Radical-Mediated Protein Tyrosine Nitration In Vavuo: Experimental and Theoretical Examination

Dr Chu, Ivan Keung   (Principal Investigator (PI))

Dr Siu Chi Kit   (Co-Investigator)

Dr Laskin Julia   (Co-Investigator)

36

2016-08-01

540824

Radical-Mediated Protein Tyrosine Nitration In Vavuo: Experimental and Theoretical Examination

gas-phase chemistry, Protein Tyrosine Nitration, Radical peptide

Mass Spectrometry,Chemical Sciences

Physical Sciences (P)

17318616

General Research Fund (GRF)

2016

Completed

1 To design, generate, and characterize peptide systems leading to the gas phase formation of stable 3-nitrotyrosyl-containing peptides through ion–molecule reactions of tyrosine-containing peptide radical cations with •NO2; in Year 1. 2 To elucidate the mechanistic, energetic, and dynamic details of the multiple steps involving intermediate radical ion structures prior to their radical recombination reactions, using ion–molecule reactions, low-energy collision-induced dissociation experiments, density functional theory calculations, and Rice–Ramsperger–Kassel–Marcus modeling in combination with surface-induced dissociation experiments; throughout Years 1, 2, and 3. 3 To initiate an exploration of potential relevance to endogenous protein tyrosine nitration; in Years 2 and 3.