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Article: Diffusion tensor imaging of renal ischemia reperfusion injury in an experimental model
Title | Diffusion tensor imaging of renal ischemia reperfusion injury in an experimental model | ||||
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Authors | |||||
Keywords | Apparent diffusion coefficient Diffusion tensor imaging Diffusion-weighted imaging Fractional anisotropy Ischemic reperfusion injury IVIM Kidney MRI | ||||
Issue Date | 2010 | ||||
Publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/13087 | ||||
Citation | Nmr In Biomedicine, 2010, v. 23 n. 5, p. 496-502 How to Cite? | ||||
Abstract | Renal ischemia reperfusion injury (IRI) is a major cause of acute renal failure. It occurs in various clinical settings such as renal transplantation, shock and vascular surgery. Serum creatinine level has been used as an index for estimating the degree of renal functional loss in renal IRI. However, it only evaluates the global renal function. In this study, diffusion tensor imaging (DTI) was used to characterize renal IRI in an experimental rat model. Spin-echo echo-planar DTI with b-value of 300 s/mm 2 and 6 diffusion gradient directions was performed at 7 T in 8 Sprague-Dawley (SD) with 60-min unilateral renal IRI and 8 normal SD rats. Apparent diffusion coefficient (ADC), directional diffusivities and fractional anisotropy (FA) were measured at the acute stage of IRI. The IR-injured animals were also examined by diffusion-weighted imaging with 7 b-values up to 1000 s/mm 2 to estimate true diffusion coefficient (D true) and perfusion fraction (P fraction) using a bi-compartmental model. ADC of injured renal cortex (1.69±0.24×10 -3mm 2/s) was significantly lower (p<0.01) than that of contralateral intact cortex (2.03±0.35×10 -3mm 2/s). Meanwhile, both ADC and FA of IR-injured medulla (1.37±0.27×10 -3mm 2/s and 0.28±0.04, respectively) were significantly less (p<0.01) than those of contralateral intact medulla (2.01±0. 38×10 -3mm 2/s and 0.36±0.04, respectively). The bi-compartmental model analysis revealed the decrease in D true and P fraction in the IR-injured kidneys. Kidney histology showed widespread cell swelling and erythrocyte congestion in both cortex and medulla, and cell necrosis/apoptosis and cast formation in medulla. These experimental findings demonstrated that DTI can probe both structural and functional information of kidneys following renal IRI. Copyright © 2010 John Wiley & Sons, Ltd. | ||||
Persistent Identifier | http://hdl.handle.net/10722/123831 | ||||
ISSN | 2021 Impact Factor: 4.478 2020 SCImago Journal Rankings: 1.278 | ||||
ISI Accession Number ID |
Funding Information: Contract/grant sponsor: Hong Kong Research Grant Council; contract/grant number: CERG HKU 7808/09M. | ||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cheung, JS | en_HK |
dc.contributor.author | Fan, SJ | en_HK |
dc.contributor.author | Chow, AM | en_HK |
dc.contributor.author | Zhang, J | en_HK |
dc.contributor.author | Man, K | en_HK |
dc.contributor.author | Wu, EX | en_HK |
dc.date.accessioned | 2010-09-30T03:15:10Z | - |
dc.date.available | 2010-09-30T03:15:10Z | - |
dc.date.issued | 2010 | en_HK |
dc.identifier.citation | Nmr In Biomedicine, 2010, v. 23 n. 5, p. 496-502 | en_HK |
dc.identifier.issn | 0952-3480 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/123831 | - |
dc.description.abstract | Renal ischemia reperfusion injury (IRI) is a major cause of acute renal failure. It occurs in various clinical settings such as renal transplantation, shock and vascular surgery. Serum creatinine level has been used as an index for estimating the degree of renal functional loss in renal IRI. However, it only evaluates the global renal function. In this study, diffusion tensor imaging (DTI) was used to characterize renal IRI in an experimental rat model. Spin-echo echo-planar DTI with b-value of 300 s/mm 2 and 6 diffusion gradient directions was performed at 7 T in 8 Sprague-Dawley (SD) with 60-min unilateral renal IRI and 8 normal SD rats. Apparent diffusion coefficient (ADC), directional diffusivities and fractional anisotropy (FA) were measured at the acute stage of IRI. The IR-injured animals were also examined by diffusion-weighted imaging with 7 b-values up to 1000 s/mm 2 to estimate true diffusion coefficient (D true) and perfusion fraction (P fraction) using a bi-compartmental model. ADC of injured renal cortex (1.69±0.24×10 -3mm 2/s) was significantly lower (p<0.01) than that of contralateral intact cortex (2.03±0.35×10 -3mm 2/s). Meanwhile, both ADC and FA of IR-injured medulla (1.37±0.27×10 -3mm 2/s and 0.28±0.04, respectively) were significantly less (p<0.01) than those of contralateral intact medulla (2.01±0. 38×10 -3mm 2/s and 0.36±0.04, respectively). The bi-compartmental model analysis revealed the decrease in D true and P fraction in the IR-injured kidneys. Kidney histology showed widespread cell swelling and erythrocyte congestion in both cortex and medulla, and cell necrosis/apoptosis and cast formation in medulla. These experimental findings demonstrated that DTI can probe both structural and functional information of kidneys following renal IRI. Copyright © 2010 John Wiley & Sons, Ltd. | en_HK |
dc.language | eng | - |
dc.publisher | John Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/13087 | en_HK |
dc.relation.ispartof | NMR in Biomedicine | en_HK |
dc.rights | N M R in Biomedicine. Copyright © John Wiley & Sons Ltd. | - |
dc.subject | Apparent diffusion coefficient | en_HK |
dc.subject | Diffusion tensor imaging | en_HK |
dc.subject | Diffusion-weighted imaging | en_HK |
dc.subject | Fractional anisotropy | en_HK |
dc.subject | Ischemic reperfusion injury | en_HK |
dc.subject | IVIM | en_HK |
dc.subject | Kidney | en_HK |
dc.subject | MRI | en_HK |
dc.title | Diffusion tensor imaging of renal ischemia reperfusion injury in an experimental model | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0952-3480&volume=23&issue=5&spage=496&epage=502&date=2010&atitle=Diffusion+tensor+imaging+of+renal+ischemia+reperfusion+injury+in+an+experimental+model | - |
dc.identifier.email | Man, K: kwanman@hkucc.hku.hk | en_HK |
dc.identifier.email | Wu, EX: ewu1@hkucc.hku.hk | en_HK |
dc.identifier.authority | Man, K=rp00417 | en_HK |
dc.identifier.authority | Wu, EX=rp00193 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/nbm.1486 | en_HK |
dc.identifier.pmid | 20175152 | - |
dc.identifier.scopus | eid_2-s2.0-77953501951 | en_HK |
dc.identifier.hkuros | 173547 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-77953501951&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 23 | en_HK |
dc.identifier.issue | 5 | en_HK |
dc.identifier.spage | 496 | en_HK |
dc.identifier.epage | 502 | en_HK |
dc.identifier.isi | WOS:000279526300008 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Cheung, JS=16174280400 | en_HK |
dc.identifier.scopusauthorid | Fan, SJ=36514618100 | en_HK |
dc.identifier.scopusauthorid | Chow, AM=16174234200 | en_HK |
dc.identifier.scopusauthorid | Zhang, J=15752209000 | en_HK |
dc.identifier.scopusauthorid | Man, K=7101754072 | en_HK |
dc.identifier.scopusauthorid | Wu, EX=7202128034 | en_HK |
dc.identifier.issnl | 0952-3480 | - |