File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The decrement in circulating endothelial progenitor cells (EPCs) in type 2 diabetes is independent of the severity of the hypoadiponectemia

TitleThe decrement in circulating endothelial progenitor cells (EPCs) in type 2 diabetes is independent of the severity of the hypoadiponectemia
Authors
KeywordsAdiponectin
Endothelial progenitor cells
Type 2 diabetes mellitus
Issue Date2011
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10009394
Citation
Diabetes/Metabolism Research And Reviews, 2011, v. 27 n. 2, p. 185-194 How to Cite?
AbstractBackground: Type 2 diabetes mellitus (DM) is associated with a decreased level of circulating endothelial progenitor cells (EPCs) and adiponectin. Experimental studies suggest a potential link between hypoadiponectinaemia and the depletion of the EPC level. This study investigated the relationships between adiponectin level and EPC in patients with type 2 DM. Methods: A total of 95 type 2 DM patients (58.5 ± 8.8 years, 42 men) and 95 age- and sex-matched healthy controls were recruited. Circulating EPC levels were determined by flow cytometry using CD133 +, CD34 +, CD133 +/KDR + and CD34 +/KDR + as surface markers. Plasma adiponectin levels were measured by enzyme-linked immunosorbent assay. EPC function was studied by in vitro tube formation and migration assay. Results: The levels of CD133 + (p < 0.001) and CD133 +/KDR + (p < 0.001) EPCs were independently associated with the presence of type 2 DM. The levels of CD34 + (p = 0.004) and CD34 +/KDR + (p = 0.013) EPCs were independently associated with haemoglobin A 1c. Nevertheless, there was no relationship between the number of EPCs and adiponectin level. Tube formation assay showed impaired pro-angiogenic function of EPC in DM patients compared with controls (p = 0.007). Interestingly, adiponectin supplementation (5 μg/mL) increased tube formation by 17.6% in EPCs from DM patients (p = 0.002). It also significantly enhanced cell migration by 35.9% in EPCs from DM patients (p = 0.01). Conclusions: We detected no relationship between the reduction in the level of EPC and in the level of total adiponectin in blood from patients with type 2 diabetes. EPC from patients with diabetes were stimulated when exposed to adiponectin in the test tube, findings that warrant further study. Copyright © 2011 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/135213
ISSN
2023 Impact Factor: 4.6
2023 SCImago Journal Rankings: 1.991
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilHKU 8/CRF/09
University of Hong Kong200907176063
Sun Chieh Yeh Heart Foundation
Funding Information:

This study was supported by Collaborative Research Fund of Hong Kong Research Grant Council (HKU 8/CRF/09), Outstanding Researcher Award of the University of Hong Kong (HF Tse), CRCG Small Project Funding of University of Hong Kong (Project No. 200907176063), and Sun Chieh Yeh Heart Foundation.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLi, Men_HK
dc.contributor.authorHo, JCYen_HK
dc.contributor.authorLai, KWHen_HK
dc.contributor.authorAu, KKWen_HK
dc.contributor.authorXu, Aen_HK
dc.contributor.authorCheung, BMYen_HK
dc.contributor.authorLam, KSLen_HK
dc.contributor.authorTse, HFen_HK
dc.date.accessioned2011-07-27T01:30:03Z-
dc.date.available2011-07-27T01:30:03Z-
dc.date.issued2011en_HK
dc.identifier.citationDiabetes/Metabolism Research And Reviews, 2011, v. 27 n. 2, p. 185-194en_HK
dc.identifier.issn1520-7552en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135213-
dc.description.abstractBackground: Type 2 diabetes mellitus (DM) is associated with a decreased level of circulating endothelial progenitor cells (EPCs) and adiponectin. Experimental studies suggest a potential link between hypoadiponectinaemia and the depletion of the EPC level. This study investigated the relationships between adiponectin level and EPC in patients with type 2 DM. Methods: A total of 95 type 2 DM patients (58.5 ± 8.8 years, 42 men) and 95 age- and sex-matched healthy controls were recruited. Circulating EPC levels were determined by flow cytometry using CD133 +, CD34 +, CD133 +/KDR + and CD34 +/KDR + as surface markers. Plasma adiponectin levels were measured by enzyme-linked immunosorbent assay. EPC function was studied by in vitro tube formation and migration assay. Results: The levels of CD133 + (p < 0.001) and CD133 +/KDR + (p < 0.001) EPCs were independently associated with the presence of type 2 DM. The levels of CD34 + (p = 0.004) and CD34 +/KDR + (p = 0.013) EPCs were independently associated with haemoglobin A 1c. Nevertheless, there was no relationship between the number of EPCs and adiponectin level. Tube formation assay showed impaired pro-angiogenic function of EPC in DM patients compared with controls (p = 0.007). Interestingly, adiponectin supplementation (5 μg/mL) increased tube formation by 17.6% in EPCs from DM patients (p = 0.002). It also significantly enhanced cell migration by 35.9% in EPCs from DM patients (p = 0.01). Conclusions: We detected no relationship between the reduction in the level of EPC and in the level of total adiponectin in blood from patients with type 2 diabetes. EPC from patients with diabetes were stimulated when exposed to adiponectin in the test tube, findings that warrant further study. Copyright © 2011 John Wiley & Sons, Ltd.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10009394en_HK
dc.relation.ispartofDiabetes/Metabolism Research and Reviewsen_HK
dc.rightsDiabetes - Metabolism: Research and Reviews. Copyright © John Wiley & Sons Ltd.-
dc.subjectAdiponectinen_HK
dc.subjectEndothelial progenitor cellsen_HK
dc.subjectType 2 diabetes mellitusen_HK
dc.subject.meshAdiponectin - blood-
dc.subject.meshCells, Cultured-
dc.subject.meshDiabetes Mellitus, Type 2 - blood - pathology-
dc.subject.meshEndothelium, Vascular - cytology - metabolism-
dc.subject.meshStem Cells - metabolism-
dc.titleThe decrement in circulating endothelial progenitor cells (EPCs) in type 2 diabetes is independent of the severity of the hypoadiponectemiaen_HK
dc.typeArticleen_HK
dc.identifier.emailXu, A:amxu@hkucc.hku.hken_HK
dc.identifier.emailCheung, BMY:mycheung@hku.hken_HK
dc.identifier.emailLam, KSL:ksllam@hku.hken_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.identifier.authorityCheung, BMY=rp01321en_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/dmrr.1159en_HK
dc.identifier.pmid21294240-
dc.identifier.scopuseid_2-s2.0-79551584062en_HK
dc.identifier.hkuros187099en_US
dc.identifier.hkuros174101-
dc.identifier.hkuros183463-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79551584062&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume27en_HK
dc.identifier.issue2en_HK
dc.identifier.spage185en_HK
dc.identifier.epage194en_HK
dc.identifier.isiWOS:000287155500005-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectPluripotent Human Stem Cell Platform for Tissue Regeneration and Drug Screening for Cardiovascular Diseases-
dc.identifier.scopusauthoridLi, M=36465410000en_HK
dc.identifier.scopusauthoridHo, JCY=7402650173en_HK
dc.identifier.scopusauthoridLai, KWH=7402135541en_HK
dc.identifier.scopusauthoridAu, KKW=38662193900en_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK
dc.identifier.scopusauthoridCheung, BMY=7103294806en_HK
dc.identifier.scopusauthoridLam, KSL=8082870600en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.issnl1520-7552-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats