Biophysical/biochemical and functional validation of putative bismuth targets in Helicobacter pylori to elucidate the molecular mechanism of action of bismuth drugs
Grant Data
Project Title
Biophysical/biochemical and functional validation of putative bismuth targets in Helicobacter pylori to elucidate the molecular mechanism of action of bismuth drugs
Principal Investigator
Professor Sun, Hongzhe
(Principal Investigator (PI))
Duration
36
Start Date
2016-10-01
Amount
540824
Conference Title
Biophysical/biochemical and functional validation of putative bismuth targets in Helicobacter pylori to elucidate the molecular mechanism of action of bismuth drugs
Presentation Title
Keywords
bismuth, Helicobacter pylori, proteins, structure and function
Discipline
Chemical Sciences,Others - Physical Sciences
Panel
Physical Sciences (P)
HKU Project Code
17333616
Grant Type
General Research Fund (GRF)
Funding Year
2016
Status
Completed
Objectives
1 To perform molecular cloning of the putative protein targets of bismuth antimicrobial drugs, and to overexpress and purify the proteins including Ahpc, UreG, Fur and Dnak 2 To examine the in vitro binding properties including binding stoichiometry, affinity of bismuth drugs with the proteins by biochemical and biophysical techniques 3 To investigate the effect of bismuth drugs on the function of selected protein targets through bioassays 4 To further validate the potential disruption of bismuth drugs on the protein functions in vivo by gene knockout