Mechanistic investigation of antimicrobial activity of bismuth tropolone compounds by (metallo)proteomic approaches

Professor Sun, Hongzhe   (Principal Investigator (PI))

Dr Koohimoghadam Mohamad   (Co-Investigator)

Dr Kao Richard Yi Tsun   (Co-Investigator)

36

2021-11-01

666015

Mechanistic investigation of antimicrobial activity of bismuth tropolone compounds by (metallo)proteomic approaches

bioinorganic chemistry, bismuth, medicinal inorganic chemistry, metalloproteomics, proteins

Chemical Sciences

Physical Sciences (P)

17308921

General Research Fund (GRF)

2021

On-going

1 To determine MIC of Bi(III) tropolone against Staphylococcus aureus, and monitor time-dependent Bi(III) cellular uptake in bacterial cells 2 To identify Bi(III)-binding proteins in bacterial cells upon treatment of bacterium with sub-lethal concentration of Bi(III) tropolone by our established continuous-flow gel electrophoresis coupled with inductively-coupled plasma mass spectrometry 3 To profile Bi(III)-regulated proteins by MS-based quantitative proteomic approach 4 To uncover the biological pathways perturbed by Bi(III) tropolone and to explore protein-protein interaction network of Bi(III)-binding and regulated proteins to unveil hub proteins by bioinformatics 5 To subsequently validate key protein targets and the key biological pathways perturbed by Bi(III) tropolone through either bioassays or purified proteins using various biophysical and molecular biology methods