genistein (10-4 M) or tyrphostin (5 x 10-5 M) for 30 min. 1-[6-[[(17b)-3-Methoxyestra-1,3,5(10)-trien-17- yl]amino]hexyl]-1H-pyrrole-2,5-dione (U-73122), an inhibitor of phospholipase C, at 10-5 M, but not its inactive structural isomer 1-[6-[[(17b)-3- methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-2,5-pyrrolidinedione (U- 73343), also blocked the Ca2+ responses to U-50488H. The results indicate that activation of phospholipase C on κ-opioid receptor stimulation is via PTX-sensitive G proteins but does not involve protein tyrosine phosphorylation. |
Persistent Identifier | http://hdl.handle.net/10722/147508 |
ISSN | 2023 Impact Factor: 5.0 2023 SCImago Journal Rankings: 1.711 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Sheng, JZ | en_US |
dc.contributor.author | Wong, NS | en_US |
dc.contributor.author | Wang, HX | en_US |
dc.contributor.author | Wong, TM | en_US |
dc.date.accessioned | 2012-05-29T06:04:13Z | - |
dc.date.available | 2012-05-29T06:04:13Z | - |
dc.date.issued | 1997 | en_US |
dc.identifier.citation | American Journal of Physiology - Cell Physiology, 1997, v. 272 n. 2 41-2, p. C560-C564 | en_US |
dc.identifier.issn | 0363-6143 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147508 | - |
dc.description.abstract | The effects of 10-5 M trans-3,4-dichloro-N-[2-(1- pyrrolidinyl)cyclohexyl]benzeacetamidel (U-50488H), a κ-opioid receptor agonist, on cytosolic Ca2+ concentration ([Ca2+](i)) and the [Ca2+](i) transient in quiescent and electrically stimulated rat ventricular myocytes, respectively, were determined after the cells had been pretreated with pertussis toxin (PTX) or a tyrosine kinase inhibitor (genistein or tyrphostin). The [Ca2+](i) was determined with a spectrofluorometric method, with fura 2 as Ca2+ indicator. U-50488H at 10-5 M itself induced a [Ca2+](i) transient in the quiescent cells but inhibited the [Ca2+](i) transient in electrically stimulated cells. The effects of 10-5 M U-50488H on [Ca2+](i), which were blocked by a selective K-opioid receptor antagonist, nor-binaltorphimine (10-6 M), were abolished after pretreatment with PTX (1 μg/ml) for 24 h, but not with genistein (10-4 M) or tyrphostin (5 x 10-5 M) for 30 min. 1-[6-[[(17b)-3-Methoxyestra-1,3,5(10)-trien-17- yl]amino]hexyl]-1H-pyrrole-2,5-dione (U-73122), an inhibitor of phospholipase C, at 10-5 M, but not its inactive structural isomer 1-[6-[[(17b)-3- methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-2,5-pyrrolidinedione (U- 73343), also blocked the Ca2+ responses to U-50488H. The results indicate that activation of phospholipase C on κ-opioid receptor stimulation is via PTX-sensitive G proteins but does not involve protein tyrosine phosphorylation. | en_US |
dc.language | eng | en_US |
dc.publisher | American Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/ | en_US |
dc.relation.ispartof | American Journal of Physiology - Cell Physiology | en_US |
dc.subject | κ-opioid receptor | - |
dc.subject | G protein | - |
dc.subject | phospholipase C | - |
dc.subject | tyrosine phosphorylation | - |
dc.subject.mesh | 3,4-Dichloro-N-Methyl-N-(2-(1-Pyrrolidinyl)-Cyclohexyl)-Benzeneacetamide, (Trans)-Isomer | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Calcium - Metabolism | en_US |
dc.subject.mesh | Cell Separation | en_US |
dc.subject.mesh | Cytosol - Metabolism | en_US |
dc.subject.mesh | Enzyme Inhibitors - Pharmacology | en_US |
dc.subject.mesh | Estrenes - Pharmacology | en_US |
dc.subject.mesh | Heart Ventricles | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Myocardium - Cytology - Metabolism | en_US |
dc.subject.mesh | Osmolar Concentration | en_US |
dc.subject.mesh | Pertussis Toxin | en_US |
dc.subject.mesh | Protein-Tyrosine Kinases - Antagonists & Inhibitors | en_US |
dc.subject.mesh | Pyrrolidines - Antagonists & Inhibitors - Pharmacology | en_US |
dc.subject.mesh | Pyrrolidinones - Pharmacology | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Sprague-Dawley | en_US |
dc.subject.mesh | Virulence Factors, Bordetella - Pharmacology | en_US |
dc.title | Pertussis toxin, but not tyrosine kinase inhibitors, abolishes effects of U-50488H on [Ca2+](i) in myocytes | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wong, NS:nswong@hkucc.hku.hk | en_US |
dc.identifier.authority | Wong, NS=rp00340 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 9124299 | - |
dc.identifier.scopus | eid_2-s2.0-16944367171 | en_US |
dc.identifier.hkuros | 25881 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-16944367171&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 272 | en_US |
dc.identifier.issue | 2 41-2 | en_US |
dc.identifier.spage | C560 | en_US |
dc.identifier.epage | C564 | en_US |
dc.identifier.isi | WOS:A1997WJ63300023 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Sheng, JZ=36846286500 | en_US |
dc.identifier.scopusauthorid | Wong, NS=7202836641 | en_US |
dc.identifier.scopusauthorid | Wang, HX=15119990000 | en_US |
dc.identifier.scopusauthorid | Wong, TM=7403531434 | en_US |
dc.identifier.issnl | 0363-6143 | - |