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Article: Factors affecting mesenchymal stromal cells yield from bone marrow aspiration

TitleFactors affecting mesenchymal stromal cells yield from bone marrow aspiration
Authors
KeywordsBone marrow aspiration
CFU-F
Leukemia
Mesenchymal stromal cell
Issue Date2011
PublisherChina Anti-Cancer Association. The Journal's web site is located at http://zgazyj.periodicals.net.cn/
Citation
Chinese Journal Of Cancer Research, 2011, v. 23 n. 1, p. 43-48 How to Cite?
AbstractObjective: This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC). Methods: We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles. Results: During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors <55 years. And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle. Conclusion: The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion. © 2011 Chinese Anti-Cancer Association and Springer.
Persistent Identifierhttp://hdl.handle.net/10722/137476
ISSN
2023 Impact Factor: 7.0
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Jen_HK
dc.contributor.authorWong, WHSen_HK
dc.contributor.authorChan, Sen_HK
dc.contributor.authorChim, JCSen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorLee, TLen_HK
dc.contributor.authorAu, WYen_HK
dc.contributor.authorHa, SYen_HK
dc.contributor.authorLie, AKWen_HK
dc.contributor.authorLau, YLen_HK
dc.contributor.authorLiang, RHSen_HK
dc.contributor.authorChan, GCFen_HK
dc.date.accessioned2011-08-26T14:25:50Z-
dc.date.available2011-08-26T14:25:50Z-
dc.date.issued2011en_HK
dc.identifier.citationChinese Journal Of Cancer Research, 2011, v. 23 n. 1, p. 43-48en_HK
dc.identifier.issn1000-9604en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137476-
dc.description.abstractObjective: This study was to investigate the variables in bone marrow harvesting procedure and individual donor factors which can potentially affect the yield of mesenchymal stromal cells (MSC). Methods: We determined the yield of MSC from bone marrow under different clinical conditions by comparing the MSC colony numbers from: (1) donors of different ages; (2) healthy donors and patients with leukemia; (3) bone marrow aspirated at different time points during marrow harvesting; (4) bone marrow harvested by different needles. Results: During the process of harvesting, the number of MSC significantly decreased with increase number of aspiration, from 675/ml at the initial decreased to 60/ml after 100 ml bone marrow aspirated, and 50/ml after 200 ml bone marrow aspirated. The number of MSC retrieved from leukemia patients (99/ml bone marrow) was significantly lower than that of healthy donors (708/ml bone marrow). However, there was no significant difference in growth rate. There was no significant age-related difference of MSC yielded from donors <55 years. And there was no significant difference in MSC number between the samples from single end-holed needle and those from multiple-side-hole needle. Conclusion: The optimal bone marrow samples for MSC collection should be obtained earlier in the process of harvesting procedure. Bone marrow from donors <55 years was equally good as MSC sources. The autologous MSC from leukemia patients can be utilized for in-vitro MSC expansion. © 2011 Chinese Anti-Cancer Association and Springer.en_HK
dc.languageengen_US
dc.publisherChina Anti-Cancer Association. The Journal's web site is located at http://zgazyj.periodicals.net.cn/en_HK
dc.relation.ispartofChinese Journal of Cancer Researchen_HK
dc.subjectBone marrow aspirationen_HK
dc.subjectCFU-Fen_HK
dc.subjectLeukemiaen_HK
dc.subjectMesenchymal stromal cellen_HK
dc.titleFactors affecting mesenchymal stromal cells yield from bone marrow aspirationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1000-9604&volume=23&issue=1&spage=43&epage=48&date=2011&atitle=Factors+affecting+mesenchymal+stromal+cells+yield+from+bone+marrow+aspirationen_US
dc.identifier.emailChim, JCS:jcschim@hku.hken_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.emailLiang, RHS:rliang@hku.hken_HK
dc.identifier.emailChan, GCF:gcfchan@hkucc.hku.hken_HK
dc.identifier.authorityChim, JCS=rp00408en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.identifier.authorityLiang, RHS=rp00345en_HK
dc.identifier.authorityChan, GCF=rp00431en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11670-011-0043-1en_HK
dc.identifier.scopuseid_2-s2.0-79953210080en_HK
dc.identifier.hkuros191919en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79953210080&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue1en_HK
dc.identifier.spage43en_HK
dc.identifier.epage48en_HK
dc.identifier.isiWOS:000290804200009-
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridLi, J=36078695800en_HK
dc.identifier.scopusauthoridWong, WHS=13310222200en_HK
dc.identifier.scopusauthoridChan, S=35071039700en_HK
dc.identifier.scopusauthoridChim, JCS=7004597253en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridLee, TL=35573927500en_HK
dc.identifier.scopusauthoridAu, WY=7202383089en_HK
dc.identifier.scopusauthoridHa, SY=7202501115en_HK
dc.identifier.scopusauthoridLie, AKW=7004510870en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.scopusauthoridLiang, RHS=26643224900en_HK
dc.identifier.scopusauthoridChan, GCF=16160154400en_HK
dc.identifier.citeulike9004087-
dc.identifier.issnl1000-9604-

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