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- Publisher Website: 10.1016/j.jprot.2011.08.014
- Scopus: eid_2-s2.0-82355185714
- PMID: 21903181
- WOS: WOS:000298765500015
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Article: Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells
Title | Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells | ||||||||||||
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Authors | |||||||||||||
Keywords | Cytoskeleton Cytotoxicity ER Mitochondria Tubeimoside-1 | ||||||||||||
Issue Date | 2011 | ||||||||||||
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/713351/description#description | ||||||||||||
Citation | Journal Of Proteomics, 2011, v. 75 n. 2, p. 491-501 How to Cite? | ||||||||||||
Abstract | The Bolbostemma paniculatum (Maxim.) Franquet (Cucurbitaceae) is a Chinese herb with anticancer potential. Its main active component tubeimoside-1 (TBMS1), a triterpenoid saponin, was previously proved as a potent anticancer chemotherapeutic agent; however, the molecular basis for its activities is still elusive. In the present study, subcellular proteomic study in the cytoplasm and membrane protein fractions extracted from HeLa cells revealed that proteins act as mediators of ROS generation and Ca 2+ regulation were substantially altered in expression upon TBMS1 stimuli. We also found that TBMS1 induced cell cycle arrest at G2/M phase accompanied by a decrease in G0/G1 phase in HeLa cells. Further biochemical studies showed that TBMS1 inhibited the levels of cyclinB1, Cdc2 and Cdc25C, but enhanced Chk2 phosphorylation. In addition, the cytoplasm sequestration of Cdc25C, Cip1/p21 induction and tubulin dyspolymerization also contributed to the TBMS1-mediated cell cycle arrest on the G2/M phase. © 2011 Elsevier B.V. | ||||||||||||
Persistent Identifier | http://hdl.handle.net/10722/142314 | ||||||||||||
ISSN | 2023 Impact Factor: 2.8 2023 SCImago Journal Rankings: 0.742 | ||||||||||||
ISI Accession Number ID |
Funding Information: This work was partially supported by the 2007 Chang-Jiang Scholars Program, "211" Projects grant, National Natural Science Foundation of China (30973393), The Fundamental Research Funds for the Central Universities (to Q.-Y.H.); HKU Genomics, Proteomics and Bioinformatics Strategic Research Theme, Faculty of Science Development Fund, the Outstanding Young Researcher and Outstanding Research Student Supervisor Awards of the University of Hong Kong (to F.C.); and the Natural Science Foundation of Fujian Province of China (No. 2011 J05098) and the Fundamental Research Funds for the Central Universities (No. 2011121055) (to Y. X). | ||||||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xu, Y | en_HK |
dc.contributor.author | Ching, YP | en_HK |
dc.contributor.author | Zhou, Y | en_HK |
dc.contributor.author | Chiu, JF | en_HK |
dc.contributor.author | Chen, F | en_HK |
dc.contributor.author | He, QY | en_HK |
dc.date.accessioned | 2011-10-28T02:42:44Z | - |
dc.date.available | 2011-10-28T02:42:44Z | - |
dc.date.issued | 2011 | en_HK |
dc.identifier.citation | Journal Of Proteomics, 2011, v. 75 n. 2, p. 491-501 | en_HK |
dc.identifier.issn | 1874-3919 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/142314 | - |
dc.description.abstract | The Bolbostemma paniculatum (Maxim.) Franquet (Cucurbitaceae) is a Chinese herb with anticancer potential. Its main active component tubeimoside-1 (TBMS1), a triterpenoid saponin, was previously proved as a potent anticancer chemotherapeutic agent; however, the molecular basis for its activities is still elusive. In the present study, subcellular proteomic study in the cytoplasm and membrane protein fractions extracted from HeLa cells revealed that proteins act as mediators of ROS generation and Ca 2+ regulation were substantially altered in expression upon TBMS1 stimuli. We also found that TBMS1 induced cell cycle arrest at G2/M phase accompanied by a decrease in G0/G1 phase in HeLa cells. Further biochemical studies showed that TBMS1 inhibited the levels of cyclinB1, Cdc2 and Cdc25C, but enhanced Chk2 phosphorylation. In addition, the cytoplasm sequestration of Cdc25C, Cip1/p21 induction and tubulin dyspolymerization also contributed to the TBMS1-mediated cell cycle arrest on the G2/M phase. © 2011 Elsevier B.V. | en_HK |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/713351/description#description | en_HK |
dc.relation.ispartof | Journal of Proteomics | en_HK |
dc.subject | Cytoskeleton | en_HK |
dc.subject | Cytotoxicity | en_HK |
dc.subject | ER | en_HK |
dc.subject | Mitochondria | en_HK |
dc.subject | Tubeimoside-1 | en_HK |
dc.title | Multiple pathways were involved in tubeimoside-1-induced cytotoxicity of HeLa cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1874-3919&volume=&spage=&epage=&date=2011&atitle=Multiple+pathways+were+involved+in+tubeimoside-1-induced+cytotoxicity+of+HeLa+cells | en_US |
dc.identifier.email | Ching, YP: ypching@hku.hk | en_HK |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_HK |
dc.identifier.authority | Ching, YP=rp00469 | en_HK |
dc.identifier.authority | Chen, F=rp00672 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jprot.2011.08.014 | en_HK |
dc.identifier.pmid | 21903181 | - |
dc.identifier.scopus | eid_2-s2.0-82355185714 | en_HK |
dc.identifier.hkuros | 196627 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-82355185714&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 75 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 491 | en_HK |
dc.identifier.epage | 501 | en_HK |
dc.identifier.isi | WOS:000298765500015 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Xu, Y=37035700000 | en_HK |
dc.identifier.scopusauthorid | Ching, YP=7005431277 | en_HK |
dc.identifier.scopusauthorid | Zhou, Y=54786121900 | en_HK |
dc.identifier.scopusauthorid | Chiu, JF=7201501692 | en_HK |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_HK |
dc.identifier.scopusauthorid | He, QY=34770287900 | en_HK |
dc.identifier.issnl | 1874-3919 | - |