Development of ultralarge-scale, multiparametric spinning imaging mechanocytometry (SIMeC)
Grant Data
Project Title
Development of ultralarge-scale, multiparametric spinning imaging mechanocytometry (SIMeC)
Principal Investigator
Professor Tsia, Kevin Kin Man
(Principal Investigator (PI))
Duration
36
Start Date
2019-01-01
Completion Date
2021-12-31
Amount
495086
Conference Title
Development of ultralarge-scale, multiparametric spinning imaging mechanocytometry (SIMeC)
Presentation Title
Keywords
Biophotonics, High-speed optical imaging
Discipline
Photonics,Others - Electrical and Electronic Engineering
Panel
Engineering (E)
HKU Project Code
17208918
Grant Type
General Research Fund (GRF)
Funding Year
2018
Status
Completed
Objectives
1) - Develop the microfluidic spinning disk assay platform for SIMeC – We will design and fabricate a microfluidic spinning-disk adherent-cell assay platform that harnesses the centrifugal microfluidic concept to enable both shear-stress flow cell assay for measuring cell stiffness; and traction force microscopy for quantifying cell traction force; 2) Establish the SIMeC prototype: from instrumentation to phenotypic analysis – We will develop a multi-modal (quantitative phase and fluorescence) ultrafast FACED microscopy system and combine it with the spinning-disk assay platform for ultralarge-scale SIMeC. We will perform proof-of-principle experiments to evaluate the robustness of high-dimensional image-derived cellular phenotyping by SIMeC; 3) Explore the potential applications of SIMeC – We will employ the SIMeC prototype for exploring its utilities in clinical diagnostic (label-free screening of T-cell activation for cancer immunotherapy) and basic biology research (ultralarge-scale mechanophenotyping of stem cell differentiation).