Evaluation of the role of a single amino acid substitution associated with inter-species transmission of MERS-coronavirus from camels to humans
Grant Data
Project Title
Evaluation of the role of a single amino acid substitution associated with inter-species transmission of MERS-coronavirus from camels to humans
Principal Investigator
Professor Peiris, Joseph Sriyal Malik
(Principal Investigator (PI))
Co-Investigator(s)
Dr Mok Ka Pun
(Co-Investigator)
Professor Zhao Jincun
(Co-Investigator)
Dr Chu Ka Wing
(Co-Investigator)
Professor Poon Lit Man Leo
(Co-Investigator)
Duration
24
Start Date
2020-04-01
Completion Date
2023-08-24
Amount
1449502
Conference Title
Evaluation of the role of a single amino acid substitution associated with inter-species transmission of MERS-coronavirus from camels to humans
Keywords
adaptation, coronavirus, evolution, MERS, mutation, zoonosis
Discipline
Others - Medicine, Dentistry and Health
HKU Project Code
19181032
Grant Type
Health and Medical Research Fund - Full Grant
Funding Year
2019
Status
Completed
Objectives
1) To generate isogenic infectious clones of a MERS-CoV (Korean lineage) virus which have ether AA nsp6 232F or 232L; and to phenotype these isogenic viruses in regard to replication competence in 2) human respiratory epithelial cell lines, 3) ex vivo cultures of the human lung and bronchus and in 4) lungs of human DPP4 transgenic mice. The biological role of nsp6 L232F on double membrane vesicle formation and innate immune evasion will be investigated.