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Conference Paper: The anti-proliferative effects of sPDZD2 on prostate and non-prostate cancer cells

TitleThe anti-proliferative effects of sPDZD2 on prostate and non-prostate cancer cells
Authors
Issue Date2007
PublisherFederation of American Societies for Experimental Biology.
Citation
The FASEB Journal, 2007, v. 21 n. 5, p. A387 How to Cite?
AbstractThe PDZ domain containing protein 2 (PDZD2) is believed to be involved in the prostate cancer development and it can be cleaved by caspase-3 to produce a 37kDa secreted protein sPDZD2 in prostate cancer cell lines. Our laboratory has previously shown that sPDZD2 can inhibit the proliferation of DU145 prostate cancer cells and promote apoptosis in LNCaP prostate cancer cells. By using a nude mice xenograft model, we investigated the effects of sPDZD2 under in vivo conditions. DU145 cells were subcutaneously injected into nude mice, and the mice were either intraperitoneally injected daily with low dose (0.084 mg) or high dose (8.4 mg) of recombinant sPDZD2. The volumes of the tumors were then measured weekly. There was a significant decrease in tumor volume in nude mice treated with high dose (8.4 mg) sPDZD2 as compared with the control group. Moreover, the biological effects of sPDZD2 on human non-prostate cancer cells were examined. Using cell count and MTS assays, it was found that 10 nM and 100 nM of sPDZD2 induced anti-proliferative effects on hepatocellular carcinoma Hep-G2 cells and hormone-sensitive breast adenocarcinoma MCF-7 cells, but not colon adenocarcinoma LoVo cells, renal carcinoma 786-O cells, hepatocellualr carcinoma Hep-3B cells and hormone-insensitive breast adenocarcinoma MDA-MB-468 cells. The work was supported by Research Grants HKU7474/04M (to K-MY) and the HKU7580/05M (to SYWS).
Persistent Identifierhttp://hdl.handle.net/10722/96660
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorCheng, SAen_HK
dc.contributor.authorTam, CWen_HK
dc.contributor.authorYao, KMen_HK
dc.contributor.authorShiu, SYWen_HK
dc.date.accessioned2010-09-25T16:40:45Z-
dc.date.available2010-09-25T16:40:45Z-
dc.date.issued2007en_HK
dc.identifier.citationThe FASEB Journal, 2007, v. 21 n. 5, p. A387en_HK
dc.identifier.issn0892-6638en_HK
dc.identifier.urihttp://hdl.handle.net/10722/96660-
dc.description.abstractThe PDZ domain containing protein 2 (PDZD2) is believed to be involved in the prostate cancer development and it can be cleaved by caspase-3 to produce a 37kDa secreted protein sPDZD2 in prostate cancer cell lines. Our laboratory has previously shown that sPDZD2 can inhibit the proliferation of DU145 prostate cancer cells and promote apoptosis in LNCaP prostate cancer cells. By using a nude mice xenograft model, we investigated the effects of sPDZD2 under in vivo conditions. DU145 cells were subcutaneously injected into nude mice, and the mice were either intraperitoneally injected daily with low dose (0.084 mg) or high dose (8.4 mg) of recombinant sPDZD2. The volumes of the tumors were then measured weekly. There was a significant decrease in tumor volume in nude mice treated with high dose (8.4 mg) sPDZD2 as compared with the control group. Moreover, the biological effects of sPDZD2 on human non-prostate cancer cells were examined. Using cell count and MTS assays, it was found that 10 nM and 100 nM of sPDZD2 induced anti-proliferative effects on hepatocellular carcinoma Hep-G2 cells and hormone-sensitive breast adenocarcinoma MCF-7 cells, but not colon adenocarcinoma LoVo cells, renal carcinoma 786-O cells, hepatocellualr carcinoma Hep-3B cells and hormone-insensitive breast adenocarcinoma MDA-MB-468 cells. The work was supported by Research Grants HKU7474/04M (to K-MY) and the HKU7580/05M (to SYWS).-
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology.en_HK
dc.relation.ispartofThe FASEB Journalen_HK
dc.titleThe anti-proliferative effects of sPDZD2 on prostate and non-prostate cancer cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0892-6638&volume=21 &issue=5&spage=A387 &epage=&date=2007&atitle=The+anti-proliferative+effects+of+sPDZD2+on+prostate+and+non-prostate+cancer+cellsen_HK
dc.identifier.emailYao, KM: kmyao@hkusua.hku.hken_HK
dc.identifier.emailShiu, SYW: sywshiu@hkucc.hku.hken_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.identifier.authorityShiu, SYW=rp00384en_HK
dc.identifier.hkuros127158en_HK
dc.identifier.volume21en_HK
dc.identifier.issue5en_HK
dc.identifier.spageA387en_HK
dc.identifier.epageA387-

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