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Article: SOX9 induces and maintains neural stem cells

TitleSOX9 induces and maintains neural stem cells
Authors
Scott, CEWynn, SLSesay, ACruz, CCheung, MGaviro, MVGBooth, SGao, BCheah, KSELovellBadge, RBriscoe, J
Issue Date2010
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/neuro/
Citation
Nature Neuroscience, 2010, v. 13 n. 10, p. 1181-1189 How to Cite?
DOI: http://dx.doi.org/10.1038/nn.2646
AbstractNeural stem cells (NSCs) are uncommitted cells of the CNS defined by their multipotentiality and ability to self renew. We found these cells to not be present in substantial numbers in the CNS until after embryonic day (E) 10.5 in mouse and E5 in chick. This coincides with the induction of SOX9 in neural cells. Gain-and loss-of-function studies indicated that SOX9 was essential for multipotent NSC formation. Moreover, Sonic Hedgehog was able to stimulate precocious generation of NSCs by inducing Sox9 expression. SOX9 was also necessary for the maintenance of multipotent NSCs, as shown by in vivo fate mapping experiments in the adult subependymal zone and olfactory bulbs. In addition, loss of SOX9 led ependymal cells to adopt a neuroblast identity. These data identify a functional link between extrinsic and intrinsic mechanisms of NSCs specification and maintenance, and establish a central role for SOX9 in the process. © 2010 Nature America, Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/133582
ISSN
1097-6256
2023 Impact Factor: 21.2
2023 SCImago Journal Rankings: 12.261
ISI Accession Number ID
WOS:000282171300009
Funding AgencyGrant Number
UK Medical Research Council
US National Institutes of Health (National Institute of Biomedical Imaging and Bioengineering)U117512772
Hong Kong Research Grants Council
Funding Information:

We thank A. Schedl (INSERM U636, Nice) for the conditional Sox9 mutant mice, A. Nagy (Samuel Lunenfeld Research Institute) for the pCall2 vector, W.C.W. Chan for blastocyst injections and production of Z/Sox9 mice and T. Caspary (Emory University) for the Arl13b antibody. The CMV:cre construct was a gift from S.O'Gorman (Salk Institute). Thank you to S. Guioli, F. Guillemot and L. Reynard for critical reading of the manuscript, to C. Andoniadou for advice and training in the ways of neurosphere cultures, to W. Han Yau in the photographics department at NIMR for help with illustrations, to T. Matabanadzo and other biological services staff at NIMR for help with the mouse colonies and other members of our laboratories for discussion and encouragement. This work was supported by the UK Medical Research Council (U117512772), a US National Institutes of Health (National Institute of Biomedical Imaging and Bioengineering) Quantum Grant (R.L.-B. and C.E.S.), and grants from the Hong Kong Research Grants Council and the Hong Kong University Grants Council Area of Excellence Scheme (S.L.W. and K.S.E.C.).

References
References in Scopus
Grants
Developmental genomics and skeletal research

 

DC FieldValueLanguage
dc.contributor.authorScott, CEen_HK
dc.contributor.authorWynn, SLen_HK
dc.contributor.authorSesay, Aen_HK
dc.contributor.authorCruz, Cen_HK
dc.contributor.authorCheung, Men_HK
dc.contributor.authorGaviro, MVGen_HK
dc.contributor.authorBooth, Sen_HK
dc.contributor.authorGao, Ben_HK
dc.contributor.authorCheah, KSEen_HK
dc.contributor.authorLovellBadge, Ren_HK
dc.contributor.authorBriscoe, Jen_HK
dc.date.accessioned2011-05-24T02:11:18Z-
dc.date.available2011-05-24T02:11:18Z-
dc.date.issued2010en_HK
dc.identifier.citationNature Neuroscience, 2010, v. 13 n. 10, p. 1181-1189en_HK
dc.identifier.issn1097-6256en_HK
dc.identifier.urihttp://hdl.handle.net/10722/133582-
dc.description.abstractNeural stem cells (NSCs) are uncommitted cells of the CNS defined by their multipotentiality and ability to self renew. We found these cells to not be present in substantial numbers in the CNS until after embryonic day (E) 10.5 in mouse and E5 in chick. This coincides with the induction of SOX9 in neural cells. Gain-and loss-of-function studies indicated that SOX9 was essential for multipotent NSC formation. Moreover, Sonic Hedgehog was able to stimulate precocious generation of NSCs by inducing Sox9 expression. SOX9 was also necessary for the maintenance of multipotent NSCs, as shown by in vivo fate mapping experiments in the adult subependymal zone and olfactory bulbs. In addition, loss of SOX9 led ependymal cells to adopt a neuroblast identity. These data identify a functional link between extrinsic and intrinsic mechanisms of NSCs specification and maintenance, and establish a central role for SOX9 in the process. © 2010 Nature America, Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/neuro/en_HK
dc.relation.ispartofNature Neuroscienceen_HK
dc.subject.meshCell Differentiation - genetics-
dc.subject.meshGene Expression Regulation, Enzymologic - genetics-
dc.subject.meshNeurons - physiology-
dc.subject.meshSOX9 Transcription Factor - genetics - metabolism-
dc.subject.meshStem Cells - physiology-
dc.titleSOX9 induces and maintains neural stem cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1097-6256&volume=13&spage=1181&epage=1189&date=2010&atitle=SOX9+induces+and+maintains+neural+stem+cells-
dc.identifier.emailCheung, M:mcheung9@hkucc.hku.hken_HK
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_HK
dc.identifier.authorityCheung, M=rp00245en_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/nn.2646en_HK
dc.identifier.pmid20871603-
dc.identifier.scopuseid_2-s2.0-77957285454en_HK
dc.identifier.hkuros185430en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77957285454&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1181en_HK
dc.identifier.epage1189en_HK
dc.identifier.eissn1546-1726-
dc.identifier.isiWOS:000282171300009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f10007756960-
dc.relation.projectDevelopmental genomics and skeletal research-
dc.identifier.scopusauthoridScott, CE=36612283000en_HK
dc.identifier.scopusauthoridWynn, SL=36464623600en_HK
dc.identifier.scopusauthoridSesay, A=7006683404en_HK
dc.identifier.scopusauthoridCruz, C=36196411200en_HK
dc.identifier.scopusauthoridCheung, M=7201897461en_HK
dc.identifier.scopusauthoridGaviro, MVG=36491560800en_HK
dc.identifier.scopusauthoridBooth, S=36611315900en_HK
dc.identifier.scopusauthoridGao, B=24481275100en_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK
dc.identifier.scopusauthoridLovellBadge, R=35237977400en_HK
dc.identifier.scopusauthoridBriscoe, J=7005150612en_HK
dc.identifier.citeulike7927159-
dc.identifier.issnl1097-6256-

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