File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Photodynamic action of LED-activated nanoscale photosensitizer in nasopharyngeal carcinoma cells

TitlePhotodynamic action of LED-activated nanoscale photosensitizer in nasopharyngeal carcinoma cells
Authors
KeywordsActive caspase-3
Apoptosis
Apoptotic
Caspase-3
Cellular uptake
Issue Date2010
PublisherM A I K Nauka - Interperiodica. The Journal's web site is located at http://www.maik.ru/journals/lasphys.htm
Citation
Laser Physics, 2010, v. 20 n. 2, p. 544-550 How to Cite?
AbstractBackground: Photodynamic therapy has been confirmed to an efficient therapeutic modality of malignant tumors. The aim of the present study was to explore the photodynamic action of LED-activated nanoscale photosensitizer- loading hypocrellin in nasopharyngeal carcinoma cells. Material/Methods: Nasopharyngeal carcinoma cell line CNE2 cells were subjected to photodynamic therapy with hypocrellinloaded nanophotosensitizer. The uptake of the nanophotosensitizer in the CNE-2 cells was measured using a spectrophotometer and photodynamic toxicity was investigated 18 h after LED radiation treatment. Apoptosis was determined using flow cytometry with propidum iodine staining, and nuclear staining with Hoechst 33258. Active caspase-3 in the CNE2 cells was evaluated using flow cytometry with phycoerythrin (PE)-conjugated anti-active caspase-3 antibodies. Results: The cellular uptake of the nanophotosensitizer in the CNE-2 cells reached optimal at 6 h. LED-activated nanophotosensitizer resulted in doseand light-dependent phototoxicity. Apoptotic rate 18 h after PDT increased to 34.32 ± 1.94% under the light energy of 1 J/cm2. Hoechest 33258 staining reinforced the findings above. Condensation of chromatin and nuclear fragmentations was found in many PDT-treated cells. The activated caspase-3 in the CNE2 cells significantly increased up to 43.90% when the CNE2 cells were exposed to the nanophotosensitizer for 6 h and then 1 J/cm2 irradiation. Conclusion: LED-activated nanophotosensitizer significantly killed the CNE2 cells and enhanced apoptosis and activated caspase-3 in the CNE2 cells. The hypocrellin-loaded nanophotosensitizer might be efficient photosensitizer and LED-activated nanophotosensitizer can be developed for treating nasopharyngeal carcinoma.
Persistent Identifierhttp://hdl.handle.net/10722/131076
ISSN
2023 Impact Factor: 1.2
2023 SCImago Journal Rankings: 0.291
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Polytechnic University
Joint Supervision Scheme with Mainland China UniversitesG-U505
Hi-Tech Research and Development Program of China2006AA02Z4F0
Funding Information:

This work was supported by the Hong Kong Polytechnic University, Joint Supervision Scheme with Mainland China Universites (G-U505) and in part by Hi-Tech Research and Development Program of China (2006AA02Z4F0).

 

DC FieldValueLanguage
dc.contributor.authorBai, DQ-
dc.contributor.authorYow, CMN-
dc.contributor.authorTan, Y-
dc.contributor.authorChu, ESM-
dc.contributor.authorXu, CS-
dc.date.accessioned2011-01-18T06:21:12Z-
dc.date.available2011-01-18T06:21:12Z-
dc.date.issued2010-
dc.identifier.citationLaser Physics, 2010, v. 20 n. 2, p. 544-550-
dc.identifier.issn1054-660X-
dc.identifier.urihttp://hdl.handle.net/10722/131076-
dc.description.abstractBackground: Photodynamic therapy has been confirmed to an efficient therapeutic modality of malignant tumors. The aim of the present study was to explore the photodynamic action of LED-activated nanoscale photosensitizer- loading hypocrellin in nasopharyngeal carcinoma cells. Material/Methods: Nasopharyngeal carcinoma cell line CNE2 cells were subjected to photodynamic therapy with hypocrellinloaded nanophotosensitizer. The uptake of the nanophotosensitizer in the CNE-2 cells was measured using a spectrophotometer and photodynamic toxicity was investigated 18 h after LED radiation treatment. Apoptosis was determined using flow cytometry with propidum iodine staining, and nuclear staining with Hoechst 33258. Active caspase-3 in the CNE2 cells was evaluated using flow cytometry with phycoerythrin (PE)-conjugated anti-active caspase-3 antibodies. Results: The cellular uptake of the nanophotosensitizer in the CNE-2 cells reached optimal at 6 h. LED-activated nanophotosensitizer resulted in doseand light-dependent phototoxicity. Apoptotic rate 18 h after PDT increased to 34.32 ± 1.94% under the light energy of 1 J/cm<sup>2</sup>. Hoechest 33258 staining reinforced the findings above. Condensation of chromatin and nuclear fragmentations was found in many PDT-treated cells. The activated caspase-3 in the CNE2 cells significantly increased up to 43.90% when the CNE2 cells were exposed to the nanophotosensitizer for 6 h and then 1 J/cm<sup>2</sup> irradiation. Conclusion: LED-activated nanophotosensitizer significantly killed the CNE2 cells and enhanced apoptosis and activated caspase-3 in the CNE2 cells. The hypocrellin-loaded nanophotosensitizer might be efficient photosensitizer and LED-activated nanophotosensitizer can be developed for treating nasopharyngeal carcinoma.-
dc.languageeng-
dc.publisherM A I K Nauka - Interperiodica. The Journal's web site is located at http://www.maik.ru/journals/lasphys.htm-
dc.relation.ispartofLaser Physics-
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectActive caspase-3-
dc.subjectApoptosis-
dc.subjectApoptotic-
dc.subjectCaspase-3-
dc.subjectCellular uptake-
dc.titlePhotodynamic action of LED-activated nanoscale photosensitizer in nasopharyngeal carcinoma cellsen_US
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1054-660X&volume=20&issue=2&spage=544&epage=550&date=2010&atitle=Photodynamic+action+of+LED-activated+nanoscale+photosensitizer+in+nasopharyngeal+carcinoma+cells-
dc.identifier.emailYow, CMN: Christine.Yow@inet.polyu.edu.hk-
dc.identifier.emailChu, ESM: elliecsm@hku.hk, htellie@inet.polyu.edu.hk-
dc.identifier.emailXu, CS: xcshan@163.com-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1134/S1054660X10030011-
dc.identifier.scopuseid_2-s2.0-77649275065-
dc.identifier.hkuros174522-
dc.identifier.volume20-
dc.identifier.issue2-
dc.identifier.spage544-
dc.identifier.epage550-
dc.identifier.isiWOS:000276063200035-
dc.identifier.citeulike6501861-
dc.identifier.issnl1054-660X-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats