File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.jcf.2004.05.046
- Scopus: eid_2-s2.0-19944414292
- PMID: 15463939
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel
Title | The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel |
---|---|
Authors | |
Keywords | Anion permeation Channel gating Channel regulation Chloride channel activity Single-channel recording Whole-cell recording |
Issue Date | 2004 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcf |
Citation | Journal Of Cystic Fibrosis, 2004, v. 3 SUPPL. 2, p. 101-108 How to Cite? |
Abstract | Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties. © 2004 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/137018 |
ISSN | 2023 Impact Factor: 5.4 2023 SCImago Journal Rankings: 2.043 |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sheppard, DN | en_HK |
dc.contributor.author | Gray, MA | en_HK |
dc.contributor.author | Gong, X | en_HK |
dc.contributor.author | Sohma, Y | en_HK |
dc.contributor.author | Kogan, I | en_HK |
dc.contributor.author | Benos, DJ | en_HK |
dc.contributor.author | ScottWard, TS | en_HK |
dc.contributor.author | Chen, JH | en_HK |
dc.contributor.author | Li, H | en_HK |
dc.contributor.author | Cai, Z | en_HK |
dc.contributor.author | Gupta, J | en_HK |
dc.contributor.author | Li, C | en_HK |
dc.contributor.author | Ramjeesingh, M | en_HK |
dc.contributor.author | Berdiev, BK | en_HK |
dc.contributor.author | Ismailov, II | en_HK |
dc.contributor.author | Bear, CE | en_HK |
dc.contributor.author | Hwang, TC | en_HK |
dc.contributor.author | Linsdell, P | en_HK |
dc.contributor.author | Hug, MJ | en_HK |
dc.date.accessioned | 2011-07-29T02:14:22Z | - |
dc.date.available | 2011-07-29T02:14:22Z | - |
dc.date.issued | 2004 | en_HK |
dc.identifier.citation | Journal Of Cystic Fibrosis, 2004, v. 3 SUPPL. 2, p. 101-108 | en_HK |
dc.identifier.issn | 1569-1993 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/137018 | - |
dc.description.abstract | Using the patch-clamp (PC) and planar lipid bilayer (PLB) techniques the molecular behaviour of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel can be visualised in real-time. The PC technique is a highly powerful and versatile method to investigate CFTR's mechanism of action, interaction with other proteins and physiological role. Using the PLB technique, the structure and function of CFTR can be investigated free from the influence of other proteins. Here we discuss how these techniques are employed to investigate the CFTR Cl- channel with special emphasis on its permeation, conduction and gating properties. © 2004 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved. | en_HK |
dc.language | eng | en_US |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcf | en_HK |
dc.relation.ispartof | Journal of Cystic Fibrosis | en_HK |
dc.subject | Anion permeation | en_HK |
dc.subject | Channel gating | en_HK |
dc.subject | Channel regulation | en_HK |
dc.subject | Chloride channel activity | en_HK |
dc.subject | Single-channel recording | en_HK |
dc.subject | Whole-cell recording | en_HK |
dc.title | The patch-clamp and planar lipid bilayer techniques: Powerful and versatile tools to investigate the CFTR Cl- channel | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Chen, JH: jhlchen@hku.hk | en_HK |
dc.identifier.authority | Chen, JH=rp01518 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.jcf.2004.05.046 | en_HK |
dc.identifier.pmid | 15463939 | - |
dc.identifier.scopus | eid_2-s2.0-19944414292 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-19944414292&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 3 | en_HK |
dc.identifier.issue | SUPPL. 2 | en_HK |
dc.identifier.spage | 101 | en_HK |
dc.identifier.epage | 108 | en_HK |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Sheppard, DN=7201812458 | en_HK |
dc.identifier.scopusauthorid | Gray, MA=7401585136 | en_HK |
dc.identifier.scopusauthorid | Gong, X=7201999007 | en_HK |
dc.identifier.scopusauthorid | Sohma, Y=7004865261 | en_HK |
dc.identifier.scopusauthorid | Kogan, I=7102422686 | en_HK |
dc.identifier.scopusauthorid | Benos, DJ=7101816769 | en_HK |
dc.identifier.scopusauthorid | ScottWard, TS=6506239769 | en_HK |
dc.identifier.scopusauthorid | Chen, JH=7501878156 | en_HK |
dc.identifier.scopusauthorid | Li, H=24468545300 | en_HK |
dc.identifier.scopusauthorid | Cai, Z=7402905250 | en_HK |
dc.identifier.scopusauthorid | Gupta, J=36762073000 | en_HK |
dc.identifier.scopusauthorid | Li, C=7501673777 | en_HK |
dc.identifier.scopusauthorid | Ramjeesingh, M=7003391689 | en_HK |
dc.identifier.scopusauthorid | Berdiev, BK=7004021700 | en_HK |
dc.identifier.scopusauthorid | Ismailov, II=35570991100 | en_HK |
dc.identifier.scopusauthorid | Bear, CE=7006718679 | en_HK |
dc.identifier.scopusauthorid | Hwang, TC=7202849062 | en_HK |
dc.identifier.scopusauthorid | Linsdell, P=7004694661 | en_HK |
dc.identifier.scopusauthorid | Hug, MJ=7005762371 | en_HK |
dc.identifier.issnl | 1569-1993 | - |