File Download
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1128/JVI.02364-10
- Scopus: eid_2-s2.0-80052275129
- PMID: 21697464
- WOS: WOS:000293626100034
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Complete genome analysis of three novel picornaviruses from diverse bat species
Title | Complete genome analysis of three novel picornaviruses from diverse bat species | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Authors | |||||||||||||||||||||||||
Issue Date | 2011 | ||||||||||||||||||||||||
Publisher | American Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/ | ||||||||||||||||||||||||
Citation | Journal Of Virology, 2011, v. 85 n. 17, p. 8819-8828 How to Cite? | ||||||||||||||||||||||||
Abstract | Although bats are important reservoirs of diverse viruses that can cause human epidemics, little is known about the presence of picornaviruses in these flying mammals. Among 1,108 bats of 18 species studied, three novel picornaviruses (groups 1, 2, and 3) were identified from alimentary specimens of 12 bats from five species and four genera. Two complete genomes, each from the three picornaviruses, were sequenced. Phylogenetic analysis showed that they fell into three distinct clusters in the Picornaviridae family, with low homologies to known picornaviruses, especially in leader and 2A proteins. Moreover, group 1 and 2 viruses are more closely related to each other than to group 3 viruses, which exhibit genome features distinct from those of the former two virus groups. In particular, the group 3 virus genome contains the shortest leader protein within Picornaviridae, a putative type I internal ribosome entry site (IRES) in the 5′-untranslated region instead of the type IV IRES found in group 1 and 2 viruses, one instead of two GXCG motifs in 2A, an L→V substitution in the DDLXQ motif in 2C helicase, and a conserved GXH motif in 3C protease. Group 1 and 2 viruses are unique among picornaviruses in having AMH instead of the GXH motif in 3C pro. These findings suggest that the three picornaviruses belong to two novel genera in the Picornaviridae family. This report describes the discovery and complete genome analysis of three picornaviruses in bats, and their presence in diverse bat genera/species suggests the ability to cross the species barrier. © 2011, American Society for Microbiology. | ||||||||||||||||||||||||
Persistent Identifier | http://hdl.handle.net/10722/139512 | ||||||||||||||||||||||||
ISSN | 2023 Impact Factor: 4.0 2023 SCImago Journal Rankings: 1.378 | ||||||||||||||||||||||||
PubMed Central ID | |||||||||||||||||||||||||
ISI Accession Number ID |
Funding Information: We thank Alan Chi-Kong Wong, Siu-Fai Leung, Chik-Chuen Lay, Ping-Man So, and K. F. Chan (HKSAR Department of Agriculture, Fisheries, and Conservation [AFCD]) and the Hong Kong Police Force for facilitation and support; Cynthia S. M. Chan and Joseph W. K. So from AFCD; and King-Shun Lo (Laboratory Animal Unit) and Cassius Chan for their excellent technical assistance and collection of animal specimens. We are grateful for the generous support of Carol Yu, Richard Yu, Hui Hoy, and Hui Ming in the genomic sequencing platform. | ||||||||||||||||||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lau, SKP | en_HK |
dc.contributor.author | Woo, PCY | en_HK |
dc.contributor.author | Lai, KKY | en_HK |
dc.contributor.author | Huang, Y | en_HK |
dc.contributor.author | Yip, CCY | en_HK |
dc.contributor.author | Shek, CT | en_HK |
dc.contributor.author | Lee, P | en_HK |
dc.contributor.author | Lam, CSF | en_HK |
dc.contributor.author | Chan, KH | en_HK |
dc.contributor.author | Yuen, KY | en_HK |
dc.date.accessioned | 2011-09-23T05:50:57Z | - |
dc.date.available | 2011-09-23T05:50:57Z | - |
dc.date.issued | 2011 | en_HK |
dc.identifier.citation | Journal Of Virology, 2011, v. 85 n. 17, p. 8819-8828 | en_HK |
dc.identifier.issn | 0022-538X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/139512 | - |
dc.description.abstract | Although bats are important reservoirs of diverse viruses that can cause human epidemics, little is known about the presence of picornaviruses in these flying mammals. Among 1,108 bats of 18 species studied, three novel picornaviruses (groups 1, 2, and 3) were identified from alimentary specimens of 12 bats from five species and four genera. Two complete genomes, each from the three picornaviruses, were sequenced. Phylogenetic analysis showed that they fell into three distinct clusters in the Picornaviridae family, with low homologies to known picornaviruses, especially in leader and 2A proteins. Moreover, group 1 and 2 viruses are more closely related to each other than to group 3 viruses, which exhibit genome features distinct from those of the former two virus groups. In particular, the group 3 virus genome contains the shortest leader protein within Picornaviridae, a putative type I internal ribosome entry site (IRES) in the 5′-untranslated region instead of the type IV IRES found in group 1 and 2 viruses, one instead of two GXCG motifs in 2A, an L→V substitution in the DDLXQ motif in 2C helicase, and a conserved GXH motif in 3C protease. Group 1 and 2 viruses are unique among picornaviruses in having AMH instead of the GXH motif in 3C pro. These findings suggest that the three picornaviruses belong to two novel genera in the Picornaviridae family. This report describes the discovery and complete genome analysis of three picornaviruses in bats, and their presence in diverse bat genera/species suggests the ability to cross the species barrier. © 2011, American Society for Microbiology. | en_HK |
dc.language | eng | en_US |
dc.publisher | American Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/ | en_HK |
dc.relation.ispartof | Journal of Virology | en_HK |
dc.rights | Journal of Virology. Copyright © American Society for Microbiology. | - |
dc.subject.mesh | Chiroptera - virology | - |
dc.subject.mesh | Genome, Viral | - |
dc.subject.mesh | Picornaviridae - classification - genetics - isolation and purification | - |
dc.subject.mesh | RNA, Viral - genetics | - |
dc.subject.mesh | Sequence Analysis, DNA | - |
dc.title | Complete genome analysis of three novel picornaviruses from diverse bat species | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=85&issue=17&spage=8819&epage=8828&date=2011&atitle=Complete+genome+analysis+of+three+novel+picornaviruses+from+diverse+bat+species | - |
dc.identifier.email | Lau, SKP: skplau@hkucc.hku.hk | en_HK |
dc.identifier.email | Woo, PCY: pcywoo@hkucc.hku.hk | en_HK |
dc.identifier.email | Yip, CCY: yipcyril@hku.hk | en_HK |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | en_HK |
dc.identifier.authority | Lau, SKP=rp00486 | en_HK |
dc.identifier.authority | Woo, PCY=rp00430 | en_HK |
dc.identifier.authority | Yip, CCY=rp01721 | en_HK |
dc.identifier.authority | Yuen, KY=rp00366 | en_HK |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1128/JVI.02364-10 | en_HK |
dc.identifier.pmid | 21697464 | - |
dc.identifier.pmcid | PMC3165794 | - |
dc.identifier.scopus | eid_2-s2.0-80052275129 | en_HK |
dc.identifier.hkuros | 192207 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-80052275129&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 85 | en_HK |
dc.identifier.issue | 17 | en_HK |
dc.identifier.spage | 8819 | en_HK |
dc.identifier.epage | 8828 | en_HK |
dc.identifier.isi | WOS:000293626100034 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Lau, SKP=7401596211 | en_HK |
dc.identifier.scopusauthorid | Woo, PCY=7201801340 | en_HK |
dc.identifier.scopusauthorid | Lai, KKY=36708103200 | en_HK |
dc.identifier.scopusauthorid | Huang, Y=35597414700 | en_HK |
dc.identifier.scopusauthorid | Yip, CCY=14016999800 | en_HK |
dc.identifier.scopusauthorid | Shek, CT=36007758300 | en_HK |
dc.identifier.scopusauthorid | Lee, P=36189338700 | en_HK |
dc.identifier.scopusauthorid | Lam, CSF=25950267400 | en_HK |
dc.identifier.scopusauthorid | Chan, KH=7406034307 | en_HK |
dc.identifier.scopusauthorid | Yuen, KY=36078079100 | en_HK |
dc.identifier.issnl | 0022-538X | - |