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Conference Paper: Molecular cloning and characterization of chondroitin 6-sulfotransferase (c6st) in crush-injured rat sciatic nerves

TitleMolecular cloning and characterization of chondroitin 6-sulfotransferase (c6st) in crush-injured rat sciatic nerves
Authors
KeywordsC6ST
enzyme
injury
regeneration
Issue Date2002
PublisherSociety for Neuroscience
Citation
Neuroscience 2002, Orlando, FL, 3-7 November 2002, Presentation no. 529.16 How to Cite?
AbstractOur earlier results indicated upregulated expression of chondroitin 6-sulfotransferase (C6ST) in crushed nerves and thus provided suggestion for the cause of increase of chondroitin 6 sulfate isoforms in the crushed nerve environment. To verify this, we cloned the full length C6ST from RNAs recovered from injured rat sciatic nerves. Sequencing results predicted a protein of 474 amino acids with homologies of 84% and 93% to those of human and mouse C6ST respectively. Transient transfection of expression constructs carrying either the full-length or a truncated isoform of C6ST into COS-7 cells resulted in increased C6ST activity in the cell homogenates. The recombinant C6ST was verified to transfer sulfate to carbon-6 of N-acetylgalactosamine residues of chondroitin. In situ hybridization with DIG-labeled C6ST cRNA probe revealed positive signals in Schwann cells and increased signals in crushed sciatic nerves, suggesting a functional role in nerve regeneration. (SPON: The Hong Kong Society of Neurosciences). Supported by HKU 7294/01M
Persistent Identifierhttp://hdl.handle.net/10722/96706

 

DC FieldValueLanguage
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorChau, CHen_HK
dc.contributor.authorChung, SKen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-25T16:42:10Z-
dc.date.available2010-09-25T16:42:10Z-
dc.date.issued2002en_HK
dc.identifier.citationNeuroscience 2002, Orlando, FL, 3-7 November 2002, Presentation no. 529.16en_HK
dc.identifier.urihttp://hdl.handle.net/10722/96706-
dc.description.abstractOur earlier results indicated upregulated expression of chondroitin 6-sulfotransferase (C6ST) in crushed nerves and thus provided suggestion for the cause of increase of chondroitin 6 sulfate isoforms in the crushed nerve environment. To verify this, we cloned the full length C6ST from RNAs recovered from injured rat sciatic nerves. Sequencing results predicted a protein of 474 amino acids with homologies of 84% and 93% to those of human and mouse C6ST respectively. Transient transfection of expression constructs carrying either the full-length or a truncated isoform of C6ST into COS-7 cells resulted in increased C6ST activity in the cell homogenates. The recombinant C6ST was verified to transfer sulfate to carbon-6 of N-acetylgalactosamine residues of chondroitin. In situ hybridization with DIG-labeled C6ST cRNA probe revealed positive signals in Schwann cells and increased signals in crushed sciatic nerves, suggesting a functional role in nerve regeneration. (SPON: The Hong Kong Society of Neurosciences). Supported by HKU 7294/01M-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.subjectC6ST-
dc.subjectenzyme-
dc.subjectinjury-
dc.subjectregeneration-
dc.titleMolecular cloning and characterization of chondroitin 6-sulfotransferase (c6st) in crush-injured rat sciatic nervesen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLiu, J: liuj@hkusua.hku.hken_HK
dc.identifier.emailChau, CH: mchchau@hkucc.hku.hken_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityChau, CH=rp00398en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.hkuros84415en_HK

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