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Conference Paper: Expression of chondroitin sulfotransferases in the injured spinal cord

TitleExpression of chondroitin sulfotransferases in the injured spinal cord
Authors
Keywordschondroitin sulfotransferase
proteoglycan
injured spinal cord
Issue Date2004
PublisherSociety for Neuroscience
Citation
Neuroscience 2004, San Diego, CA, 23-27 October 2004, Presentation no. 43.6 How to Cite?
AbstractWe demonstrated that chondroitinase ABC infused into the graft-host interface resolved the glial scar and improved prospects of axonal regeneration into the distal host spinal cord. To identify the cellular source(s) of chondroitin sulfates that contribute to the glial scar, we mapped the expression of chondroitin sulfotransferases in relation to reactive cells about a lateral hemisection of the thoracic cord (T8) of adult Sprague Dawley rats. Riboprobes for chondroitin 6-sulfotransferase (C6ST), chondroitin 4-sulfotransferases (C4ST-1 and C4ST-2) and uronyl 2-sulfotransferase (U2ST) were generated according to reported sequences of the respective cDNAs. In the normal cord, mRNAs of C6ST and C4ST-1 were detected in GFAP-positive astrocytes, RIP-positive oligodendrocytes and OX42-positive microglia. In the lesion epicenter, upregulated expresson of C6ST, C4ST-2 and U2ST transcripts were detected in hypertrophic astrocytes; expression of C6ST and C4ST-1 transcripts were also found in activated (ED1-positive) macrophages. The increased signal intensities were detectable as early as 3 days post-lesion and remained so in the 7-d and 14-d post-lesion cord. The differential expression of chondroitin sulfotransferases in various reactive cells are implicated in the production of chondroitin isoforms of proteoglycan deposits in the gliotic matrix about the lesion. (Supported by RGC Grant #7355/00M).
Persistent Identifierhttp://hdl.handle.net/10722/96570

 

DC FieldValueLanguage
dc.contributor.authorChau, CHen_HK
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorShum, DKYen_HK
dc.date.accessioned2010-09-25T16:37:56Z-
dc.date.available2010-09-25T16:37:56Z-
dc.date.issued2004en_HK
dc.identifier.citationNeuroscience 2004, San Diego, CA, 23-27 October 2004, Presentation no. 43.6en_HK
dc.identifier.urihttp://hdl.handle.net/10722/96570-
dc.description.abstractWe demonstrated that chondroitinase ABC infused into the graft-host interface resolved the glial scar and improved prospects of axonal regeneration into the distal host spinal cord. To identify the cellular source(s) of chondroitin sulfates that contribute to the glial scar, we mapped the expression of chondroitin sulfotransferases in relation to reactive cells about a lateral hemisection of the thoracic cord (T8) of adult Sprague Dawley rats. Riboprobes for chondroitin 6-sulfotransferase (C6ST), chondroitin 4-sulfotransferases (C4ST-1 and C4ST-2) and uronyl 2-sulfotransferase (U2ST) were generated according to reported sequences of the respective cDNAs. In the normal cord, mRNAs of C6ST and C4ST-1 were detected in GFAP-positive astrocytes, RIP-positive oligodendrocytes and OX42-positive microglia. In the lesion epicenter, upregulated expresson of C6ST, C4ST-2 and U2ST transcripts were detected in hypertrophic astrocytes; expression of C6ST and C4ST-1 transcripts were also found in activated (ED1-positive) macrophages. The increased signal intensities were detectable as early as 3 days post-lesion and remained so in the 7-d and 14-d post-lesion cord. The differential expression of chondroitin sulfotransferases in various reactive cells are implicated in the production of chondroitin isoforms of proteoglycan deposits in the gliotic matrix about the lesion. (Supported by RGC Grant #7355/00M).-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meetingen_HK
dc.subjectchondroitin sulfotransferase-
dc.subjectproteoglycan-
dc.subjectinjured spinal cord-
dc.titleExpression of chondroitin sulfotransferases in the injured spinal corden_HK
dc.typeConference_Paperen_HK
dc.identifier.emailChau, CH: mchchau@hkucc.hku.hken_HK
dc.identifier.emailLiu, J: liuj@hkusua.hku.hken_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_HK
dc.identifier.authorityChau, CH=rp00398en_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.identifier.authorityShum, DKY=rp00321en_HK
dc.identifier.hkuros115363en_HK

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