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Conference Paper: A vagal neural crest specific enhancer for establishing transgenic mouse models

TitleA vagal neural crest specific enhancer for establishing transgenic mouse models
Authors
Issue Date2001
PublisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/DGD
Citation
The 14th International Congress of Developmental Biology, Kyoto, Japan, 8-12 July 2001, In Development, Growth and Differentiation, 2001, v. 43 suppl. 1, p. S77, abstract no. S8-P32 How to Cite?
AbstractThe enteric nervous system (ENS) is originated from the vagal neural crest (VNC) but the sacral neural crest (SNC) also has some contribution. Cell ablation and transplantation studies in avian species showed that the VNC colonizes the entire gut in a rostrocaudal direction while the SNC colonizes only the hindgut. However, the coordination of neural crest migration and the molecular control of their differentiation into specific cell types are not clearly known. We have identified a VNC specific enhancer element from the mouse Hoxb3 gene, b3-IIIa, which could direct lacZ reporter gene expression in the ENS of transgenic mice. When crossed with the mega- colon mutant Dom, the hybrid mutant displayed an absence of lacZ marked cells in the hindgut, further confirming the specificity of this enhancer in the ENS. To allow further studies of genes required for normal ENS development by conditional gene targeting, we generated IIIa-cre transgenic mouse lines using the VNC enhancer. Tissue specificity and efficiency of the cre recombinase was assess by crossing the IIIa-cre transgenic mice with the Z/AP reporter mouse line.
Persistent Identifierhttp://hdl.handle.net/10722/96710
ISSN
2015 Impact Factor: 2.412
2015 SCImago Journal Rankings: 1.561

 

DC FieldValueLanguage
dc.contributor.authorTsang, WHen_HK
dc.contributor.authorChen, YSen_HK
dc.contributor.authorSham, MHen_HK
dc.date.accessioned2010-09-25T16:42:17Z-
dc.date.available2010-09-25T16:42:17Z-
dc.date.issued2001en_HK
dc.identifier.citationThe 14th International Congress of Developmental Biology, Kyoto, Japan, 8-12 July 2001, In Development, Growth and Differentiation, 2001, v. 43 suppl. 1, p. S77, abstract no. S8-P32en_HK
dc.identifier.issn0012-1592en_HK
dc.identifier.urihttp://hdl.handle.net/10722/96710-
dc.description.abstractThe enteric nervous system (ENS) is originated from the vagal neural crest (VNC) but the sacral neural crest (SNC) also has some contribution. Cell ablation and transplantation studies in avian species showed that the VNC colonizes the entire gut in a rostrocaudal direction while the SNC colonizes only the hindgut. However, the coordination of neural crest migration and the molecular control of their differentiation into specific cell types are not clearly known. We have identified a VNC specific enhancer element from the mouse Hoxb3 gene, b3-IIIa, which could direct lacZ reporter gene expression in the ENS of transgenic mice. When crossed with the mega- colon mutant Dom, the hybrid mutant displayed an absence of lacZ marked cells in the hindgut, further confirming the specificity of this enhancer in the ENS. To allow further studies of genes required for normal ENS development by conditional gene targeting, we generated IIIa-cre transgenic mouse lines using the VNC enhancer. Tissue specificity and efficiency of the cre recombinase was assess by crossing the IIIa-cre transgenic mice with the Z/AP reporter mouse line.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Asia. The Journal's web site is located at http://www.blackwellpublishing.com/journals/DGDen_HK
dc.relation.ispartofDevelopment, Growth and Differentiationen_HK
dc.titleA vagal neural crest specific enhancer for establishing transgenic mouse modelsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0012-1592&volume=43&issue=Suppl.&spage=S77&epage=&date=2001&atitle=A+vagal+neural+crest+specific+enhancer+for+establishing+transgenic+mouse+modelsen_HK
dc.identifier.emailSham, MH: mhsham@hkucc.hku.hken_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1046/j.1440-169X.2001.00003-Supplement.x-
dc.identifier.hkuros63614en_HK
dc.identifier.volume43en_HK
dc.identifier.issuesuppl.en_HK
dc.identifier.spageS77, abstract no. S8-P32en_HK
dc.identifier.epageS77, abstract no. S8-P32-

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