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Conference Paper: Growth suppression activity of tensin2 in human hepatocellular carcinoma is dependent on PTEN and SH2 domains

TitleGrowth suppression activity of tensin2 in human hepatocellular carcinoma is dependent on PTEN and SH2 domains
Authors
Issue Date2010
PublisherElsevier BV. The Journal's web site is located at www.ejcancer.info/supplements
Citation
The 21st Meeting of the European Association for Cancer Research (EACR 2010), Oslo, Norway, 26-29 June 2010. In European Journal of Cancer Supplements, 2010, v. 8 n. 5, p. 171, abstract no. 677 How to Cite?
AbstractBACKGROUND: Tensin is a group of focal adhesion proteins that serves as a link between cytoskeleton and signal transduction. Dysregulation of tensin members have been revealed in various human cancers, including hepatocellular carcinoma (HCC). Our previous study has shown that tensin2 exerted pronounced cell death in HCC cells and was downregulated in 41% of human HCCs. In this study, we functionally characterized the role of tensin2 in HCC. MATERIALS AND METHODS: Functional characterization of tensin2 was studied by stable overexpression or knockdown of tensin2 in HCC cells with lentiviral delivery system. The proliferation rate, migration and invasion ability of the stable clones were monitored by growth curve, transwell assay and matrigel invasion assay, respectively. The in vivo effect of tensin2 in HCC tumour formation was studied in nude mice. Tensin2 knockdown stable clones were subcutaneously injected into nude mice and tumour growth was monitored for 4 weeks. Tensin2 deletion mutants were expressed in HCC cells and their apoptotic inducing activities were analyzed by flow cytometry and TUNEL assay. RESULTS: Tensin2 overexpression stable clones displayed lower proliferation rate, decreased anchorage-independent growth, inhibited motility and invasiveness when compared with the vector control. Conversely, stable knockdown clones of tensin2 showed higher proliferation rate, increased motility and invasiveness. Enhanced tumour formation in nude mice was also observed in stable knockdown clones. Transient expression of tensin2 induced significant suppression in colony formation of HCC cells. However, the suppression effect was lost in tensin2 mutants with either PTEN or SH2 domain deleted. TUNEL assay revealed that the number of apoptotic cells was inversely correlated with the number of HCC colonies formed. CONCLUSIONS: Our study showed that tensin2 plays a negative regulatory role in HCC development and revealed the biological significance of the PTEN and SH2 domains in the growth suppression activity of tensin2.
DescriptionPoster Session: Molecular Biology
Persistent Identifierhttp://hdl.handle.net/10722/126694
ISSN
2010 Impact Factor: 9.386
2023 SCImago Journal Rankings: 0.759
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYeung, YSen_HK
dc.contributor.authorTse, EYTen_HK
dc.contributor.authorKo, FCFen_HK
dc.contributor.authorChan, LKen_HK
dc.contributor.authorSze, KMFen_HK
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorYam, JWPen_HK
dc.date.accessioned2010-10-31T12:43:08Z-
dc.date.available2010-10-31T12:43:08Z-
dc.date.issued2010en_HK
dc.identifier.citationThe 21st Meeting of the European Association for Cancer Research (EACR 2010), Oslo, Norway, 26-29 June 2010. In European Journal of Cancer Supplements, 2010, v. 8 n. 5, p. 171, abstract no. 677en_HK
dc.identifier.issn1359-6349-
dc.identifier.urihttp://hdl.handle.net/10722/126694-
dc.descriptionPoster Session: Molecular Biology-
dc.description.abstractBACKGROUND: Tensin is a group of focal adhesion proteins that serves as a link between cytoskeleton and signal transduction. Dysregulation of tensin members have been revealed in various human cancers, including hepatocellular carcinoma (HCC). Our previous study has shown that tensin2 exerted pronounced cell death in HCC cells and was downregulated in 41% of human HCCs. In this study, we functionally characterized the role of tensin2 in HCC. MATERIALS AND METHODS: Functional characterization of tensin2 was studied by stable overexpression or knockdown of tensin2 in HCC cells with lentiviral delivery system. The proliferation rate, migration and invasion ability of the stable clones were monitored by growth curve, transwell assay and matrigel invasion assay, respectively. The in vivo effect of tensin2 in HCC tumour formation was studied in nude mice. Tensin2 knockdown stable clones were subcutaneously injected into nude mice and tumour growth was monitored for 4 weeks. Tensin2 deletion mutants were expressed in HCC cells and their apoptotic inducing activities were analyzed by flow cytometry and TUNEL assay. RESULTS: Tensin2 overexpression stable clones displayed lower proliferation rate, decreased anchorage-independent growth, inhibited motility and invasiveness when compared with the vector control. Conversely, stable knockdown clones of tensin2 showed higher proliferation rate, increased motility and invasiveness. Enhanced tumour formation in nude mice was also observed in stable knockdown clones. Transient expression of tensin2 induced significant suppression in colony formation of HCC cells. However, the suppression effect was lost in tensin2 mutants with either PTEN or SH2 domain deleted. TUNEL assay revealed that the number of apoptotic cells was inversely correlated with the number of HCC colonies formed. CONCLUSIONS: Our study showed that tensin2 plays a negative regulatory role in HCC development and revealed the biological significance of the PTEN and SH2 domains in the growth suppression activity of tensin2.-
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at www.ejcancer.info/supplements-
dc.relation.ispartofEuropean Journal of Cancer Supplements-
dc.titleGrowth suppression activity of tensin2 in human hepatocellular carcinoma is dependent on PTEN and SH2 domainsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1359-6349&volume=8&issue=5&spage=171, abstract no. 677&epage=&date=2010&atitle=Growth+suppression+activity+of+tensin2+in+human+hepatocellular+carcinoma+is+dependent+on+PTEN+and+SH2+domains-
dc.identifier.emailYeung, YS: yeungys@hkucc.hku.hken_HK
dc.identifier.emailKo, FCF: bokcf@hkucc.hku.hken_HK
dc.identifier.emailChan, LK: lokongchan@gmail.comen_HK
dc.identifier.emailSze, KMF: szekaren@yahoo.comen_HK
dc.identifier.emailNg, IOL: iolng@hkucc.hku.hken_HK
dc.identifier.emailYam, JWP: judyyam@pathology.hku.hken_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.identifier.authorityYam, JWP=rp00468en_HK
dc.identifier.doi10.1016/S1359-6349(10)71474-9-
dc.identifier.hkuros172260en_HK
dc.identifier.volume8-
dc.identifier.issue5-
dc.identifier.spage171, abstract no. 677-
dc.identifier.epage171, abstract no. 677-
dc.identifier.isiWOS:000288603100652-
dc.description.otherThe 21st Meeting of the European Association for Cancer Research (EACR), Oslo, Norway, 26-29 June 2010. In European Journal of Cancer Supplements, 2010, v. 8 n. 5, p. 171, abstract no. 677-
dc.identifier.issnl1359-6349-

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