Extraction of Total Alkaloids from Corydalis saxicola Bunting by the Macroporous Resin

Abstract

The total alkaloids were extracted from Corydalis saxicola Bunting by macroporous resin.Six different macroporous resins were used for extraction of the total alkaloids and dehydrocavidine by using static and dynamic adsorption-desorption experiments.The specific adsorption capacity and eluting of D101 macroporous resin were comparatively high.The optimum parameters of the purification process were established as the sample concentration of 0.2 g/ml at the absorption ? ow rate of 2 BV/h and with 3 BV of 60% ethanol as the desorption solvent.The contents of total alkaloids in the purifi ed extract were over 60%.

利用大孔吸附樹脂分離巖黃連中的總生物堿,采用靜態和動態吸附-解吸附方法,以總生物堿和脫氫卡維丁含量為指標,考察了6種大孔吸附樹脂對巖黃連總生物堿的吸附能力,其中D101型大孔吸附樹脂的比吸附量和比洗脫量均較高。優化后的提取工藝為:上樣藥液原藥材濃度0.2g/ml,吸附流速為2BV/h,解吸附溶劑為60%乙醇(3BV),所得巖黃連提取物中總生物堿含量大于60%。