Systemic approach for uncovering the roles of metals in biology and medicine: mechanism of actions of antimicrobial bismuth drugs

Professor Sun, Hongzhe   (Principal Investigator (PI))

Dr Chu Ivan Keung   (Co-Investigator)

36

2017-09-01

522898

Systemic approach for uncovering the roles of metals in biology and medicine: mechanism of actions of antimicrobial bismuth drugs

bioinformatics, bismuth, metabolomics, metalloproteomics, protein targets

Chemical Sciences,Others - Physical Sciences

Physical Sciences (P)

17307017

General Research Fund (GRF)

2017

Completed

1 To identify bismuth binding proteins in both H. pylori and other pathogens by a home-made integrative approach consisting of a fluorescence probe and continuous flow gel electrophoresis ICP-MS (GE-ICP-MS); 2 To carry out quantitative proteomics to quantify those proteins up- and down-regulated by bismuth antimicrobial drugs in both H. pylori and related pathogns; 3 To carry out quantitative proteomics (iTRAQ) to quantify those proteins up- and down-regulated by bismuth antimicrobial drugs in both H. pylori and other pathogens (C. jejuni); 4 To identify important low molecular mass metabolites upon the drug treatment by NMR-based metabolomics and validate the key proteins/enzymes or biological pathways perturbed; 5 To verify the identified key targets identified by metalloproteomics and metabolomics by in vivo bioassay and/or in vitro characterization.