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Conference Paper: Inhibition of Melanoma Development by Single Dose Administration of hTERTC27 Viral Cocktail in C57BL/6 Mice

TitleInhibition of Melanoma Development by Single Dose Administration of hTERTC27 Viral Cocktail in C57BL/6 Mice
Authors
Issue Date2007
PublisherElsevier Inc.
Citation
American Society of Gene Therapy 10th Annual Meeting, Seattle, WA, 30 May-3 June 2007. In Molecular Therapy, 2007, v. 15 n. S1, p. S89 How to Cite?
AbstracthTERTC27, a 27 kDa C-terminal polypeptide of human telomerase reverse transcriptase, has been previously demonstrated to reduce the tumorigenicity of HeLa cells. We report here that a rAAV-/rAdvviral cocktail expressing hTERTC27 peptide can induce growth inhibition of transplanted melanoma tumors in immunocompetent C57BL/6 mice. Melanoma B16 tumor growth was significantly inhibited by subcutaneous administration with a single dose of 1.5 ×1011 vg rAAV-hTERTC27 and 2.5×109 pfu rAdv-hTERTC27 viral cocktail (rAAV-/rAdv-hTERTC27). The population of NK cells and the levels of cytokines, including IL-2, IFN-g and GM-CSF, in serum were markedly increased after administration of the hTERTC27 rAAW-/rAdv-viral cocktail. The specific cytotoxicity of NK cells in mice treated with rAAV-/rAdv-hTERTC27 viral cocktail was also significantly higher than that observed in control mice treated with rAAV-/rAdv-EGFP viral cocktail and PBS. These results, demonstrated for the first time that the subcutaneous administration of rAAV-/rAdv-hTERTC27 viral cocktail could induce strong innate immunity against melanoma. Furthermore, single dose injection of rAAV-rAdv-hTERTC27 exhibited no detectable toxicity. This indicates that rAAV-rAdv hTERTC27 might be a suitable palliative and system therapy in patients with advanced melanoma.
Persistent Identifierhttp://hdl.handle.net/10722/97199
ISSN
2015 Impact Factor: 6.938
2015 SCImago Journal Rankings: 3.374

 

DC FieldValueLanguage
dc.contributor.authorWang, Xen_HK
dc.contributor.authorHuo, Len_HK
dc.contributor.authorYao, Hen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLin, MCen_HK
dc.date.accessioned2010-09-25T17:00:05Z-
dc.date.available2010-09-25T17:00:05Z-
dc.date.issued2007en_HK
dc.identifier.citationAmerican Society of Gene Therapy 10th Annual Meeting, Seattle, WA, 30 May-3 June 2007. In Molecular Therapy, 2007, v. 15 n. S1, p. S89en_HK
dc.identifier.issn1525-0016-
dc.identifier.urihttp://hdl.handle.net/10722/97199-
dc.description.abstracthTERTC27, a 27 kDa C-terminal polypeptide of human telomerase reverse transcriptase, has been previously demonstrated to reduce the tumorigenicity of HeLa cells. We report here that a rAAV-/rAdvviral cocktail expressing hTERTC27 peptide can induce growth inhibition of transplanted melanoma tumors in immunocompetent C57BL/6 mice. Melanoma B16 tumor growth was significantly inhibited by subcutaneous administration with a single dose of 1.5 ×1011 vg rAAV-hTERTC27 and 2.5×109 pfu rAdv-hTERTC27 viral cocktail (rAAV-/rAdv-hTERTC27). The population of NK cells and the levels of cytokines, including IL-2, IFN-g and GM-CSF, in serum were markedly increased after administration of the hTERTC27 rAAW-/rAdv-viral cocktail. The specific cytotoxicity of NK cells in mice treated with rAAV-/rAdv-hTERTC27 viral cocktail was also significantly higher than that observed in control mice treated with rAAV-/rAdv-EGFP viral cocktail and PBS. These results, demonstrated for the first time that the subcutaneous administration of rAAV-/rAdv-hTERTC27 viral cocktail could induce strong innate immunity against melanoma. Furthermore, single dose injection of rAAV-rAdv-hTERTC27 exhibited no detectable toxicity. This indicates that rAAV-rAdv hTERTC27 might be a suitable palliative and system therapy in patients with advanced melanoma.-
dc.languageengen_HK
dc.publisherElsevier Inc.-
dc.relation.ispartofMolecular Therapyen_HK
dc.titleInhibition of Melanoma Development by Single Dose Administration of hTERTC27 Viral Cocktail in C57BL/6 Miceen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWang, X: wangxc2005323@126.comen_HK
dc.identifier.emailHuo, L: huolf@yahoo.com.hken_HK
dc.identifier.emailLin, MC: mcllin@HKUCC.hku.hken_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1525-0016(16)44440-0-
dc.identifier.hkuros126375en_HK
dc.identifier.spageS89en_HK

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