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Conference Paper: Antiapoptotic effects of mitochondrial manganese superoxide dismutase in ovarian cancer cells

TitleAntiapoptotic effects of mitochondrial manganese superoxide dismutase in ovarian cancer cells
Authors
Issue Date2006
PublisherHong Kong Academy of Medicine
Citation
The 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 170 Abstract no. P212 How to Cite?
AbstractObjective: To investigate the protein expression levels of manganese superoxide dismutase (MnSOD) in ovarian cancer cell lines and ovarian surface epithelial (OSE) cells and the possible correlation between MnSOD expression and resistance to stress-induced apoptosis. Methods: Protein expression levels were measured by immunoblot analysis. Expression of MnSOD was manipulated by overexpression or small interfering RNA. Cell proliferation was examined by cell growth, colony formation, and MTT assays, whereas cell death was measured by DAPI and TUNEL assays. Results: MnSOD expression was significantly higher in most ovarian cancer cell lines than OSE. Overexpression of MnSOD in ovarian cancer cells conferred a strong decrease in cell proliferation and an increase of apoptosis, whereas targeted knockdown of endogenous MnSOD reduced apoptosis. Furthermore, stimulation of mitochondrial superoxide induced an increase of MnSOD expression, suggesting that MnSOD may alleviate the reactive oxygen species stress in these cells. We also showed that overexpression of MnSOD protected ovarian cancer cells against apoptosis induced by treatment of rotenone, hydrogen peroxide, or hypoxia-mimicking agents cobalt chloride and deferoxamine. Conclusion: Upregulation of MnSOD is one of the mechanisms which may increase resistance to oxidative stress and apoptosis in ovarian cancer cells.
Persistent Identifierhttp://hdl.handle.net/10722/110534
ISSN
2023 Impact Factor: 3.1
2023 SCImago Journal Rankings: 0.261

 

DC FieldValueLanguage
dc.contributor.authorWong, KYen_HK
dc.contributor.authorYeung, HYen_HK
dc.contributor.authorWong, ASTen_HK
dc.date.accessioned2010-09-26T02:10:03Z-
dc.date.available2010-09-26T02:10:03Z-
dc.date.issued2006en_HK
dc.identifier.citationThe 4th International Huaxia Congress of Endocrinology, Hong Kong, 15–18 December 2006. In Hong Kong Medical Journal, 2006, v. 12 n. 6 S4, p. 170 Abstract no. P212-
dc.identifier.issn1024-2708-
dc.identifier.urihttp://hdl.handle.net/10722/110534-
dc.description.abstractObjective: To investigate the protein expression levels of manganese superoxide dismutase (MnSOD) in ovarian cancer cell lines and ovarian surface epithelial (OSE) cells and the possible correlation between MnSOD expression and resistance to stress-induced apoptosis. Methods: Protein expression levels were measured by immunoblot analysis. Expression of MnSOD was manipulated by overexpression or small interfering RNA. Cell proliferation was examined by cell growth, colony formation, and MTT assays, whereas cell death was measured by DAPI and TUNEL assays. Results: MnSOD expression was significantly higher in most ovarian cancer cell lines than OSE. Overexpression of MnSOD in ovarian cancer cells conferred a strong decrease in cell proliferation and an increase of apoptosis, whereas targeted knockdown of endogenous MnSOD reduced apoptosis. Furthermore, stimulation of mitochondrial superoxide induced an increase of MnSOD expression, suggesting that MnSOD may alleviate the reactive oxygen species stress in these cells. We also showed that overexpression of MnSOD protected ovarian cancer cells against apoptosis induced by treatment of rotenone, hydrogen peroxide, or hypoxia-mimicking agents cobalt chloride and deferoxamine. Conclusion: Upregulation of MnSOD is one of the mechanisms which may increase resistance to oxidative stress and apoptosis in ovarian cancer cells.-
dc.languageengen_HK
dc.publisherHong Kong Academy of Medicine-
dc.relation.ispartofHong Kong Medical Journalen_HK
dc.titleAntiapoptotic effects of mitochondrial manganese superoxide dismutase in ovarian cancer cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailYeung, HY: bhyyeung@gmail.comen_HK
dc.identifier.emailWong, AST: awong1@hkucc.hku.hken_HK
dc.identifier.authorityWong, AST=rp00805en_HK
dc.identifier.hkuros130205en_HK
dc.identifier.issnl1024-2708-

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