File Download
There are no files associated with this item.
Supplementary
-
Citations:
- Appears in Collections:
Conference Paper: Distributions of alpha 2 adrenoceptor subtypes in porcine coronary vasculature
Title | Distributions of alpha 2 adrenoceptor subtypes in porcine coronary vasculature |
---|---|
Authors | |
Issue Date | 2008 |
Publisher | Federation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl |
Citation | The 2008 Annual Meeting of Experimental Biology (EB 2008) - ASPET's Centennial Meeting, San Diego, CA., 5-9 April 2008. In The FASEB Journal, 2008, v. 22 meeting abstract suppl., abstract no. 914.10 How to Cite? |
Abstract | Alpha 2-adrenoreceptor agonists affect vascular tone of coronary vasculature by multiple mechanisms. One of the mechanisms is the direct action on endothelial {alpha}2 adrenoreceptors (mainly {alpha}2A), which induces an endothelial-dependent vasorelaxation via release of nitric oxide. We sought to investigate the subtypes of {alpha}2 adrenoceptors and their relative abundance in coronary blood vessels of different sizes (arteries vs arterioles). Result of RT-PCR clearly demonstrated that mRNA of {alpha}2A and {alpha}2B adrenoceptors was present in coronary arteries but mRNA of {alpha}2C-adrenoceptors was absent. In contrast, mRNA of {alpha}2B and {alpha}2C adrenoceptors was present in subendocardial myocardium (which contains coronary arterioles) but mRNA of {alpha}2A adrenoceptors was not. While mRNA of {alpha}2B adrenoceptors were detected in both coronary arteries and subendocardial myocardium, quantitative analysis revealed that its expression was higher in coronary arteries. Immunohistochemical studies showed that {alpha}2A and {alpha}2B adrenoceptor proteins were located mainly on the endothelial cells of coronary arteries but none was visualized in coronary arterioles. Distributions and cellular localizations of {alpha}2C adrenoceptor proteins had not yet been studied since specific antibody was not available. This preliminary data suggests that coronary blood vessels of different sizes may respond differently to pharmacological agents. |
Persistent Identifier | http://hdl.handle.net/10722/112812 |
ISSN | 2021 Impact Factor: 5.834 2020 SCImago Journal Rankings: 1.709 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ho, YW | en_HK |
dc.contributor.author | Leung, GPH | en_HK |
dc.contributor.author | Man, RYK | en_HK |
dc.contributor.author | Vanhoutte, PMGR | en_HK |
dc.contributor.author | Ng, JKF | en_HK |
dc.date.accessioned | 2010-09-26T03:48:18Z | - |
dc.date.available | 2010-09-26T03:48:18Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | The 2008 Annual Meeting of Experimental Biology (EB 2008) - ASPET's Centennial Meeting, San Diego, CA., 5-9 April 2008. In The FASEB Journal, 2008, v. 22 meeting abstract suppl., abstract no. 914.10 | - |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | http://hdl.handle.net/10722/112812 | - |
dc.description.abstract | Alpha 2-adrenoreceptor agonists affect vascular tone of coronary vasculature by multiple mechanisms. One of the mechanisms is the direct action on endothelial {alpha}2 adrenoreceptors (mainly {alpha}2A), which induces an endothelial-dependent vasorelaxation via release of nitric oxide. We sought to investigate the subtypes of {alpha}2 adrenoceptors and their relative abundance in coronary blood vessels of different sizes (arteries vs arterioles). Result of RT-PCR clearly demonstrated that mRNA of {alpha}2A and {alpha}2B adrenoceptors was present in coronary arteries but mRNA of {alpha}2C-adrenoceptors was absent. In contrast, mRNA of {alpha}2B and {alpha}2C adrenoceptors was present in subendocardial myocardium (which contains coronary arterioles) but mRNA of {alpha}2A adrenoceptors was not. While mRNA of {alpha}2B adrenoceptors were detected in both coronary arteries and subendocardial myocardium, quantitative analysis revealed that its expression was higher in coronary arteries. Immunohistochemical studies showed that {alpha}2A and {alpha}2B adrenoceptor proteins were located mainly on the endothelial cells of coronary arteries but none was visualized in coronary arterioles. Distributions and cellular localizations of {alpha}2C adrenoceptor proteins had not yet been studied since specific antibody was not available. This preliminary data suggests that coronary blood vessels of different sizes may respond differently to pharmacological agents. | - |
dc.language | eng | en_HK |
dc.publisher | Federation of American Societies for Experimental Biology. Meeting abstracts can be accessed via http://www.fasebj.org/search.dtl | en_HK |
dc.relation.ispartof | The FASEB Journal | en_HK |
dc.title | Distributions of alpha 2 adrenoceptor subtypes in porcine coronary vasculature | en_HK |
dc.type | Conference_Paper | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0176-8638&volume=&spage=&epage=&date=2008&atitle=Distributions+of+alpha+2+adrenoceptor+subtypes+in+porcine+coronary+vasculature.++San+Diego,+5-9+Apr+ | en_HK |
dc.identifier.email | Ho, YW: eywho@graduate.hku.hk | en_HK |
dc.identifier.email | Leung, GPH: leung_pak_heng@hotmail.com | en_HK |
dc.identifier.email | Man, RYK: rykman@hkucc.hku.hk | en_HK |
dc.identifier.email | Vanhoutte, PMGR: vanhoutt@hku.hk | en_HK |
dc.identifier.email | Ng, JKF: jkfng@hkucc.hku.hk | en_HK |
dc.identifier.authority | Leung, GPH=rp00234 | en_HK |
dc.identifier.authority | Man, RYK=rp00236 | en_HK |
dc.identifier.authority | Ng, JKF=rp00544 | en_HK |
dc.identifier.hkuros | 143139 | en_HK |
dc.identifier.hkuros | 168525 | - |
dc.identifier.volume | 22 | - |
dc.identifier.issue | meeting abstract suppl., abstract no. 914.10 | - |
dc.identifier.issnl | 0892-6638 | - |