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Article: κ-opioid receptor agonist inhibits the cholera toxin-sensitive G protein in the heart

Titleκ-opioid receptor agonist inhibits the cholera toxin-sensitive G protein in the heart
Authors
KeywordsCholera toxin-sensitive G protein
Norepinephrine
Pertussis toxin-sensitive G protein
U50,488H
Ventricular myocyte
Issue Date2001
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.cardiovascularpharm.com/
Citation
Journal Of Cardiovascular Pharmacology, 2001, v. 38 n. 2, p. 232-239 How to Cite?
AbstractTo explore the signaling mechanisms of the negative modulation of β-adrenoceptors by κ-opioid receptors (κ-OR) in the heart, the possibility of the interaction at the level of G protein and receptor was determined. Cholera toxin, an activator of the stimulatory G protein (Gs), elevated electrically induced intracellular Ca2+ ([Ca2+]i) transients and induced ribosylation of the α-subunit of Gs (Gsα) in rat ventricular myocytes. The effects were significantly attenuated by U50,488H, a specific agonist of κ-OR, and were abolished by nor-binaltorphimine, a selective κ-OR antagonist. The content of Gsα, however, was not affected by U50,488H. Receptor binding experiments showed that neither Bmax nor Kd of the binding of [3H]CGP-12177, a β-adrenoceptor antagonist, was affected by U50,488H. The current study provides the first evidence that κ-OR stimulation inhibits the ribosylation of the α-subunit of the Gs protein, thus inhibiting the action of cholera toxin on the protein.
Persistent Identifierhttp://hdl.handle.net/10722/147461
ISSN
2023 Impact Factor: 2.6
2023 SCImago Journal Rankings: 0.610
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYu, XCen_US
dc.contributor.authorDiao, TMen_US
dc.contributor.authorPei, JMen_US
dc.contributor.authorZhang, WMen_US
dc.contributor.authorWong, NSen_US
dc.contributor.authorWong, TMen_US
dc.date.accessioned2012-05-29T06:03:53Z-
dc.date.available2012-05-29T06:03:53Z-
dc.date.issued2001en_US
dc.identifier.citationJournal Of Cardiovascular Pharmacology, 2001, v. 38 n. 2, p. 232-239en_US
dc.identifier.issn0160-2446en_US
dc.identifier.urihttp://hdl.handle.net/10722/147461-
dc.description.abstractTo explore the signaling mechanisms of the negative modulation of β-adrenoceptors by κ-opioid receptors (κ-OR) in the heart, the possibility of the interaction at the level of G protein and receptor was determined. Cholera toxin, an activator of the stimulatory G protein (Gs), elevated electrically induced intracellular Ca2+ ([Ca2+]i) transients and induced ribosylation of the α-subunit of Gs (Gsα) in rat ventricular myocytes. The effects were significantly attenuated by U50,488H, a specific agonist of κ-OR, and were abolished by nor-binaltorphimine, a selective κ-OR antagonist. The content of Gsα, however, was not affected by U50,488H. Receptor binding experiments showed that neither Bmax nor Kd of the binding of [3H]CGP-12177, a β-adrenoceptor antagonist, was affected by U50,488H. The current study provides the first evidence that κ-OR stimulation inhibits the ribosylation of the α-subunit of the Gs protein, thus inhibiting the action of cholera toxin on the protein.en_US
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.cardiovascularpharm.com/en_US
dc.relation.ispartofJournal of Cardiovascular Pharmacologyen_US
dc.subjectCholera toxin-sensitive G protein-
dc.subjectNorepinephrine-
dc.subjectPertussis toxin-sensitive G protein-
dc.subjectU50,488H-
dc.subjectVentricular myocyte-
dc.subject.mesh3,4-Dichloro-N-Methyl-N-(2-(1-Pyrrolidinyl)-Cyclohexyl)-Benzeneacetamide, (Trans)-Isomer - Pharmacologyen_US
dc.subject.meshAnalgesics, Non-Narcotic - Pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCalcium - Metabolismen_US
dc.subject.meshCholera Toxin - Pharmacologyen_US
dc.subject.meshElectric Stimulationen_US
dc.subject.meshGtp-Binding Protein Alpha Subunits, Gs - Antagonists & Inhibitors - Metabolismen_US
dc.subject.meshHeart Ventricles - Cytology - Drug Effectsen_US
dc.subject.meshMyocardium - Cytology - Metabolismen_US
dc.subject.meshPropanolamines - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshReceptors, Adrenergic, Beta - Metabolismen_US
dc.subject.meshReceptors, Opioid, Kappa - Agonists - Metabolism - Physiologyen_US
dc.titleκ-opioid receptor agonist inhibits the cholera toxin-sensitive G protein in the hearten_US
dc.typeArticleen_US
dc.identifier.emailWong, NS:nswong@hkucc.hku.hken_US
dc.identifier.authorityWong, NS=rp00340en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/00005344-200108000-00009en_US
dc.identifier.pmid11483873-
dc.identifier.scopuseid_2-s2.0-0034917623en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034917623&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume38en_US
dc.identifier.issue2en_US
dc.identifier.spage232en_US
dc.identifier.epage239en_US
dc.identifier.isiWOS:000170020200009-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridYu, XC=7404114600en_US
dc.identifier.scopusauthoridDiao, TM=7801649368en_US
dc.identifier.scopusauthoridPei, JM=7103299061en_US
dc.identifier.scopusauthoridZhang, WM=16235070800en_US
dc.identifier.scopusauthoridWong, NS=7202836641en_US
dc.identifier.scopusauthoridWong, TM=7403531434en_US
dc.identifier.issnl0160-2446-

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