Human gingiva is another site of C-reactive protein formation

Abstract

Objectives: C-reactive protein (CRP) is primarily synthesized in the liver. It is hypothesized that human gingiva per se may produce CRP and its expression could be associated with IL-6. This study elucidated the CRP expression profile in human gingiva and its possible association with IL-6. Materials and Methods: Ninety-four gingival biopsies were collected from 44 subjects with chronic periodontitis and 18 periodontally healthy subjects. CRP protein was detected by immunohistochemistry and Western blotting, while CRP and IL-6 mRNAs were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR. CRP protein expression in the reconstituted human gingival epithelia (RHGE) was examined by the particle-enhanced immunoturbidimetric assay and Western blotting. Results: CRP protein was detected in gingival tissues from patients and healthy subjects by immunohistochemistry and confirmed by Western blotting. Its expression pattern and level at 16 pairs of periodontal pocket tissues and the adjacent clinically healthy tissues from 16 patients were significantly interrelated (rs=0.693, p<0.01). CRP mRNA expression was strongly correlated with IL-6 (r=0.694, p<0.001). Both CRP protein and mRNA were detected in the RHGE. Conclusions: The present study shows for the first time that human gingiva is able to produce CRP in situ that may be associated with IL-6 activity. © 2010 John Wiley & Sons A/S.