File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1021/es103672x
- Scopus: eid_2-s2.0-79953240174
- PMID: 21388174
- WOS: WOS:000288841500017
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Occurrence, abundance, and diversity of tetracycline resistance genes in 15 sewage treatment plants across china and other global locations
Title | Occurrence, abundance, and diversity of tetracycline resistance genes in 15 sewage treatment plants across china and other global locations | ||||||||
---|---|---|---|---|---|---|---|---|---|
Authors | |||||||||
Issue Date | 2011 | ||||||||
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/est | ||||||||
Citation | Environmental Science And Technology, 2011, v. 45 n. 7, p. 2598-2604 How to Cite? | ||||||||
Abstract | Activated sludge was sampled from 15 sewage treatment plants (STPs) across China and other global locations to investigate the occurrence, abundance and diversity of tetracycline resistance genes (tet) in the STPs. Occurrence and abundance of 14 tet genes were determined using polymerase chain reaction (PCR) and quantitative real time PCR. Six genes (tet(A), tet(C), tet(G), tet(M), tet(S), and tet(X)) were detected in all the STPs, while no sludge sample contained tet(Q). Total concentration of the 14 genes was significantly different among the STPs and average tet abundance of the STPs varied greatly among the tet types (p < 0.05). Tet(G) had the highest concentration in the STPs, followed by tet(C), tet(A) and tet(S). Phylogenetic diversity of the genes was investigated using DNA cloning. BLAST analysis showed that all of the 450 cloned sequences matched known tet genes, except for tet(G). The 56 tet(G) clones were grouped into 14 genotypes, among which type G24 had an identical sequence to tet(G) carried by Salmonella enterica or Acinetobacter baumannii, while the other sequences had low similarity to the known genes in GenBank. The results of this study might be useful to understand the diversity of these resistance genes in STPs. © 2011 American Chemical Society. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/137227 | ||||||||
ISSN | 2023 Impact Factor: 10.8 2023 SCImago Journal Rankings: 3.516 | ||||||||
ISI Accession Number ID |
Funding Information: This study was financially supported by the Research Grants Council of Hong Kong (HKU7202/09E), Small Project Fund (201007176069), and SPACE Research Fund of the University of Hong Kong. We would like to thank Prof. Gao DW, Prof. Deng BL, Dr. Huang QC, Dr. Zhu HG, Dr. Liang DW, Dr. Duan JZ, Dr. Zhang M, Mr. Yu K, Miss Yang Y, and Mr. Ye L for the sludge sampling. | ||||||||
References | |||||||||
Grants |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, XX | en_HK |
dc.contributor.author | Zhang, T | en_HK |
dc.date.accessioned | 2011-08-26T14:21:35Z | - |
dc.date.available | 2011-08-26T14:21:35Z | - |
dc.date.issued | 2011 | en_HK |
dc.identifier.citation | Environmental Science And Technology, 2011, v. 45 n. 7, p. 2598-2604 | en_HK |
dc.identifier.issn | 0013-936X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/137227 | - |
dc.description.abstract | Activated sludge was sampled from 15 sewage treatment plants (STPs) across China and other global locations to investigate the occurrence, abundance and diversity of tetracycline resistance genes (tet) in the STPs. Occurrence and abundance of 14 tet genes were determined using polymerase chain reaction (PCR) and quantitative real time PCR. Six genes (tet(A), tet(C), tet(G), tet(M), tet(S), and tet(X)) were detected in all the STPs, while no sludge sample contained tet(Q). Total concentration of the 14 genes was significantly different among the STPs and average tet abundance of the STPs varied greatly among the tet types (p < 0.05). Tet(G) had the highest concentration in the STPs, followed by tet(C), tet(A) and tet(S). Phylogenetic diversity of the genes was investigated using DNA cloning. BLAST analysis showed that all of the 450 cloned sequences matched known tet genes, except for tet(G). The 56 tet(G) clones were grouped into 14 genotypes, among which type G24 had an identical sequence to tet(G) carried by Salmonella enterica or Acinetobacter baumannii, while the other sequences had low similarity to the known genes in GenBank. The results of this study might be useful to understand the diversity of these resistance genes in STPs. © 2011 American Chemical Society. | en_HK |
dc.language | eng | en_US |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/est | en_HK |
dc.relation.ispartof | Environmental Science and Technology | en_HK |
dc.subject.mesh | Bacteria - classification - genetics - isolation and purification | - |
dc.subject.mesh | Environmental Monitoring | - |
dc.subject.mesh | Sewage - microbiology | - |
dc.subject.mesh | Tetracycline Resistance - genetics | - |
dc.subject.mesh | Waste Disposal, Fluid | - |
dc.title | Occurrence, abundance, and diversity of tetracycline resistance genes in 15 sewage treatment plants across china and other global locations | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Zhang, T:zhangt@hkucc.hku.hk | en_HK |
dc.identifier.authority | Zhang, T=rp00211 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1021/es103672x | en_HK |
dc.identifier.pmid | 21388174 | - |
dc.identifier.scopus | eid_2-s2.0-79953240174 | en_HK |
dc.identifier.hkuros | 190128 | en_US |
dc.identifier.hkuros | 208074 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79953240174&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 45 | en_HK |
dc.identifier.issue | 7 | en_HK |
dc.identifier.spage | 2598 | en_HK |
dc.identifier.epage | 2604 | en_HK |
dc.identifier.isi | WOS:000288841500017 | - |
dc.publisher.place | United States | en_HK |
dc.relation.project | Occurrence, abundance and diversity of tetracycline resistance genes in sewage treatment plants | - |
dc.identifier.scopusauthorid | Zhang, XX=35239161000 | en_HK |
dc.identifier.scopusauthorid | Zhang, T=24470677400 | en_HK |
dc.identifier.issnl | 0013-936X | - |