File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1002/ejlt.201000018
- Scopus: eid_2-s2.0-77956905976
- WOS: WOS:000282662800016
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Analysis of mitochondria by capillary electrophoresis: Cardiolipin levels decrease in response to carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone
Title | Analysis of mitochondria by capillary electrophoresis: Cardiolipin levels decrease in response to carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone | ||||||
---|---|---|---|---|---|---|---|
Authors | |||||||
Keywords | Capillary electrophoresis Cardiolipin FCCP Mitochondria | ||||||
Issue Date | 2010 | ||||||
Publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/69502350 | ||||||
Citation | European Journal Of Lipid Science And Technology, 2010, v. 112 n. 9, p. 1058-1066 How to Cite? | ||||||
Abstract | Cardiolipin is an important phospholipid present in the inner membrane of mitochondria. It plays a critical role in adenosine triphosphate (ATP) synthesis mediated by oxidative phosphorylation. Exposure of HepG2 cells to carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) caused the inhibition of ATP synthesis and the depolarization of mitochondria. Capillary electrophoresis with laser-induced fluorescence (CE-LIF) analysis of fluorescent mitochondrion-selective probe 10-N-nonyl acridine orange (NAO) labeled mitochondria was employed to in situ estimate the cardiolipin levels under FCCP-induced de-energization of mitochondria. NAO, stoichiometriclly bound to cardiolipin at a 1:1 or 2:1 molar ratio (NAO/cardiolipin), emitted green and red fluorescence, respectively. Green fluorescence was chosen for cardiolipin content analysis because it was more intense than red fluorescence. A significant decrease in the cardiolipin content, up to 11% of the control, was evident when the ATP content and mitochondrial membrane potential (MMP) correspondingly decreased. These related findings suggested that CE-LIF may provide a sensitive strategy to determine cardiolipin content in response to exposure to chemical uncouplers. This reinforces the hypothesis that alterations in ATP synthesis and MMP have a close association with cardiolipin content, which correlated tightly with mitochondrial membrane assembly and activity. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/124494 | ||||||
ISSN | 2023 Impact Factor: 1.8 2023 SCImago Journal Rankings: 0.475 | ||||||
ISI Accession Number ID |
Funding Information: We would like to thank Dr. Wilson Ching Yickpang for kindly providing HepG2 cells and the support from the Hong Kong Research Grants Council of the Hong Kong Special Administrative Region, China (HKU7023/07P) and the Hong Kong University Research and Conference Grants Committee. | ||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhao, W | en_HK |
dc.contributor.author | O, W | en_HK |
dc.contributor.author | Fung, Y | en_HK |
dc.contributor.author | Cheung, MP | en_HK |
dc.date.accessioned | 2010-10-31T10:37:33Z | - |
dc.date.available | 2010-10-31T10:37:33Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | European Journal Of Lipid Science And Technology, 2010, v. 112 n. 9, p. 1058-1066 | en_HK |
dc.identifier.issn | 1438-7697 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/124494 | - |
dc.description.abstract | Cardiolipin is an important phospholipid present in the inner membrane of mitochondria. It plays a critical role in adenosine triphosphate (ATP) synthesis mediated by oxidative phosphorylation. Exposure of HepG2 cells to carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) caused the inhibition of ATP synthesis and the depolarization of mitochondria. Capillary electrophoresis with laser-induced fluorescence (CE-LIF) analysis of fluorescent mitochondrion-selective probe 10-N-nonyl acridine orange (NAO) labeled mitochondria was employed to in situ estimate the cardiolipin levels under FCCP-induced de-energization of mitochondria. NAO, stoichiometriclly bound to cardiolipin at a 1:1 or 2:1 molar ratio (NAO/cardiolipin), emitted green and red fluorescence, respectively. Green fluorescence was chosen for cardiolipin content analysis because it was more intense than red fluorescence. A significant decrease in the cardiolipin content, up to 11% of the control, was evident when the ATP content and mitochondrial membrane potential (MMP) correspondingly decreased. These related findings suggested that CE-LIF may provide a sensitive strategy to determine cardiolipin content in response to exposure to chemical uncouplers. This reinforces the hypothesis that alterations in ATP synthesis and MMP have a close association with cardiolipin content, which correlated tightly with mitochondrial membrane assembly and activity. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/69502350 | en_HK |
dc.relation.ispartof | European Journal of Lipid Science and Technology | en_HK |
dc.subject | Capillary electrophoresis | en_HK |
dc.subject | Cardiolipin | en_HK |
dc.subject | FCCP | en_HK |
dc.subject | Mitochondria | en_HK |
dc.title | Analysis of mitochondria by capillary electrophoresis: Cardiolipin levels decrease in response to carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1438-7697&volume=112&spage=1058&epage=1066&date=2010&atitle=Analysis+of+mitochondria+by+capillary+electrophoresis:+Cardiolipin+levels+decrease+in+response+to+carbonyl+cyanide+4-(trifluoromethoxy)+phenylhydrazone | en_HK |
dc.identifier.email | O, W:owaisum@hkucc.hku.hk | en_HK |
dc.identifier.email | Fung, Y:ysfung@hku.hk | en_HK |
dc.identifier.authority | O, W=rp00315 | en_HK |
dc.identifier.authority | Fung, Y=rp00697 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/ejlt.201000018 | en_HK |
dc.identifier.scopus | eid_2-s2.0-77956905976 | en_HK |
dc.identifier.hkuros | 182875 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77956905976&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 112 | en_HK |
dc.identifier.issue | 9 | en_HK |
dc.identifier.spage | 1058 | en_HK |
dc.identifier.epage | 1066 | en_HK |
dc.identifier.isi | WOS:000282662800016 | - |
dc.publisher.place | Germany | en_HK |
dc.identifier.scopusauthorid | Zhao, W=34871080300 | en_HK |
dc.identifier.scopusauthorid | O, W=6701729369 | en_HK |
dc.identifier.scopusauthorid | Fung, Y=13309754700 | en_HK |
dc.identifier.scopusauthorid | Cheung, MP=16749051200 | en_HK |
dc.identifier.issnl | 1438-7697 | - |