File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Mouse ribosomal RNA genes contain multiple differentially regulated variants

TitleMouse ribosomal RNA genes contain multiple differentially regulated variants
Authors
Issue Date2008
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2008, v. 3 n. 3 How to Cite?
AbstractPrevious cytogenetic studies suggest that various rDNA chromosomal loci are not equally active in different cell types. Consistent with this variability, rDNA polymorphism is well documentaed in human and mouse. However, attempts to identify molecularly rDNA variant types, which are regulated individually (i.e independent of other rDNA variants) and tissue-specifically, have not been successful. We report here the molecuar cloning and characterization of seven mouse rDNA variants (v-rDNA). The identification of these v-rDNA was based on restriction fragment length polymorphisms (RFLPs) which are conserved among individuals and mouse strains. The total copy number of the identified variants is less than 100 and the copy number of each individual variant ranges from 4 to 15. Sequence analysis of the cloned v-rDNA identified variant-specific single nucleotide polymorphisms (SNPs) in the transcribed region. These SNPs were used to develop a set of variant-specific PCR assays, which permitted analysis of the v-rDNAs' expression profiles in various tissues. These profiles show that three v-rDNA are expressed in all tissues (constitutively active) two are expressed in some tissues (selectively active), and two are not expressed (silent). These expression profiles were observed in six individuals from three mouse variants, and some are regulated tissue-specifically. Our results provide the first molecular evidence for cell-type-specific regulation of a subset of rDNA. © 2008 Tseng et al.
Persistent Identifierhttp://hdl.handle.net/10722/145499
ISSN
2023 Impact Factor: 2.9
2023 SCImago Journal Rankings: 0.839
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
NIHAG14456
EY13637
HD22681
Funding Information:

This work was supported by NIH grants AG14456, EY13637 to HT, and HD22681 to RMS. The funding agencies had no role in the conception, design, conduct, analysis and reporting of this work.

References

 

DC FieldValueLanguage
dc.contributor.authorTseng, Hen_HK
dc.contributor.authorChou, Wen_HK
dc.contributor.authorWang, Jen_HK
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorZhang, Sen_HK
dc.contributor.authorSchultz, RMen_HK
dc.date.accessioned2012-02-24T07:09:03Z-
dc.date.available2012-02-24T07:09:03Z-
dc.date.issued2008en_HK
dc.identifier.citationPlos One, 2008, v. 3 n. 3en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/145499-
dc.description.abstractPrevious cytogenetic studies suggest that various rDNA chromosomal loci are not equally active in different cell types. Consistent with this variability, rDNA polymorphism is well documentaed in human and mouse. However, attempts to identify molecularly rDNA variant types, which are regulated individually (i.e independent of other rDNA variants) and tissue-specifically, have not been successful. We report here the molecuar cloning and characterization of seven mouse rDNA variants (v-rDNA). The identification of these v-rDNA was based on restriction fragment length polymorphisms (RFLPs) which are conserved among individuals and mouse strains. The total copy number of the identified variants is less than 100 and the copy number of each individual variant ranges from 4 to 15. Sequence analysis of the cloned v-rDNA identified variant-specific single nucleotide polymorphisms (SNPs) in the transcribed region. These SNPs were used to develop a set of variant-specific PCR assays, which permitted analysis of the v-rDNAs' expression profiles in various tissues. These profiles show that three v-rDNA are expressed in all tissues (constitutively active) two are expressed in some tissues (selectively active), and two are not expressed (silent). These expression profiles were observed in six individuals from three mouse variants, and some are regulated tissue-specifically. Our results provide the first molecular evidence for cell-type-specific regulation of a subset of rDNA. © 2008 Tseng et al.en_HK
dc.languageeng-
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subject.meshCloning, Molecular-
dc.subject.meshDNA, Ribosomal - genetics-
dc.subject.meshEpigenesis, Genetic-
dc.subject.meshPolymerase Chain Reaction-
dc.subject.meshRNA, Ribosomal - genetics-
dc.titleMouse ribosomal RNA genes contain multiple differentially regulated variantsen_HK
dc.typeArticleen_HK
dc.identifier.emailWang, J:junwen@hkucc.hku.hken_HK
dc.identifier.authorityWang, J=rp00280en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0001843en_HK
dc.identifier.pmid18365001-
dc.identifier.pmcidPMC2266999-
dc.identifier.scopuseid_2-s2.0-46649110132en_HK
dc.identifier.hkuros162379-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-46649110132&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume3en_HK
dc.identifier.issue3en_HK
dc.identifier.isiWOS:000260762400007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f10001103581-
dc.identifier.scopusauthoridTseng, H=19637691100en_HK
dc.identifier.scopusauthoridChou, W=24460592200en_HK
dc.identifier.scopusauthoridWang, J=8950599500en_HK
dc.identifier.scopusauthoridZhang, X=7410278895en_HK
dc.identifier.scopusauthoridZhang, S=7409376616en_HK
dc.identifier.scopusauthoridSchultz, RM=7401556658en_HK
dc.identifier.issnl1932-6203-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats