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- Publisher Website: 10.1007/BF00351064
- Scopus: eid_2-s2.0-0026311236
- PMID: 1797232
- WOS: WOS:A1991GE80700006
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Article: The mouse Col2a-1 gene is highly conserved and is linked to Int-1 on Chromosome 15
Title | The mouse Col2a-1 gene is highly conserved and is linked to Int-1 on Chromosome 15 |
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Authors | |
Issue Date | 1991 |
Publisher | Springer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00335/ |
Citation | Mammalian Genome, 1991, v. 1 n. 3, p. 171-183 How to Cite? |
Abstract | Type II collagen is the major extracellular matrix component of cartilage and correct expression of the α1(II) collagen gene is important for vertebrate skeletal development. In order to provide the basis for studying the control of type II collagen gene expression in embryogenesis and in mouse models of human connective tissue disease, the complete mouse Col2-a1 gene has been isolated in a single cosmid clone, cosMcol.2, and partially characterized. The gene is approximately 30 kb and is highly conserved in exon/intron structure and nucleotide and amino acid sequence (>80% homology) when compared with the human, rat, bovine and chicken equivalents. A high degree of conservation was also found in the 5′ flanking region of the rat, human and mouse α1(II) collagen genes, including the presence of several G+C and C+T rich, direct repeat motifs. The sites of transcription start, termination codon and polyadenylation have also been identified. Unlike chicken, bovine and human, where polyA attachment is at a single site, for the mouse Col2a-1 gene two polyadenylation sites are utilized. Col2a-1 has also been localized by interspecies backcross analysis to the central portion of mouse Chromosome (Chr) 15, approximately 8 centiMorgans (cM) proximal of Int-1 and 18 cM distal of Myc. Col2a-1 is therefore included in a linkage group which is conserved on human Chr 12q. © 1991 Springer-Verlag. |
Persistent Identifier | http://hdl.handle.net/10722/147365 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.855 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Cheah, KSE | en_US |
dc.contributor.author | Au, PKC | en_US |
dc.contributor.author | Lau, ET | en_US |
dc.contributor.author | Little, PFR | en_US |
dc.contributor.author | Stubbs, L | en_US |
dc.date.accessioned | 2012-05-29T06:03:12Z | - |
dc.date.available | 2012-05-29T06:03:12Z | - |
dc.date.issued | 1991 | en_US |
dc.identifier.citation | Mammalian Genome, 1991, v. 1 n. 3, p. 171-183 | en_US |
dc.identifier.issn | 0938-8990 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/147365 | - |
dc.description.abstract | Type II collagen is the major extracellular matrix component of cartilage and correct expression of the α1(II) collagen gene is important for vertebrate skeletal development. In order to provide the basis for studying the control of type II collagen gene expression in embryogenesis and in mouse models of human connective tissue disease, the complete mouse Col2-a1 gene has been isolated in a single cosmid clone, cosMcol.2, and partially characterized. The gene is approximately 30 kb and is highly conserved in exon/intron structure and nucleotide and amino acid sequence (>80% homology) when compared with the human, rat, bovine and chicken equivalents. A high degree of conservation was also found in the 5′ flanking region of the rat, human and mouse α1(II) collagen genes, including the presence of several G+C and C+T rich, direct repeat motifs. The sites of transcription start, termination codon and polyadenylation have also been identified. Unlike chicken, bovine and human, where polyA attachment is at a single site, for the mouse Col2a-1 gene two polyadenylation sites are utilized. Col2a-1 has also been localized by interspecies backcross analysis to the central portion of mouse Chromosome (Chr) 15, approximately 8 centiMorgans (cM) proximal of Int-1 and 18 cM distal of Myc. Col2a-1 is therefore included in a linkage group which is conserved on human Chr 12q. © 1991 Springer-Verlag. | en_US |
dc.language | eng | en_US |
dc.publisher | Springer New York LLC. The Journal's web site is located at http://link.springer.de/link/service/journals/00335/ | en_US |
dc.relation.ispartof | Mammalian Genome | en_US |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Chromosome Mapping | en_US |
dc.subject.mesh | Cloning, Molecular | en_US |
dc.subject.mesh | Collagen - Genetics | en_US |
dc.subject.mesh | Crosses, Genetic | en_US |
dc.subject.mesh | Mice - Genetics | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Poly A - Metabolism | en_US |
dc.subject.mesh | Sequence Homology, Nucleic Acid | en_US |
dc.subject.mesh | Transcription, Genetic | en_US |
dc.subject.mesh | Vertebrates - Genetics | en_US |
dc.title | The mouse Col2a-1 gene is highly conserved and is linked to Int-1 on Chromosome 15 | en_US |
dc.type | Article | en_US |
dc.identifier.email | Cheah, KSE:hrmbdkc@hku.hk | en_US |
dc.identifier.authority | Cheah, KSE=rp00342 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/BF00351064 | en_US |
dc.identifier.pmid | 1797232 | en_US |
dc.identifier.scopus | eid_2-s2.0-0026311236 | en_US |
dc.identifier.volume | 1 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 171 | en_US |
dc.identifier.epage | 183 | en_US |
dc.identifier.isi | WOS:A1991GE80700006 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Cheah, KSE=35387746200 | en_US |
dc.identifier.scopusauthorid | Au, PKC=36789243400 | en_US |
dc.identifier.scopusauthorid | Lau, ET=7103086081 | en_US |
dc.identifier.scopusauthorid | Little, PFR=7201984979 | en_US |
dc.identifier.scopusauthorid | Stubbs, L=7006540193 | en_US |
dc.identifier.issnl | 0938-8990 | - |