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Article: Formation of virus-like particles from human cell lines exclusively expressing influenza neuraminidase

TitleFormation of virus-like particles from human cell lines exclusively expressing influenza neuraminidase
Authors
Lai, JCCChan, WWLKien, FNicholls, JMPeiris, JSMGarcia, JM
Issue Date2010
PublisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.org
Citation
Journal Of General Virology, 2010, v. 91 n. 9, p. 2322-2330 How to Cite?
DOI: http://dx.doi.org/10.1099/vir.0.019935-0
AbstractThe minimal virus requirements for the generation of influenza virus-like particle (VLP) assembly and budding were reassessed. Using neuraminidase (NA) from the H5N1 and H1N1 subtypes, it was found that the expression of NA alone was sufficient to generate and release VLPs. Biochemical and functional characterization of the NA-containing VLPs demonstrated that they were morphologically similar to influenza virions. The NA oligomerization was comparable to that of the live virus, and the enzymic activity, whilst not required for the release of NA-VLPs, was preserved. Together, these findings indicate that NA plays a key role in virus budding and morphogenesis, and demonstrate that NA-VLPs represent a useful tool in influenza research. © 2010 SGM.
Persistent Identifierhttp://hdl.handle.net/10722/123807
ISSN
0022-1317
2021 Impact Factor: 5.141
2020 SCImago Journal Rankings: 1.550
ISI Accession Number ID
WOS:000282147300021
Funding AgencyGrant Number
University Grants CommitteeAoE/M-12/-06
HKU 10208924
HKU 4774109
French Ministry of Health
RESPARI Pasteur network
Li Ka Shing Foundation
Funding Information:

This work was supported by the Area of Excellence Scheme of the University Grants Committee (grant AoE/M-12/-06 of the Hong Kong Special Administrative Region, Hong Kong SAR), the University Grants Committee project HKU 10208924 and HKU 4774109, the French Ministry of Health, RESPARI Pasteur network, and the Li Ka Shing Foundation. We would like to thank Dr K. Y. Hui (Department of Microbiology, University of Hong Kong) for carrying out the Biosafety Level 3 work on H5N1.

References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorLai, JCCen_HK
dc.contributor.authorChan, WWLen_HK
dc.contributor.authorKien, Fen_HK
dc.contributor.authorNicholls, JMen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorGarcia, JMen_HK
dc.date.accessioned2010-09-27T07:10:49Z-
dc.date.available2010-09-27T07:10:49Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of General Virology, 2010, v. 91 n. 9, p. 2322-2330en_HK
dc.identifier.issn0022-1317en_HK
dc.identifier.urihttp://hdl.handle.net/10722/123807-
dc.description.abstractThe minimal virus requirements for the generation of influenza virus-like particle (VLP) assembly and budding were reassessed. Using neuraminidase (NA) from the H5N1 and H1N1 subtypes, it was found that the expression of NA alone was sufficient to generate and release VLPs. Biochemical and functional characterization of the NA-containing VLPs demonstrated that they were morphologically similar to influenza virions. The NA oligomerization was comparable to that of the live virus, and the enzymic activity, whilst not required for the release of NA-VLPs, was preserved. Together, these findings indicate that NA plays a key role in virus budding and morphogenesis, and demonstrate that NA-VLPs represent a useful tool in influenza research. © 2010 SGM.en_HK
dc.languageeng-
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://vir.sgmjournals.orgen_HK
dc.relation.ispartofJournal of General Virologyen_HK
dc.rightsJournal of General Virology. Copyright © Society for General Microbiology.-
dc.subject.meshInfluenza A Virus, H1N1 Subtype - genetics - physiology - ultrastructure-
dc.subject.meshInfluenza A Virus, H5N1 Subtype - genetics - physiology - ultrastructure-
dc.subject.meshNeuraminidase - genetics - physiology-
dc.subject.meshViral Proteins - genetics - physiology-
dc.subject.meshVirus Assembly - genetics - physiology-
dc.titleFormation of virus-like particles from human cell lines exclusively expressing influenza neuraminidaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1317&volume=91&issue=9&spage=2322&epage=2330&date=2010&atitle=Formation+of+virus-like+particles+from+human+cell+lines+exclusively+expressing+Influenza+neuraminidase-
dc.identifier.emailNicholls, JM: jmnichol@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityNicholls, JM=rp00364en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1099/vir.0.019935-0en_HK
dc.identifier.pmid20505010-
dc.identifier.scopuseid_2-s2.0-77956270840en_HK
dc.identifier.hkuros172046-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956270840&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume91en_HK
dc.identifier.issue9en_HK
dc.identifier.spage2322en_HK
dc.identifier.epage2330en_HK
dc.identifier.isiWOS:000282147300021-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLai, JCC=24066794100en_HK
dc.identifier.scopusauthoridChan, WWL=36629918800en_HK
dc.identifier.scopusauthoridKien, F=7004231633en_HK
dc.identifier.scopusauthoridNicholls, JM=7201463077en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridGarcia, JM=16202601400en_HK
dc.identifier.citeulike11130933-
dc.identifier.issnl0022-1317-

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