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- Publisher Website: 10.1080/09674845.2010.11730296
- Scopus: eid_2-s2.0-77954304391
- PMID: 20669764
- WOS: WOS:000278893000005
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Article: Evaluation of in-house and commercial genotyping assays for molecular typing of hepatitis C virus in Hong Kong
Title | Evaluation of in-house and commercial genotyping assays for molecular typing of hepatitis C virus in Hong Kong |
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Authors | |
Keywords | Genotype Hepatitis C Hybridization, genetic Polymerase chain reaction |
Issue Date | 2010 |
Publisher | Step Communications Ltd. The Journal's web site is located at http://www.ibms.org/index.cfm?method=publications.british_journal |
Citation | British Journal Of Biomedical Science, 2010, v. 67 n. 2, p. 82-85 How to Cite? |
Abstract | This study aims to evaluate genotyping assays for hepatitis C virus (HCV). An in-house nucleic acid sequencing method is performed in parallel with the Roche Linear Array HCV genotyping test on 73 HCV-positive (66 clinical samples and seven proficiency testing quality control samples) and 12 HCV-negative samples (11 clinical samples and one proficiency testing sample). The performance of the in-house method was comparable with that of the Roche assay (concordance rate: 89.4%). Discordant results included four mixed infections missed by the in-house method, two false-negatives with the Roche assay, and three discrepant results. The in-house method exhibited a higher resolution (subtype vs. genotype level) at a lower running cost (25% of the commercial assay). The in-house method was also used to genotype 375 HCV clinical isolates to determine the genotypic distribution of HCV in Hong Kong between 2005 and 2008. A total of 441 (52.8%) clinical isolates proved to be genotype 1, which shows a poorer response to interferon therapy. Genotype 6 was the next most common (32.0%). Prevalence of genotypes 2 and 3 was 7.7% and 6.6%, respectively, and prevalence of genotypes 4 and 5 was 0.9% and 0%, respectively. Although the in-house nucleic acid sequencing method failed to detect a few cases of mixed HCV infection, its high resolution and low running cost make it suitable for surveillance and outbreak investigation. |
Persistent Identifier | http://hdl.handle.net/10722/125111 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.498 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lam, THJ | en_HK |
dc.contributor.author | Cheng, PS | en_HK |
dc.contributor.author | Lai, ST | en_HK |
dc.contributor.author | Tsang, TY | en_HK |
dc.contributor.author | Cheng, VCC | en_HK |
dc.contributor.author | Ho, SL | en_HK |
dc.contributor.author | Yam, WC | en_HK |
dc.date.accessioned | 2010-10-31T11:12:00Z | - |
dc.date.available | 2010-10-31T11:12:00Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | British Journal Of Biomedical Science, 2010, v. 67 n. 2, p. 82-85 | en_HK |
dc.identifier.issn | 0967-4845 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/125111 | - |
dc.description.abstract | This study aims to evaluate genotyping assays for hepatitis C virus (HCV). An in-house nucleic acid sequencing method is performed in parallel with the Roche Linear Array HCV genotyping test on 73 HCV-positive (66 clinical samples and seven proficiency testing quality control samples) and 12 HCV-negative samples (11 clinical samples and one proficiency testing sample). The performance of the in-house method was comparable with that of the Roche assay (concordance rate: 89.4%). Discordant results included four mixed infections missed by the in-house method, two false-negatives with the Roche assay, and three discrepant results. The in-house method exhibited a higher resolution (subtype vs. genotype level) at a lower running cost (25% of the commercial assay). The in-house method was also used to genotype 375 HCV clinical isolates to determine the genotypic distribution of HCV in Hong Kong between 2005 and 2008. A total of 441 (52.8%) clinical isolates proved to be genotype 1, which shows a poorer response to interferon therapy. Genotype 6 was the next most common (32.0%). Prevalence of genotypes 2 and 3 was 7.7% and 6.6%, respectively, and prevalence of genotypes 4 and 5 was 0.9% and 0%, respectively. Although the in-house nucleic acid sequencing method failed to detect a few cases of mixed HCV infection, its high resolution and low running cost make it suitable for surveillance and outbreak investigation. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Step Communications Ltd. The Journal's web site is located at http://www.ibms.org/index.cfm?method=publications.british_journal | en_HK |
dc.relation.ispartof | British Journal of Biomedical Science | en_HK |
dc.subject | Genotype | en_HK |
dc.subject | Hepatitis C | en_HK |
dc.subject | Hybridization, genetic | en_HK |
dc.subject | Polymerase chain reaction | en_HK |
dc.subject.mesh | Hepacivirus - classification - genetics - isolation and purification | - |
dc.subject.mesh | Hepatitis C - genetics | - |
dc.subject.mesh | Polymerase Chain Reaction - methods | - |
dc.subject.mesh | Predictive Value of Tests | - |
dc.subject.mesh | Sequence Analysis, DNA - methods | - |
dc.title | Evaluation of in-house and commercial genotyping assays for molecular typing of hepatitis C virus in Hong Kong | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Yam, WC:wcyam@hkucc.hku.hk | en_HK |
dc.identifier.authority | Yam, WC=rp00313 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1080/09674845.2010.11730296 | - |
dc.identifier.pmid | 20669764 | - |
dc.identifier.scopus | eid_2-s2.0-77954304391 | en_HK |
dc.identifier.hkuros | 179782 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77954304391&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 67 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 82 | en_HK |
dc.identifier.epage | 85 | en_HK |
dc.identifier.isi | WOS:000278893000005 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Lam, THJ=54792600300 | en_HK |
dc.identifier.scopusauthorid | Cheng, PS=7401619029 | en_HK |
dc.identifier.scopusauthorid | Lai, ST=7402937038 | en_HK |
dc.identifier.scopusauthorid | Tsang, TY=7101832362 | en_HK |
dc.identifier.scopusauthorid | Cheng, VCC=23670479400 | en_HK |
dc.identifier.scopusauthorid | Ho, SL=55041381700 | en_HK |
dc.identifier.scopusauthorid | Yam, WC=7004281720 | en_HK |
dc.identifier.issnl | 0967-4845 | - |