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Conference Paper: Immunohistochemical localization of chondroitin sulfate proteoglycans in the developing rat hindbrain

TitleImmunohistochemical localization of chondroitin sulfate proteoglycans in the developing rat hindbrain
Authors
Issue Date2006
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSG
Citation
The Hong Kong Society of Neurosciences 24th Annual Scientific Meeting, Hong Kong, 13-14 January 2005. In Neurosignals, 2006, v. 15 n. 3, p. 130 How to Cite?
AbstractOur biochemical analysis has shown the occurrence of chondroitin sulfate proteoglycans (CSPGs) in the hindbrain of rat embryos. To localize their expression in the embryos, immunohistochemistry was applied on cryosections of E11.5, E12.5 and E13.5 embryos. Epitopes of CS were detectable in the extra/peri-cellular environment of the neuroepithelium at E11.5 and E12.5. Signals were very weak by E13.5. The results were confirmed by the disappearance of signals following chondroitinase treatment of the sections. Versican-positive signals were pericellular in all three stages. The immunoreactivity was strong towards the ventricular region at E11.5. The signals declined by E12.5 and strong immunoreactivity was found towards the pial surfaces. Neurocan-positive signals appeared to be intracellular and concentrated in a cell layer lining the pial side of the neural tube as early as E12.5. Phosphacan-positive signals were not detectable in all three stages. As hyaluronan binds CSPGs, experiments were also performed to localize hyaluronan in the embryos. The hyaluronan staining pattern resembled that of versican in all three stages, suggesting interaction between versican and hyaluronan in the development of the embryonic hindbrain.
Persistent Identifierhttp://hdl.handle.net/10722/96720
ISSN
2015 Impact Factor: 1.593
2015 SCImago Journal Rankings: 0.763

 

DC FieldValueLanguage
dc.contributor.authorWang, Fen_HK
dc.contributor.authorKwok, JCFen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-25T16:42:35Z-
dc.date.available2010-09-25T16:42:35Z-
dc.date.issued2006en_HK
dc.identifier.citationThe Hong Kong Society of Neurosciences 24th Annual Scientific Meeting, Hong Kong, 13-14 January 2005. In Neurosignals, 2006, v. 15 n. 3, p. 130en_HK
dc.identifier.issn1424-862Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/96720-
dc.description.abstractOur biochemical analysis has shown the occurrence of chondroitin sulfate proteoglycans (CSPGs) in the hindbrain of rat embryos. To localize their expression in the embryos, immunohistochemistry was applied on cryosections of E11.5, E12.5 and E13.5 embryos. Epitopes of CS were detectable in the extra/peri-cellular environment of the neuroepithelium at E11.5 and E12.5. Signals were very weak by E13.5. The results were confirmed by the disappearance of signals following chondroitinase treatment of the sections. Versican-positive signals were pericellular in all three stages. The immunoreactivity was strong towards the ventricular region at E11.5. The signals declined by E12.5 and strong immunoreactivity was found towards the pial surfaces. Neurocan-positive signals appeared to be intracellular and concentrated in a cell layer lining the pial side of the neural tube as early as E12.5. Phosphacan-positive signals were not detectable in all three stages. As hyaluronan binds CSPGs, experiments were also performed to localize hyaluronan in the embryos. The hyaluronan staining pattern resembled that of versican in all three stages, suggesting interaction between versican and hyaluronan in the development of the embryonic hindbrain.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NSGen_HK
dc.relation.ispartofNeurosignalsen_HK
dc.rightsNeurosignals. Copyright © S Karger AG.en_HK
dc.titleImmunohistochemical localization of chondroitin sulfate proteoglycans in the developing rat hindbrainen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1424-862X&volume=15&spage=130&epage=130&date=2006&atitle=Immunohistochemical+localization+of+chondroitin+sulfate+proteoglycans+in+the+developing+rat+hindbrainen_HK
dc.identifier.emailKwok, JCF: h9600218@hkusua.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000095356-
dc.identifier.hkuros129730en_HK
dc.identifier.volume15en_HK
dc.identifier.spage130en_HK
dc.identifier.epage130en_HK

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